A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle

Autores
Bellido, Demian; Baztarrica, Josefina; Rocha, Lucía Alejandra; Pecora, Andrea; Acosta, Mario; Escribano, José M.; Parreño, Gladys; Wigdorovitz, Andres
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Bovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two primary methods used to control the spread of BVDV: the elimination of persistently infected (PI) animals and vaccination. Currently, modified live or inactivated vaccines are used in BVDV vaccination programmes, but there are safety risks or insufficient protection, respectively, with these vaccines. Here, we report the development and efficacy of the first targeted subunit vaccine against BVDV. The core of the vaccine is the fusion of the BVDV structural protein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present on antigen-presenting cells. Industrial production of the vaccine is carried out using the baculovirus expression vector system (BEVS) using single-use manufacturing technologies. This new subunit vaccine induces strong BVDV-specific neutralizing antibodies in guinea pigs and cattle. Importantly, in cattle with low levels of natural BVDV-specific neutralizing antibodies, the vaccine induced strong neutralizing antibody levels to above the protective threshold, as determined by a competition ELISA. The APCH-E2 vaccine induced a rapid and sustained neutralizing antibody response compared with a conventional vaccine in cattle.
Instituto de Virologia
Fil: Bellido, Demian. Vetanco SA; Argentina
Fil: Bellido, Demian. Bioinnovo SA; Argentina
Fil: Baztarrica, Josefina. Vetanco SA; Argentina
Fil: Baztarrica, Josefina. Bioinnovo SA; Argentina
Fil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Rocha, Lucía Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Pecora, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acosta, Mario. Vetanco SA; Argentina
Fil: Escribano, José M. Algenex; España
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Parreño, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parreño, Viviana. Bioinnovo S.A.; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Wigdorovitz, Andres. Bioinnovo S.A.; Argentina
Fuente
Transboundary and Emerging Diseases 68 (6) : 3474-3481 (November 2021)
Materia
Pestivirus de la Diarrea Bovina
Vacuna
Respuesta Inmunológica
Ganado Bovino
Cobaya
Bovine Diarrhoea Pestivirus
Vaccines
Immune Response
Cattle
Guinea Pigs
BVDV
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13907
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oai_identifier_str oai:localhost:20.500.12123/13907
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattleBellido, DemianBaztarrica, JosefinaRocha, Lucía AlejandraPecora, AndreaAcosta, MarioEscribano, José M.Parreño, GladysWigdorovitz, AndresPestivirus de la Diarrea BovinaVacunaRespuesta InmunológicaGanado BovinoCobayaBovine Diarrhoea PestivirusVaccinesImmune ResponseCattleGuinea PigsBVDVBovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two primary methods used to control the spread of BVDV: the elimination of persistently infected (PI) animals and vaccination. Currently, modified live or inactivated vaccines are used in BVDV vaccination programmes, but there are safety risks or insufficient protection, respectively, with these vaccines. Here, we report the development and efficacy of the first targeted subunit vaccine against BVDV. The core of the vaccine is the fusion of the BVDV structural protein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present on antigen-presenting cells. Industrial production of the vaccine is carried out using the baculovirus expression vector system (BEVS) using single-use manufacturing technologies. This new subunit vaccine induces strong BVDV-specific neutralizing antibodies in guinea pigs and cattle. Importantly, in cattle with low levels of natural BVDV-specific neutralizing antibodies, the vaccine induced strong neutralizing antibody levels to above the protective threshold, as determined by a competition ELISA. The APCH-E2 vaccine induced a rapid and sustained neutralizing antibody response compared with a conventional vaccine in cattle.Instituto de VirologiaFil: Bellido, Demian. Vetanco SA; ArgentinaFil: Bellido, Demian. Bioinnovo SA; ArgentinaFil: Baztarrica, Josefina. Vetanco SA; ArgentinaFil: Baztarrica, Josefina. Bioinnovo SA; ArgentinaFil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; ArgentinaFil: Rocha, Lucía Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; ArgentinaFil: Pecora, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Acosta, Mario. Vetanco SA; ArgentinaFil: Escribano, José M. Algenex; EspañaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; ArgentinaFil: Parreño, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Parreño, Viviana. Bioinnovo S.A.; ArgentinaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; ArgentinaFil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Wigdorovitz, Andres. Bioinnovo S.A.; ArgentinaWiley2023-01-13T13:50:39Z2023-01-13T13:50:39Z2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13907https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.139521865-16741865-1682https://doi.org/10.1111/tbed.13952Transboundary and Emerging Diseases 68 (6) : 3474-3481 (November 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccess2025-09-04T09:49:42Zoai:localhost:20.500.12123/13907instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:43.139INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
title A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
spellingShingle A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
Bellido, Demian
Pestivirus de la Diarrea Bovina
Vacuna
Respuesta Inmunológica
Ganado Bovino
Cobaya
Bovine Diarrhoea Pestivirus
Vaccines
Immune Response
Cattle
Guinea Pigs
BVDV
title_short A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
title_full A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
title_fullStr A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
title_full_unstemmed A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
title_sort A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
dc.creator.none.fl_str_mv Bellido, Demian
Baztarrica, Josefina
Rocha, Lucía Alejandra
Pecora, Andrea
Acosta, Mario
Escribano, José M.
Parreño, Gladys
Wigdorovitz, Andres
author Bellido, Demian
author_facet Bellido, Demian
Baztarrica, Josefina
Rocha, Lucía Alejandra
Pecora, Andrea
Acosta, Mario
Escribano, José M.
Parreño, Gladys
Wigdorovitz, Andres
author_role author
author2 Baztarrica, Josefina
Rocha, Lucía Alejandra
Pecora, Andrea
Acosta, Mario
Escribano, José M.
Parreño, Gladys
Wigdorovitz, Andres
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pestivirus de la Diarrea Bovina
Vacuna
Respuesta Inmunológica
Ganado Bovino
Cobaya
Bovine Diarrhoea Pestivirus
Vaccines
Immune Response
Cattle
Guinea Pigs
BVDV
topic Pestivirus de la Diarrea Bovina
Vacuna
Respuesta Inmunológica
Ganado Bovino
Cobaya
Bovine Diarrhoea Pestivirus
Vaccines
Immune Response
Cattle
Guinea Pigs
BVDV
dc.description.none.fl_txt_mv Bovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two primary methods used to control the spread of BVDV: the elimination of persistently infected (PI) animals and vaccination. Currently, modified live or inactivated vaccines are used in BVDV vaccination programmes, but there are safety risks or insufficient protection, respectively, with these vaccines. Here, we report the development and efficacy of the first targeted subunit vaccine against BVDV. The core of the vaccine is the fusion of the BVDV structural protein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present on antigen-presenting cells. Industrial production of the vaccine is carried out using the baculovirus expression vector system (BEVS) using single-use manufacturing technologies. This new subunit vaccine induces strong BVDV-specific neutralizing antibodies in guinea pigs and cattle. Importantly, in cattle with low levels of natural BVDV-specific neutralizing antibodies, the vaccine induced strong neutralizing antibody levels to above the protective threshold, as determined by a competition ELISA. The APCH-E2 vaccine induced a rapid and sustained neutralizing antibody response compared with a conventional vaccine in cattle.
Instituto de Virologia
Fil: Bellido, Demian. Vetanco SA; Argentina
Fil: Bellido, Demian. Bioinnovo SA; Argentina
Fil: Baztarrica, Josefina. Vetanco SA; Argentina
Fil: Baztarrica, Josefina. Bioinnovo SA; Argentina
Fil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Rocha, Lucía Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Pecora, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acosta, Mario. Vetanco SA; Argentina
Fil: Escribano, José M. Algenex; España
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Parreño, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parreño, Viviana. Bioinnovo S.A.; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina
Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Wigdorovitz, Andres. Bioinnovo S.A.; Argentina
description Bovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two primary methods used to control the spread of BVDV: the elimination of persistently infected (PI) animals and vaccination. Currently, modified live or inactivated vaccines are used in BVDV vaccination programmes, but there are safety risks or insufficient protection, respectively, with these vaccines. Here, we report the development and efficacy of the first targeted subunit vaccine against BVDV. The core of the vaccine is the fusion of the BVDV structural protein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present on antigen-presenting cells. Industrial production of the vaccine is carried out using the baculovirus expression vector system (BEVS) using single-use manufacturing technologies. This new subunit vaccine induces strong BVDV-specific neutralizing antibodies in guinea pigs and cattle. Importantly, in cattle with low levels of natural BVDV-specific neutralizing antibodies, the vaccine induced strong neutralizing antibody levels to above the protective threshold, as determined by a competition ELISA. The APCH-E2 vaccine induced a rapid and sustained neutralizing antibody response compared with a conventional vaccine in cattle.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
2023-01-13T13:50:39Z
2023-01-13T13:50:39Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13907
https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13952
1865-1674
1865-1682
https://doi.org/10.1111/tbed.13952
url http://hdl.handle.net/20.500.12123/13907
https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13952
https://doi.org/10.1111/tbed.13952
identifier_str_mv 1865-1674
1865-1682
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Transboundary and Emerging Diseases 68 (6) : 3474-3481 (November 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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