Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2
- Autores
- Pecora, Andrea; Malacari, Darío Amilcar; Pérez Aguirreburualde, María Sol; Bellido, Demian; Nuñez, Maria del Carmen; Dus Santos, Maria Jose; Martínez Escribano, José Angel; Wigdorovitz, Andres
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in insect cells by a baculovirus vector as fusion proteins with a single chain antibody, named APCH I, which recognizes the β-chain of the MHC Class II antigen. The three chimeric proteins were evaluated for their immunogenicity in a guinea pig model as well as in colostrum-deprived calves. Once the immune response in experimentally vaccinated calves was evaluated, immunized animals were challenged with type 1b or type 2b BVDV in order to study the protection conferred by the experimental vaccine. The recombinant APCH I-tE21a-1b-2a vaccine was immunogenic both in guinea pigs and calves, inducing neutralizing antibodies. After BVDV type 1b and type 2 challenge of vaccinated calves in a proof of concept, the type 1b virus could not be isolated in any animal; meanwhile it was detected in all challenged non-vaccinated control animals. However, the type 2 BVDV was isolated to a lesser extent compared to unvaccinated animals challenged with type 2 BVDV. Clinical signs associated to BVDV, hyperthermia and leukopenia were reduced with respect to controls in all vaccinated calves. Given these results, this multivalent vaccine holds promise for a safe and effective tool to control BVDV in herds
Instituto de Virología
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Bellido, Demian. Vetanco SA; Argentina
Fil: Nuñez, Maria del Carmen. Algenex SL, Madrid; España
Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Martínez Escribano, José Angel. Instituto Nacional de Investigación Agraria. Departamento de Biotecnología; España
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Vaccine 33 (39) : 5163-5171. (22 September 2015)
- Materia
-
Pestivirus de la Diarrea Bovina
Bovine Diarrhoea Pestivirus
Glycoproteins
Glicoproteínas
Polyvalent Vaccines
Vacuna Polivalente
Vaccines
Vacuna
E2 Glycoprotein
BVDV - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4536
Ver los metadatos del registro completo
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Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2Pecora, AndreaMalacari, Darío AmilcarPérez Aguirreburualde, María SolBellido, DemianNuñez, Maria del CarmenDus Santos, Maria JoseMartínez Escribano, José AngelWigdorovitz, AndresPestivirus de la Diarrea BovinaBovine Diarrhoea PestivirusGlycoproteinsGlicoproteínasPolyvalent VaccinesVacuna PolivalenteVaccinesVacunaE2 GlycoproteinBVDVThe aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in insect cells by a baculovirus vector as fusion proteins with a single chain antibody, named APCH I, which recognizes the β-chain of the MHC Class II antigen. The three chimeric proteins were evaluated for their immunogenicity in a guinea pig model as well as in colostrum-deprived calves. Once the immune response in experimentally vaccinated calves was evaluated, immunized animals were challenged with type 1b or type 2b BVDV in order to study the protection conferred by the experimental vaccine. The recombinant APCH I-tE21a-1b-2a vaccine was immunogenic both in guinea pigs and calves, inducing neutralizing antibodies. After BVDV type 1b and type 2 challenge of vaccinated calves in a proof of concept, the type 1b virus could not be isolated in any animal; meanwhile it was detected in all challenged non-vaccinated control animals. However, the type 2 BVDV was isolated to a lesser extent compared to unvaccinated animals challenged with type 2 BVDV. Clinical signs associated to BVDV, hyperthermia and leukopenia were reduced with respect to controls in all vaccinated calves. Given these results, this multivalent vaccine holds promise for a safe and effective tool to control BVDV in herdsInstituto de VirologíaFil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Bellido, Demian. Vetanco SA; ArgentinaFil: Nuñez, Maria del Carmen. Algenex SL, Madrid; EspañaFil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Martínez Escribano, José Angel. Instituto Nacional de Investigación Agraria. Departamento de Biotecnología; EspañaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-03-01T18:56:08Z2019-03-01T18:56:08Z2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4536https://www.sciencedirect.com/science/article/pii/S0264410X15011147?via%3Dihub0264-410Xhttps://doi.org/10.1016/j.vaccine.2015.07.106Vaccine 33 (39) : 5163-5171. (22 September 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:50Zoai:localhost:20.500.12123/4536instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:51.373INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| spellingShingle |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 Pecora, Andrea Pestivirus de la Diarrea Bovina Bovine Diarrhoea Pestivirus Glycoproteins Glicoproteínas Polyvalent Vaccines Vacuna Polivalente Vaccines Vacuna E2 Glycoprotein BVDV |
| title_short |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_full |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_fullStr |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_full_unstemmed |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_sort |
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| dc.creator.none.fl_str_mv |
Pecora, Andrea Malacari, Darío Amilcar Pérez Aguirreburualde, María Sol Bellido, Demian Nuñez, Maria del Carmen Dus Santos, Maria Jose Martínez Escribano, José Angel Wigdorovitz, Andres |
| author |
Pecora, Andrea |
| author_facet |
Pecora, Andrea Malacari, Darío Amilcar Pérez Aguirreburualde, María Sol Bellido, Demian Nuñez, Maria del Carmen Dus Santos, Maria Jose Martínez Escribano, José Angel Wigdorovitz, Andres |
| author_role |
author |
| author2 |
Malacari, Darío Amilcar Pérez Aguirreburualde, María Sol Bellido, Demian Nuñez, Maria del Carmen Dus Santos, Maria Jose Martínez Escribano, José Angel Wigdorovitz, Andres |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Pestivirus de la Diarrea Bovina Bovine Diarrhoea Pestivirus Glycoproteins Glicoproteínas Polyvalent Vaccines Vacuna Polivalente Vaccines Vacuna E2 Glycoprotein BVDV |
| topic |
Pestivirus de la Diarrea Bovina Bovine Diarrhoea Pestivirus Glycoproteins Glicoproteínas Polyvalent Vaccines Vacuna Polivalente Vaccines Vacuna E2 Glycoprotein BVDV |
| dc.description.none.fl_txt_mv |
The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in insect cells by a baculovirus vector as fusion proteins with a single chain antibody, named APCH I, which recognizes the β-chain of the MHC Class II antigen. The three chimeric proteins were evaluated for their immunogenicity in a guinea pig model as well as in colostrum-deprived calves. Once the immune response in experimentally vaccinated calves was evaluated, immunized animals were challenged with type 1b or type 2b BVDV in order to study the protection conferred by the experimental vaccine. The recombinant APCH I-tE21a-1b-2a vaccine was immunogenic both in guinea pigs and calves, inducing neutralizing antibodies. After BVDV type 1b and type 2 challenge of vaccinated calves in a proof of concept, the type 1b virus could not be isolated in any animal; meanwhile it was detected in all challenged non-vaccinated control animals. However, the type 2 BVDV was isolated to a lesser extent compared to unvaccinated animals challenged with type 2 BVDV. Clinical signs associated to BVDV, hyperthermia and leukopenia were reduced with respect to controls in all vaccinated calves. Given these results, this multivalent vaccine holds promise for a safe and effective tool to control BVDV in herds Instituto de Virología Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Bellido, Demian. Vetanco SA; Argentina Fil: Nuñez, Maria del Carmen. Algenex SL, Madrid; España Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Martínez Escribano, José Angel. Instituto Nacional de Investigación Agraria. Departamento de Biotecnología; España Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in insect cells by a baculovirus vector as fusion proteins with a single chain antibody, named APCH I, which recognizes the β-chain of the MHC Class II antigen. The three chimeric proteins were evaluated for their immunogenicity in a guinea pig model as well as in colostrum-deprived calves. Once the immune response in experimentally vaccinated calves was evaluated, immunized animals were challenged with type 1b or type 2b BVDV in order to study the protection conferred by the experimental vaccine. The recombinant APCH I-tE21a-1b-2a vaccine was immunogenic both in guinea pigs and calves, inducing neutralizing antibodies. After BVDV type 1b and type 2 challenge of vaccinated calves in a proof of concept, the type 1b virus could not be isolated in any animal; meanwhile it was detected in all challenged non-vaccinated control animals. However, the type 2 BVDV was isolated to a lesser extent compared to unvaccinated animals challenged with type 2 BVDV. Clinical signs associated to BVDV, hyperthermia and leukopenia were reduced with respect to controls in all vaccinated calves. Given these results, this multivalent vaccine holds promise for a safe and effective tool to control BVDV in herds |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-09 2019-03-01T18:56:08Z 2019-03-01T18:56:08Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/4536 https://www.sciencedirect.com/science/article/pii/S0264410X15011147?via%3Dihub 0264-410X https://doi.org/10.1016/j.vaccine.2015.07.106 |
| url |
http://hdl.handle.net/20.500.12123/4536 https://www.sciencedirect.com/science/article/pii/S0264410X15011147?via%3Dihub https://doi.org/10.1016/j.vaccine.2015.07.106 |
| identifier_str_mv |
0264-410X |
| dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/restrictedAccess |
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restrictedAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
Vaccine 33 (39) : 5163-5171. (22 September 2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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