Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption
- Autores
- Burrels, Alison; Benavides-Silván, Julio; Canton, German Jose; Garcia, João L.; Bartley, Paul M.; Nath, Mintu; Thomson, Jackie; Chianini, Francesca; Innes, Elisabeth A.; Katze, Frank
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safety
Fil: Burrels, Alison. Moredun Research Institute. Pentlands Science Park; Reino Unido
Fil: Benavides-Silván, Julio. Moredun Research Institute. Pentlands Science Park; Reino Unido. Instituto de Ganadería de Montaña; España
Fil: Canton, German José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Moredun Research Institute. Pentlands Science Park; Reino Unido
Fil: Garcia, João L. Moredun Research Institute. Pentlands Science Park; Reino Unido. Universidade Estadual de Londrina. Departamento de Medicina Veterinária Preventiva; Brasil
Fil: Bartley, Paul M. Moredun Research Institute. Pentlands Science Park; Reino Unido
Fil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino Unido
Fil: Thomson, Jackie. Moredun Research Institute. Pentlands Science Park; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute. Pentlands Science Park; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute. Pentlands Science Park; Reino Unido
Fil: Katze, Frank. Moredun Research Institute. Pentlands Science Park; Reino Unido - Fuente
- Veterinary research 46 (1) : 1-12. (2015)
- Materia
-
Vacunación
Vaccination
Swine
Animal Diseases
Pork
Cerdo
Toxoplasma Gondii
Enfermedades de los Animales
Carne de Cerdo
S48 Strain
Cepa S48 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/1151
Ver los metadatos del registro completo
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Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumptionBurrels, AlisonBenavides-Silván, JulioCanton, German JoseGarcia, João L.Bartley, Paul M.Nath, MintuThomson, JackieChianini, FrancescaInnes, Elisabeth A.Katze, FrankVacunaciónVaccinationSwineAnimal DiseasesPorkCerdoToxoplasma GondiiEnfermedades de los AnimalesCarne de CerdoS48 StrainCepa S48As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safetyFil: Burrels, Alison. Moredun Research Institute. Pentlands Science Park; Reino UnidoFil: Benavides-Silván, Julio. Moredun Research Institute. Pentlands Science Park; Reino Unido. Instituto de Ganadería de Montaña; EspañaFil: Canton, German José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Moredun Research Institute. Pentlands Science Park; Reino UnidoFil: Garcia, João L. Moredun Research Institute. Pentlands Science Park; Reino Unido. Universidade Estadual de Londrina. Departamento de Medicina Veterinária Preventiva; BrasilFil: Bartley, Paul M. Moredun Research Institute. Pentlands Science Park; Reino UnidoFil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino UnidoFil: Thomson, Jackie. Moredun Research Institute. Pentlands Science Park; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute. Pentlands Science Park; Reino UnidoFil: Innes, Elisabeth A. Moredun Research Institute. Pentlands Science Park; Reino UnidoFil: Katze, Frank. Moredun Research Institute. Pentlands Science Park; Reino Unido2017-09-06T15:05:29Z2017-09-06T15:05:29Z2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1151https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0177-01297-9716https://doi.org/10.1186/s13567-015-0177-0Veterinary research 46 (1) : 1-12. (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:46:59Zoai:localhost:20.500.12123/1151instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:00.442INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| spellingShingle |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption Burrels, Alison Vacunación Vaccination Swine Animal Diseases Pork Cerdo Toxoplasma Gondii Enfermedades de los Animales Carne de Cerdo S48 Strain Cepa S48 |
| title_short |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_full |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_fullStr |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_full_unstemmed |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_sort |
Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| dc.creator.none.fl_str_mv |
Burrels, Alison Benavides-Silván, Julio Canton, German Jose Garcia, João L. Bartley, Paul M. Nath, Mintu Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Katze, Frank |
| author |
Burrels, Alison |
| author_facet |
Burrels, Alison Benavides-Silván, Julio Canton, German Jose Garcia, João L. Bartley, Paul M. Nath, Mintu Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Katze, Frank |
| author_role |
author |
| author2 |
Benavides-Silván, Julio Canton, German Jose Garcia, João L. Bartley, Paul M. Nath, Mintu Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Katze, Frank |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Vacunación Vaccination Swine Animal Diseases Pork Cerdo Toxoplasma Gondii Enfermedades de los Animales Carne de Cerdo S48 Strain Cepa S48 |
| topic |
Vacunación Vaccination Swine Animal Diseases Pork Cerdo Toxoplasma Gondii Enfermedades de los Animales Carne de Cerdo S48 Strain Cepa S48 |
| dc.description.none.fl_txt_mv |
As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safety Fil: Burrels, Alison. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Benavides-Silván, Julio. Moredun Research Institute. Pentlands Science Park; Reino Unido. Instituto de Ganadería de Montaña; España Fil: Canton, German José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Garcia, João L. Moredun Research Institute. Pentlands Science Park; Reino Unido. Universidade Estadual de Londrina. Departamento de Medicina Veterinária Preventiva; Brasil Fil: Bartley, Paul M. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino Unido Fil: Thomson, Jackie. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Katze, Frank. Moredun Research Institute. Pentlands Science Park; Reino Unido |
| description |
As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safety |
| publishDate |
2015 |
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2015 2017-09-06T15:05:29Z 2017-09-06T15:05:29Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/1151 https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0177-0 1297-9716 https://doi.org/10.1186/s13567-015-0177-0 |
| url |
http://hdl.handle.net/20.500.12123/1151 https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0177-0 https://doi.org/10.1186/s13567-015-0177-0 |
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1297-9716 |
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eng |
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eng |
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