On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling
- Autores
- Farías, Silvia Sara; Londonio, Juan Agustín; Quintero, César Eugenio; Befani, Romina; Soro, Enrique Mariano; Smichowski, Patricia Nora
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average.
EEA Concepción del Uruguay
Fil: Farías, Silvia Sara. Comisión Nacional de Energía Atómica; Argentina
Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Soro, Enrique Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Microchemical journal 120 : 34–39. (2015)
- Materia
-
Arroz
Oryza Sativa
Arsénico
HPLC
Toxicidad
Toxicity
Arsenic
Rice
HPLC–HG–AFS
Mild Extraction
Speciation Analysis of As
Argentina - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2824
Ver los metadatos del registro completo
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On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS couplingFarías, Silvia SaraLondonio, Juan AgustínQuintero, César EugenioBefani, RominaSoro, Enrique MarianoSmichowski, Patricia NoraArrozOryza SativaArsénicoHPLCToxicidadToxicityArsenicRiceHPLC–HG–AFSMild ExtractionSpeciation Analysis of AsArgentinaAn investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average.EEA Concepción del UruguayFil: Farías, Silvia Sara. Comisión Nacional de Energía Atómica; ArgentinaFil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Soro, Enrique Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-07-19T13:22:37Z2018-07-19T13:22:37Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/28240026-265Xhttps://doi.org/10.1016/j.microc.2014.12.010Microchemical journal 120 : 34–39. (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:21Zoai:localhost:20.500.12123/2824instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:22.276INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| spellingShingle |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling Farías, Silvia Sara Arroz Oryza Sativa Arsénico HPLC Toxicidad Toxicity Arsenic Rice HPLC–HG–AFS Mild Extraction Speciation Analysis of As Argentina |
| title_short |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_full |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_fullStr |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_full_unstemmed |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_sort |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| dc.creator.none.fl_str_mv |
Farías, Silvia Sara Londonio, Juan Agustín Quintero, César Eugenio Befani, Romina Soro, Enrique Mariano Smichowski, Patricia Nora |
| author |
Farías, Silvia Sara |
| author_facet |
Farías, Silvia Sara Londonio, Juan Agustín Quintero, César Eugenio Befani, Romina Soro, Enrique Mariano Smichowski, Patricia Nora |
| author_role |
author |
| author2 |
Londonio, Juan Agustín Quintero, César Eugenio Befani, Romina Soro, Enrique Mariano Smichowski, Patricia Nora |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Arroz Oryza Sativa Arsénico HPLC Toxicidad Toxicity Arsenic Rice HPLC–HG–AFS Mild Extraction Speciation Analysis of As Argentina |
| topic |
Arroz Oryza Sativa Arsénico HPLC Toxicidad Toxicity Arsenic Rice HPLC–HG–AFS Mild Extraction Speciation Analysis of As Argentina |
| dc.description.none.fl_txt_mv |
An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average. EEA Concepción del Uruguay Fil: Farías, Silvia Sara. Comisión Nacional de Energía Atómica; Argentina Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina Fil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina Fil: Soro, Enrique Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average. |
| publishDate |
2015 |
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2015 2018-07-19T13:22:37Z 2018-07-19T13:22:37Z |
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http://hdl.handle.net/20.500.12123/2824 0026-265X https://doi.org/10.1016/j.microc.2014.12.010 |
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http://hdl.handle.net/20.500.12123/2824 https://doi.org/10.1016/j.microc.2014.12.010 |
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Microchemical journal 120 : 34–39. (2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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