On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling
- Autores
- Farías, Silvia S.; Londonio, Juan Agustín; Quintero, Cesar Eugenio; Befani, Romina; Soro, Mariano; Smichowski, Patricia Nora
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)-hydride generation (HG)-atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103±2°C and mild conditions were adopted for species extraction (0.28molL-1 HNO3 at 95±3°C for 90min). Method validation included, evaluation of linearity; limits of detection, 0.020μgg-1 for As(III) and 0.025μgg-1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39mgkg-1. The mean concentrations of sum of the As species extracted and determined by HPLC-HG-AFS was approximately 99.7% of certified value for total As in NIST SRM "Rice flour". Levels of the four species in the analyzed samples were in the order DMA>As(III)>As(V)>MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average.
Fil: Farías, Silvia S.. Comisión Nacional de Energía Atómica; Argentina
Fil: Londonio, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina
Fil: Soro, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Hplc-Hg-Afs
Method Validation
Mild Extraction
Rice
Speciation Analysis of As - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/37485
Ver los metadatos del registro completo
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On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS couplingFarías, Silvia S.Londonio, Juan AgustínQuintero, Cesar EugenioBefani, RominaSoro, MarianoSmichowski, Patricia NoraHplc-Hg-AfsMethod ValidationMild ExtractionRiceSpeciation Analysis of Ashttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)-hydride generation (HG)-atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103±2°C and mild conditions were adopted for species extraction (0.28molL-1 HNO3 at 95±3°C for 90min). Method validation included, evaluation of linearity; limits of detection, 0.020μgg-1 for As(III) and 0.025μgg-1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39mgkg-1. The mean concentrations of sum of the As species extracted and determined by HPLC-HG-AFS was approximately 99.7% of certified value for total As in NIST SRM "Rice flour". Levels of the four species in the analyzed samples were in the order DMA>As(III)>As(V)>MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average.Fil: Farías, Silvia S.. Comisión Nacional de Energía Atómica; ArgentinaFil: Londonio, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Soro, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37485Farías, Silvia S.; Londonio, Juan Agustín; Quintero, Cesar Eugenio; Befani, Romina; Soro, Mariano; et al.; On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling; Elsevier Science; Microchemical Journal; 120; 5-2015; 34-390026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2014.12.010info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X14002409info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:27Zoai:ri.conicet.gov.ar:11336/37485instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:27.696CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling |
| title |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling |
| spellingShingle |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling Farías, Silvia S. Hplc-Hg-Afs Method Validation Mild Extraction Rice Speciation Analysis of As |
| title_short |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling |
| title_full |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling |
| title_fullStr |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling |
| title_full_unstemmed |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling |
| title_sort |
On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling |
| dc.creator.none.fl_str_mv |
Farías, Silvia S. Londonio, Juan Agustín Quintero, Cesar Eugenio Befani, Romina Soro, Mariano Smichowski, Patricia Nora |
| author |
Farías, Silvia S. |
| author_facet |
Farías, Silvia S. Londonio, Juan Agustín Quintero, Cesar Eugenio Befani, Romina Soro, Mariano Smichowski, Patricia Nora |
| author_role |
author |
| author2 |
Londonio, Juan Agustín Quintero, Cesar Eugenio Befani, Romina Soro, Mariano Smichowski, Patricia Nora |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Hplc-Hg-Afs Method Validation Mild Extraction Rice Speciation Analysis of As |
| topic |
Hplc-Hg-Afs Method Validation Mild Extraction Rice Speciation Analysis of As |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)-hydride generation (HG)-atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103±2°C and mild conditions were adopted for species extraction (0.28molL-1 HNO3 at 95±3°C for 90min). Method validation included, evaluation of linearity; limits of detection, 0.020μgg-1 for As(III) and 0.025μgg-1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39mgkg-1. The mean concentrations of sum of the As species extracted and determined by HPLC-HG-AFS was approximately 99.7% of certified value for total As in NIST SRM "Rice flour". Levels of the four species in the analyzed samples were in the order DMA>As(III)>As(V)>MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average. Fil: Farías, Silvia S.. Comisión Nacional de Energía Atómica; Argentina Fil: Londonio, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina Fil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina Fil: Soro, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)-hydride generation (HG)-atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103±2°C and mild conditions were adopted for species extraction (0.28molL-1 HNO3 at 95±3°C for 90min). Method validation included, evaluation of linearity; limits of detection, 0.020μgg-1 for As(III) and 0.025μgg-1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39mgkg-1. The mean concentrations of sum of the As species extracted and determined by HPLC-HG-AFS was approximately 99.7% of certified value for total As in NIST SRM "Rice flour". Levels of the four species in the analyzed samples were in the order DMA>As(III)>As(V)>MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-05 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/37485 Farías, Silvia S.; Londonio, Juan Agustín; Quintero, Cesar Eugenio; Befani, Romina; Soro, Mariano; et al.; On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling; Elsevier Science; Microchemical Journal; 120; 5-2015; 34-39 0026-265X CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/37485 |
| identifier_str_mv |
Farías, Silvia S.; Londonio, Juan Agustín; Quintero, Cesar Eugenio; Befani, Romina; Soro, Mariano; et al.; On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling; Elsevier Science; Microchemical Journal; 120; 5-2015; 34-39 0026-265X CONICET Digital CONICET |
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eng |
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eng |
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