Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues

Autores
Canton, German Jose; Bartley, Paul M.; Bartley, Kathryn; Todd, Helen; Chianini, Francesca; Katzer, Frank
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues.
EEA Balcarce
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Bartley, Kathryn. Moredun Research Institute; Reino Unido
Fil: Todd, Helen. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fuente
Veterinary Immunology and Immunopathology 151 (3–4) : 342-347 (February 2013)
Materia
Rumiante
Citoquinas
Hibridación
Respuesta Inmunológica
Análisis de Tejidos
Ruminants
Cytokines
Hybridization
Immune Response
Tissue Analysis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4327
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4327
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissuesCanton, German JoseBartley, Paul M.Bartley, KathrynTodd, HelenChianini, FrancescaKatzer, FrankRumianteCitoquinasHibridaciónRespuesta InmunológicaAnálisis de TejidosRuminantsCytokinesHybridizationImmune ResponseTissue AnalysisPro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues.EEA BalcarceFil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Bartley, Paul M. Moredun Research Institute; Reino UnidoFil: Bartley, Kathryn. Moredun Research Institute; Reino UnidoFil: Todd, Helen. Moredun Research Institute; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute; Reino UnidoFil: Katzer, Frank. Moredun Research Institute; Reino UnidoElsevier2019-01-24T14:34:21Z2019-01-24T14:34:21Z2013-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0165242712004242http://hdl.handle.net/20.500.12123/43270165-24271873-2534https://doi.org/10.1016/j.vetimm.2012.11.019Veterinary Immunology and Immunopathology 151 (3–4) : 342-347 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4327instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.12INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
title Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
spellingShingle Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
Canton, German Jose
Rumiante
Citoquinas
Hibridación
Respuesta Inmunológica
Análisis de Tejidos
Ruminants
Cytokines
Hybridization
Immune Response
Tissue Analysis
title_short Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
title_full Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
title_fullStr Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
title_full_unstemmed Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
title_sort Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
dc.creator.none.fl_str_mv Canton, German Jose
Bartley, Paul M.
Bartley, Kathryn
Todd, Helen
Chianini, Francesca
Katzer, Frank
author Canton, German Jose
author_facet Canton, German Jose
Bartley, Paul M.
Bartley, Kathryn
Todd, Helen
Chianini, Francesca
Katzer, Frank
author_role author
author2 Bartley, Paul M.
Bartley, Kathryn
Todd, Helen
Chianini, Francesca
Katzer, Frank
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Rumiante
Citoquinas
Hibridación
Respuesta Inmunológica
Análisis de Tejidos
Ruminants
Cytokines
Hybridization
Immune Response
Tissue Analysis
topic Rumiante
Citoquinas
Hibridación
Respuesta Inmunológica
Análisis de Tejidos
Ruminants
Cytokines
Hybridization
Immune Response
Tissue Analysis
dc.description.none.fl_txt_mv Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues.
EEA Balcarce
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Bartley, Kathryn. Moredun Research Institute; Reino Unido
Fil: Todd, Helen. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
description Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-15
2019-01-24T14:34:21Z
2019-01-24T14:34:21Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0165242712004242
http://hdl.handle.net/20.500.12123/4327
0165-2427
1873-2534
https://doi.org/10.1016/j.vetimm.2012.11.019
url https://www.sciencedirect.com/science/article/pii/S0165242712004242
http://hdl.handle.net/20.500.12123/4327
https://doi.org/10.1016/j.vetimm.2012.11.019
identifier_str_mv 0165-2427
1873-2534
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 151 (3–4) : 342-347 (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619130159759360
score 12.559606

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