Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol produc...

Autores: Montiel, Carmina; Hernández-Meléndez, Oscar; Marques, Susana; Gírio, Francisco; Tavares, João; Ontañon, Ornella Mailen; Campos, Eleonora; Bárzana, Eduardo
Año de publicación: 2024
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymatic hydrolysis of pretreated blue agave bagasse (BAB) at the high solids load of 20% (w/v). Three different combinations of cellulase and xylanases were evaluated. When Cellic® CTec2 was used at a dosage of 10 FPU/g oven-dried solids (ODS) supplemented with XynA or MaPYCC 5535T at an endo-xylanase dosage of 100 U/g ODS, increases in the xylose yield of 30% and 33%, respectively, were obtained. When applying in-house xylanases alone (at an endo-xylanase dosage of 100 U/g ODS), xylan in BAB was selectively hydrolyzed into xylose with 5% yield with MaPYCC 5535T, while no xylose was detected with XynA. Interestingly, a synergic effect of Cellic® CTec 2 with both xylanases was observed when using a low dosage of 1 FPU/g ODS (allowing for some liquefaction of the reaction mixture), promoting xylose and glucose release by either xylanase. A higher concentration of monomeric sugars was obtained with 10 FPU/g ODS of Cellic® Ctec 2 supplemented with 100 U/g ODS of MaPYCC 5535T, followed by XynA. The improvement in saccharification through the synergistic combination of in-house xylanases and commercial cellulases allows for the obtention of sugar-rich hydrolysates, which enhances the technical sustainability of the process. Hydrolysates were then fermented using recombinant Cellux 4TM yeast to yield 45 g/L ethanol, representing an increase of about 30% with respect to the control obtained with only the commercial cellulase cocktail. The surface modification of agave biomass with the different combinations of enzymes was evidenced by scanning electron microscopy (SEM).
Instituto de Biotecnología
Fil: Montiel, Carmina. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; México
Fil: Hernández-Meléndez, Oscar. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Ingeniería Química; México
Fil: Marques, Susana. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal
Fil: Gírio, Francisco. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal
Fil: Tavares, João. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal
Fil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bárzana, Eduardo. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; México
Fuente: Sustainability 16 (16) : 6722 (Agosto 2024)
Materia: Agave
Hidrólisis Enzimática
Fermentación
Celulasa
Bioetanol
Enzymatic Hydrolysis
Fermentation
Cellulase
Bioethanol
Xylanase Enzymatic
Xilanasa Enzimática
Nivel de accesibilidad: acceso abierto
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/19057

Acceder

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spelling	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol productionMontiel, CarminaHernández-Meléndez, OscarMarques, SusanaGírio, FranciscoTavares, JoãoOntañon, Ornella MailenCampos, EleonoraBárzana, EduardoAgaveHidrólisis EnzimáticaFermentaciónCelulasaBioetanolEnzymatic HydrolysisFermentationCellulaseBioethanolXylanase EnzymaticXilanasa EnzimáticaThe study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymatic hydrolysis of pretreated blue agave bagasse (BAB) at the high solids load of 20% (w/v). Three different combinations of cellulase and xylanases were evaluated. When Cellic® CTec2 was used at a dosage of 10 FPU/g oven-dried solids (ODS) supplemented with XynA or MaPYCC 5535T at an endo-xylanase dosage of 100 U/g ODS, increases in the xylose yield of 30% and 33%, respectively, were obtained. When applying in-house xylanases alone (at an endo-xylanase dosage of 100 U/g ODS), xylan in BAB was selectively hydrolyzed into xylose with 5% yield with MaPYCC 5535T, while no xylose was detected with XynA. Interestingly, a synergic effect of Cellic® CTec 2 with both xylanases was observed when using a low dosage of 1 FPU/g ODS (allowing for some liquefaction of the reaction mixture), promoting xylose and glucose release by either xylanase. A higher concentration of monomeric sugars was obtained with 10 FPU/g ODS of Cellic® Ctec 2 supplemented with 100 U/g ODS of MaPYCC 5535T, followed by XynA. The improvement in saccharification through the synergistic combination of in-house xylanases and commercial cellulases allows for the obtention of sugar-rich hydrolysates, which enhances the technical sustainability of the process. Hydrolysates were then fermented using recombinant Cellux 4TM yeast to yield 45 g/L ethanol, representing an increase of about 30% with respect to the control obtained with only the commercial cellulase cocktail. The surface modification of agave biomass with the different combinations of enzymes was evidenced by scanning electron microscopy (SEM).Instituto de BiotecnologíaFil: Montiel, Carmina. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; MéxicoFil: Hernández-Meléndez, Oscar. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Ingeniería Química; MéxicoFil: Marques, Susana. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; PortugalFil: Gírio, Francisco. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; PortugalFil: Tavares, João. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; PortugalFil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bárzana, Eduardo. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; MéxicoMDPI2024-08-22T09:58:05Z2024-08-22T09:58:05Z2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/19057https://www.mdpi.com/2071-1050/16/16/67222071-1050https://doi.org/10.3390/su16166722Sustainability 16 (16) : 6722 (Agosto 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:34Zoai:localhost:20.500.12123/19057instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:35.336INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
title	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
spellingShingle	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production Montiel, Carmina Agave Hidrólisis Enzimática Fermentación Celulasa Bioetanol Enzymatic Hydrolysis Fermentation Cellulase Bioethanol Xylanase Enzymatic Xilanasa Enzimática
title_short	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
title_full	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
title_fullStr	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
title_full_unstemmed	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
title_sort	Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
dc.creator.none.fl_str_mv	Montiel, Carmina Hernández-Meléndez, Oscar Marques, Susana Gírio, Francisco Tavares, João Ontañon, Ornella Mailen Campos, Eleonora Bárzana, Eduardo
author	Montiel, Carmina
author_facet	Montiel, Carmina Hernández-Meléndez, Oscar Marques, Susana Gírio, Francisco Tavares, João Ontañon, Ornella Mailen Campos, Eleonora Bárzana, Eduardo
author_role	author
author2	Hernández-Meléndez, Oscar Marques, Susana Gírio, Francisco Tavares, João Ontañon, Ornella Mailen Campos, Eleonora Bárzana, Eduardo
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Agave Hidrólisis Enzimática Fermentación Celulasa Bioetanol Enzymatic Hydrolysis Fermentation Cellulase Bioethanol Xylanase Enzymatic Xilanasa Enzimática
topic	Agave Hidrólisis Enzimática Fermentación Celulasa Bioetanol Enzymatic Hydrolysis Fermentation Cellulase Bioethanol Xylanase Enzymatic Xilanasa Enzimática
dc.description.none.fl_txt_mv	The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymatic hydrolysis of pretreated blue agave bagasse (BAB) at the high solids load of 20% (w/v). Three different combinations of cellulase and xylanases were evaluated. When Cellic® CTec2 was used at a dosage of 10 FPU/g oven-dried solids (ODS) supplemented with XynA or MaPYCC 5535T at an endo-xylanase dosage of 100 U/g ODS, increases in the xylose yield of 30% and 33%, respectively, were obtained. When applying in-house xylanases alone (at an endo-xylanase dosage of 100 U/g ODS), xylan in BAB was selectively hydrolyzed into xylose with 5% yield with MaPYCC 5535T, while no xylose was detected with XynA. Interestingly, a synergic effect of Cellic® CTec 2 with both xylanases was observed when using a low dosage of 1 FPU/g ODS (allowing for some liquefaction of the reaction mixture), promoting xylose and glucose release by either xylanase. A higher concentration of monomeric sugars was obtained with 10 FPU/g ODS of Cellic® Ctec 2 supplemented with 100 U/g ODS of MaPYCC 5535T, followed by XynA. The improvement in saccharification through the synergistic combination of in-house xylanases and commercial cellulases allows for the obtention of sugar-rich hydrolysates, which enhances the technical sustainability of the process. Hydrolysates were then fermented using recombinant Cellux 4TM yeast to yield 45 g/L ethanol, representing an increase of about 30% with respect to the control obtained with only the commercial cellulase cocktail. The surface modification of agave biomass with the different combinations of enzymes was evidenced by scanning electron microscopy (SEM). Instituto de Biotecnología Fil: Montiel, Carmina. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; México Fil: Hernández-Meléndez, Oscar. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Ingeniería Química; México Fil: Marques, Susana. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal Fil: Gírio, Francisco. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal Fil: Tavares, João. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal Fil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bárzana, Eduardo. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; México
description	The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymatic hydrolysis of pretreated blue agave bagasse (BAB) at the high solids load of 20% (w/v). Three different combinations of cellulase and xylanases were evaluated. When Cellic® CTec2 was used at a dosage of 10 FPU/g oven-dried solids (ODS) supplemented with XynA or MaPYCC 5535T at an endo-xylanase dosage of 100 U/g ODS, increases in the xylose yield of 30% and 33%, respectively, were obtained. When applying in-house xylanases alone (at an endo-xylanase dosage of 100 U/g ODS), xylan in BAB was selectively hydrolyzed into xylose with 5% yield with MaPYCC 5535T, while no xylose was detected with XynA. Interestingly, a synergic effect of Cellic® CTec 2 with both xylanases was observed when using a low dosage of 1 FPU/g ODS (allowing for some liquefaction of the reaction mixture), promoting xylose and glucose release by either xylanase. A higher concentration of monomeric sugars was obtained with 10 FPU/g ODS of Cellic® Ctec 2 supplemented with 100 U/g ODS of MaPYCC 5535T, followed by XynA. The improvement in saccharification through the synergistic combination of in-house xylanases and commercial cellulases allows for the obtention of sugar-rich hydrolysates, which enhances the technical sustainability of the process. Hydrolysates were then fermented using recombinant Cellux 4TM yeast to yield 45 g/L ethanol, representing an increase of about 30% with respect to the control obtained with only the commercial cellulase cocktail. The surface modification of agave biomass with the different combinations of enzymes was evidenced by scanning electron microscopy (SEM).
publishDate	2024
dc.date.none.fl_str_mv	2024-08-22T09:58:05Z 2024-08-22T09:58:05Z 2024-08
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/19057 https://www.mdpi.com/2071-1050/16/16/6722 2071-1050 https://doi.org/10.3390/su16166722
url	http://hdl.handle.net/20.500.12123/19057 https://www.mdpi.com/2071-1050/16/16/6722 https://doi.org/10.3390/su16166722
identifier_str_mv	2071-1050
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	Sustainability 16 (16) : 6722 (Agosto 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
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repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol produc...

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