Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction

Autores
Ontañon, Ornella Mailen; Bedő, Soma; Ghio, Silvina; Garrido, Mercedes Maria; Topalian, Juliana; Jahola, Dóra; Fehér, Anikó; Valacco, Maria Pia; Campos, Eleonora; Fehér, Csaba
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain. Upscaling from flasks to fermentation in a bench scale bioreactor resulted in equivalent production of extracellular xylanolytic enzymatic extracts and freeze drying was a successful method for concentration and conservation of the extracellular enzymes, retaining 80% activity. Moreover, enzymatic cocktails composed of combined extra and intracellular extracts effectively hydrolysed the hemicellulose fraction of extruded barley straw into xylose and xylooligosaccharides.
Instituto de Biotecnología
Fil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ontañon, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bedő, Soma. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ghio, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garrido, Mercedes Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garrido, Mercedes Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular.
Fil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jahola, Dóra. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
Fil: Fehér, Anikó. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
Fil: Valacco, Maria Pia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina
Fil: Valacco, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fehér, Csaba. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
Fuente
Applied Microbiology and Biotechnology 105 : 4577-4588 (2021)
Materia
Cellulomonas
Lignocellulose
Enzymatic Hydrolysis
Biomass
Lignocelulosa
Hidrólisis Enzimática
Biomasa
Xilanasas
Xylanases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10779
Acceder
id INTADig_7348526ccdf80b601103c7b25fa63d21
oai_identifier_str oai:localhost:20.500.12123/10779
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstructionOntañon, Ornella MailenBedő, SomaGhio, SilvinaGarrido, Mercedes MariaTopalian, JulianaJahola, DóraFehér, AnikóValacco, Maria PiaCampos, EleonoraFehér, CsabaCellulomonasLignocelluloseEnzymatic HydrolysisBiomassLignocelulosaHidrólisis EnzimáticaBiomasaXilanasasXylanasesOne of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain. Upscaling from flasks to fermentation in a bench scale bioreactor resulted in equivalent production of extracellular xylanolytic enzymatic extracts and freeze drying was a successful method for concentration and conservation of the extracellular enzymes, retaining 80% activity. Moreover, enzymatic cocktails composed of combined extra and intracellular extracts effectively hydrolysed the hemicellulose fraction of extruded barley straw into xylose and xylooligosaccharides.Instituto de BiotecnologíaFil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ontañon, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bedő, Soma. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; HungríaFil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ghio, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garrido, Mercedes Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Garrido, Mercedes Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular.Fil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jahola, Dóra. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; HungríaFil: Fehér, Anikó. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; HungríaFil: Valacco, Maria Pia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; ArgentinaFil: Valacco, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fehér, Csaba. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; HungríaSpringer2021-11-15T16:19:12Z2021-11-15T16:19:12Z2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10779https://link.springer.com/article/10.1007%2Fs00253-021-11305-y0175-75981432-0614https://doi.org/10.1007/s00253-021-11305-yApplied Microbiology and Biotechnology 105 : 4577-4588 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:11Zoai:localhost:20.500.12123/10779instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:11.766INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
title Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
spellingShingle Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
Ontañon, Ornella Mailen
Cellulomonas
Lignocellulose
Enzymatic Hydrolysis
Biomass
Lignocelulosa
Hidrólisis Enzimática
Biomasa
Xilanasas
Xylanases
title_short Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
title_full Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
title_fullStr Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
title_full_unstemmed Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
title_sort Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
dc.creator.none.fl_str_mv Ontañon, Ornella Mailen
Bedő, Soma
Ghio, Silvina
Garrido, Mercedes Maria
Topalian, Juliana
Jahola, Dóra
Fehér, Anikó
Valacco, Maria Pia
Campos, Eleonora
Fehér, Csaba
author Ontañon, Ornella Mailen
author_facet Ontañon, Ornella Mailen
Bedő, Soma
Ghio, Silvina
Garrido, Mercedes Maria
Topalian, Juliana
Jahola, Dóra
Fehér, Anikó
Valacco, Maria Pia
Campos, Eleonora
Fehér, Csaba
author_role author
author2 Bedő, Soma
Ghio, Silvina
Garrido, Mercedes Maria
Topalian, Juliana
Jahola, Dóra
Fehér, Anikó
Valacco, Maria Pia
Campos, Eleonora
Fehér, Csaba
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cellulomonas
Lignocellulose
Enzymatic Hydrolysis
Biomass
Lignocelulosa
Hidrólisis Enzimática
Biomasa
Xilanasas
Xylanases
topic Cellulomonas
Lignocellulose
Enzymatic Hydrolysis
Biomass
Lignocelulosa
Hidrólisis Enzimática
Biomasa
Xilanasas
Xylanases
dc.description.none.fl_txt_mv One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain. Upscaling from flasks to fermentation in a bench scale bioreactor resulted in equivalent production of extracellular xylanolytic enzymatic extracts and freeze drying was a successful method for concentration and conservation of the extracellular enzymes, retaining 80% activity. Moreover, enzymatic cocktails composed of combined extra and intracellular extracts effectively hydrolysed the hemicellulose fraction of extruded barley straw into xylose and xylooligosaccharides.
Instituto de Biotecnología
Fil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ontañon, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bedő, Soma. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ghio, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garrido, Mercedes Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garrido, Mercedes Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular.
Fil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jahola, Dóra. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
Fil: Fehér, Anikó. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
Fil: Valacco, Maria Pia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina
Fil: Valacco, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fehér, Csaba. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría
description One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain. Upscaling from flasks to fermentation in a bench scale bioreactor resulted in equivalent production of extracellular xylanolytic enzymatic extracts and freeze drying was a successful method for concentration and conservation of the extracellular enzymes, retaining 80% activity. Moreover, enzymatic cocktails composed of combined extra and intracellular extracts effectively hydrolysed the hemicellulose fraction of extruded barley straw into xylose and xylooligosaccharides.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-15T16:19:12Z
2021-11-15T16:19:12Z
2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10779
https://link.springer.com/article/10.1007%2Fs00253-021-11305-y
0175-7598
1432-0614
https://doi.org/10.1007/s00253-021-11305-y
url http://hdl.handle.net/20.500.12123/10779
https://link.springer.com/article/10.1007%2Fs00253-021-11305-y
https://doi.org/10.1007/s00253-021-11305-y
identifier_str_mv 0175-7598
1432-0614
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Applied Microbiology and Biotechnology 105 : 4577-4588 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341392482828288
score 12.623145

Publicaciones similares