Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion

Autores
Coniglio, Romina O.; Fonseca, María I.; Diaz, Gabriela V.; Ontañon, Ornella Mailén; Ghio, Silvina; Campos, Eleonora; Zapata, Pedro Darío
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family.
Instituto de Biotecnología
Fil: Coniglio, Romina O. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, María I. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
Fil: Diaz, Gabriela V. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
Fuente
Arab Journal of Basic and Applied Sciences 26 (1) : 182-192 (Abril 2019)
Materia
Enzymatic Hydrolysis
Biomass
Hidrólisis Enzimática
Biomasa
Trametes
Enzimas Lignocelulíticas
Bioetanol
Lignocellulitic Enzymes
Bioethanol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5122
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5122
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network_name_str INTA Digital (INTA)
spelling Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversionConiglio, Romina O.Fonseca, María I.Diaz, Gabriela V.Ontañon, Ornella MailénGhio, SilvinaCampos, EleonoraZapata, Pedro DaríoEnzymatic HydrolysisBiomassHidrólisis EnzimáticaBiomasaTrametesEnzimas LignocelulíticasBioetanolLignocellulitic EnzymesBioethanolThe production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family.Instituto de BiotecnologíaFil: Coniglio, Romina O. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; ArgentinaFil: Fonseca, María I. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; ArgentinaFil: Diaz, Gabriela V. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; ArgentinaFil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; ArgentinaTaylor & Francis2019-05-16T10:57:40Z2019-05-16T10:57:40Z2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.tandfonline.com/doi/full/10.1080/25765299.2019.1598107http://hdl.handle.net/20.500.12123/51222576-5299https://doi.org/10.1080/25765299.2019.1598107Arab Journal of Basic and Applied Sciences 26 (1) : 182-192 (Abril 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:57Zoai:localhost:20.500.12123/5122instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:58.442INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
title Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
spellingShingle Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
Coniglio, Romina O.
Enzymatic Hydrolysis
Biomass
Hidrólisis Enzimática
Biomasa
Trametes
Enzimas Lignocelulíticas
Bioetanol
Lignocellulitic Enzymes
Bioethanol
title_short Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
title_full Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
title_fullStr Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
title_full_unstemmed Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
title_sort Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
dc.creator.none.fl_str_mv Coniglio, Romina O.
Fonseca, María I.
Diaz, Gabriela V.
Ontañon, Ornella Mailén
Ghio, Silvina
Campos, Eleonora
Zapata, Pedro Darío
author Coniglio, Romina O.
author_facet Coniglio, Romina O.
Fonseca, María I.
Diaz, Gabriela V.
Ontañon, Ornella Mailén
Ghio, Silvina
Campos, Eleonora
Zapata, Pedro Darío
author_role author
author2 Fonseca, María I.
Diaz, Gabriela V.
Ontañon, Ornella Mailén
Ghio, Silvina
Campos, Eleonora
Zapata, Pedro Darío
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Enzymatic Hydrolysis
Biomass
Hidrólisis Enzimática
Biomasa
Trametes
Enzimas Lignocelulíticas
Bioetanol
Lignocellulitic Enzymes
Bioethanol
topic Enzymatic Hydrolysis
Biomass
Hidrólisis Enzimática
Biomasa
Trametes
Enzimas Lignocelulíticas
Bioetanol
Lignocellulitic Enzymes
Bioethanol
dc.description.none.fl_txt_mv The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family.
Instituto de Biotecnología
Fil: Coniglio, Romina O. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, María I. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
Fil: Diaz, Gabriela V. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina
description The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family.
publishDate 2019
dc.date.none.fl_str_mv 2019-05-16T10:57:40Z
2019-05-16T10:57:40Z
2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1598107
http://hdl.handle.net/20.500.12123/5122
2576-5299
https://doi.org/10.1080/25765299.2019.1598107
url https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1598107
http://hdl.handle.net/20.500.12123/5122
https://doi.org/10.1080/25765299.2019.1598107
identifier_str_mv 2576-5299
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv Arab Journal of Basic and Applied Sciences 26 (1) : 182-192 (Abril 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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