DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality

Autores
Ynsaurralde Rivolta, Amanda Eugenia; Suvá, Mariana; Luchetti, Carolina Griselda; Bevacqua, Romina Jimena; Munilla, Sebastian; Rodriguez-Alvarez, Lleretny; Velasquez, Alejandra; Briski, Olinda; Lombardo, Daniel; Salamone, Daniel Felipe
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells.
EEA Mercedes
Fil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suvá, Mariana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Munilla, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina
Fil: Rodriguez-Alvarez, Lleretny. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Velasquez, Alejandra. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Briski, Olinda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Theriogenology 148 : 140-148 (May 2020)
Materia
Ganado Bovino
Suplementos
Estrés Oxidativo
Óvulo
Experimentación in Vitro
Cattle
Supplements
Oxidative Stress
Ova
In Vitro Experimentation
Oocytes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7380
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7380
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and qualityYnsaurralde Rivolta, Amanda EugeniaSuvá, MarianaLuchetti, Carolina GriseldaBevacqua, Romina JimenaMunilla, SebastianRodriguez-Alvarez, LleretnyVelasquez, AlejandraBriski, OlindaLombardo, DanielSalamone, Daniel FelipeGanado BovinoSuplementosEstrés OxidativoÓvuloExperimentación in VitroCattleSupplementsOxidative StressOvaIn Vitro ExperimentationOocytesThe molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells.EEA MercedesFil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Suvá, Mariana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Munilla, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; ArgentinaFil: Rodriguez-Alvarez, Lleretny. Universidad de Concepción. Facultad de Ciencias Veterinarias; ChileFil: Velasquez, Alejandra. Universidad de Concepción. Facultad de Ciencias Veterinarias; ChileFil: Briski, Olinda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina.Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2020-06-09T13:21:44Z2020-06-09T13:21:44Z2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7380https://www.sciencedirect.com/science/article/abs/pii/S0093691X203015760093-691X1879-3231https://doi.org/10.1016/j.theriogenology.2020.02.045Theriogenology 148 : 140-148 (May 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:49Zoai:localhost:20.500.12123/7380instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:49.895INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
title DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
spellingShingle DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
Ynsaurralde Rivolta, Amanda Eugenia
Ganado Bovino
Suplementos
Estrés Oxidativo
Óvulo
Experimentación in Vitro
Cattle
Supplements
Oxidative Stress
Ova
In Vitro Experimentation
Oocytes
title_short DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
title_full DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
title_fullStr DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
title_full_unstemmed DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
title_sort DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
dc.creator.none.fl_str_mv Ynsaurralde Rivolta, Amanda Eugenia
Suvá, Mariana
Luchetti, Carolina Griselda
Bevacqua, Romina Jimena
Munilla, Sebastian
Rodriguez-Alvarez, Lleretny
Velasquez, Alejandra
Briski, Olinda
Lombardo, Daniel
Salamone, Daniel Felipe
author Ynsaurralde Rivolta, Amanda Eugenia
author_facet Ynsaurralde Rivolta, Amanda Eugenia
Suvá, Mariana
Luchetti, Carolina Griselda
Bevacqua, Romina Jimena
Munilla, Sebastian
Rodriguez-Alvarez, Lleretny
Velasquez, Alejandra
Briski, Olinda
Lombardo, Daniel
Salamone, Daniel Felipe
author_role author
author2 Suvá, Mariana
Luchetti, Carolina Griselda
Bevacqua, Romina Jimena
Munilla, Sebastian
Rodriguez-Alvarez, Lleretny
Velasquez, Alejandra
Briski, Olinda
Lombardo, Daniel
Salamone, Daniel Felipe
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Suplementos
Estrés Oxidativo
Óvulo
Experimentación in Vitro
Cattle
Supplements
Oxidative Stress
Ova
In Vitro Experimentation
Oocytes
topic Ganado Bovino
Suplementos
Estrés Oxidativo
Óvulo
Experimentación in Vitro
Cattle
Supplements
Oxidative Stress
Ova
In Vitro Experimentation
Oocytes
dc.description.none.fl_txt_mv The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells.
EEA Mercedes
Fil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suvá, Mariana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Munilla, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina
Fil: Rodriguez-Alvarez, Lleretny. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Velasquez, Alejandra. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Briski, Olinda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-09T13:21:44Z
2020-06-09T13:21:44Z
2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7380
https://www.sciencedirect.com/science/article/abs/pii/S0093691X20301576
0093-691X
1879-3231
https://doi.org/10.1016/j.theriogenology.2020.02.045
url http://hdl.handle.net/20.500.12123/7380
https://www.sciencedirect.com/science/article/abs/pii/S0093691X20301576
https://doi.org/10.1016/j.theriogenology.2020.02.045
identifier_str_mv 0093-691X
1879-3231
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Theriogenology 148 : 140-148 (May 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.711113

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