Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation

Autores
González, Noelia; Gil, María Lydia; Rios, Glenda Laura; Canizo, Jésica Romina; De Blas, José Ignacio; Alberio, Ricardo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution. The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively. Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option.
EEA Balcarce
Fil: González, Noelia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España
Fil: Gil, Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España
Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Canizo, Jésica Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: De Blas, José Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Infectious Diseases Unit; España
Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fuente
The Open Veterinary Science Journal 8 : 1-5 (2014)
Materia
Óvulo
Perro
Experimentación in Vitro
Ova
Dogs
In Vitro Experimentation
Ovocitos
Atmósfera Gaseosa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4331
Acceder
id INTADig_ef7dbf49ba55669324a50874dc2a79eb
oai_identifier_str oai:localhost:20.500.12123/4331
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturationGonzález, NoeliaGil, María LydiaRios, Glenda LauraCanizo, Jésica RominaDe Blas, José IgnacioAlberio, RicardoÓvuloPerroExperimentación in VitroOvaDogsIn Vitro ExperimentationOvocitosAtmósfera GaseosaThe aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution. The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively. Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option.EEA BalcarceFil: González, Noelia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; EspañaFil: Gil, Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; EspañaFil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; ArgentinaFil: Canizo, Jésica Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; ArgentinaFil: De Blas, José Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Infectious Diseases Unit; EspañaFil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; ArgentinaBentham Open2019-01-25T12:21:33Z2019-01-25T12:21:33Z2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://benthamopen.com/contents/pdf/TOVSJ/TOVSJ-8-1.pdfhttp://hdl.handle.net/20.500.12123/43311874-3188https://doi.org/10.2174/1874318801408010001The Open Veterinary Science Journal 8 : 1-5 (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:33Zoai:localhost:20.500.12123/4331instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.135INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
title Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
spellingShingle Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
González, Noelia
Óvulo
Perro
Experimentación in Vitro
Ova
Dogs
In Vitro Experimentation
Ovocitos
Atmósfera Gaseosa
title_short Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
title_full Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
title_fullStr Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
title_full_unstemmed Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
title_sort Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
dc.creator.none.fl_str_mv González, Noelia
Gil, María Lydia
Rios, Glenda Laura
Canizo, Jésica Romina
De Blas, José Ignacio
Alberio, Ricardo
author González, Noelia
author_facet González, Noelia
Gil, María Lydia
Rios, Glenda Laura
Canizo, Jésica Romina
De Blas, José Ignacio
Alberio, Ricardo
author_role author
author2 Gil, María Lydia
Rios, Glenda Laura
Canizo, Jésica Romina
De Blas, José Ignacio
Alberio, Ricardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Óvulo
Perro
Experimentación in Vitro
Ova
Dogs
In Vitro Experimentation
Ovocitos
Atmósfera Gaseosa
topic Óvulo
Perro
Experimentación in Vitro
Ova
Dogs
In Vitro Experimentation
Ovocitos
Atmósfera Gaseosa
dc.description.none.fl_txt_mv The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution. The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively. Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option.
EEA Balcarce
Fil: González, Noelia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España
Fil: Gil, Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España
Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Canizo, Jésica Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: De Blas, José Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Infectious Diseases Unit; España
Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
description The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution. The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively. Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
2019-01-25T12:21:33Z
2019-01-25T12:21:33Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://benthamopen.com/contents/pdf/TOVSJ/TOVSJ-8-1.pdf
http://hdl.handle.net/20.500.12123/4331
1874-3188
https://doi.org/10.2174/1874318801408010001
url https://benthamopen.com/contents/pdf/TOVSJ/TOVSJ-8-1.pdf
http://hdl.handle.net/20.500.12123/4331
https://doi.org/10.2174/1874318801408010001
identifier_str_mv 1874-3188
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bentham Open
publisher.none.fl_str_mv Bentham Open
dc.source.none.fl_str_mv The Open Veterinary Science Journal 8 : 1-5 (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.891075

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