Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake
- Autores
- Sarli, Macarena; Rossner, Maria Victoria; Miró, María Victoria; Lanusse, Carlos Edmundo; Segovia Stefani, Lautaro Ezequiel; Cabaña, Norma Elía; Nava, Santiago; Lifschitz, Adrian Luis
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aims of this work were to evaluate the relevance of the tick attachment site on the host’s body in the fluazuron (FZN) uptake by ticks through direct contact, characterizing the early distribution of the drug through the skin and adipose tissue of different anatomical regions. Additionally, characterize how the systemic exposure (plasma availability) of FZN affects its efficacy against Rhipicephalus microplus. Five heifers were experimentally infested with R. microplus larvae and topically treated with FZN. Ticks exposed (TE) to direct topical contact with the drug—either in the dorsal (DTE) or ventral (VTE) areas of each heifer's body—or not exposed (TNE), were collected at 12 and 24 h post-treatment (PT). Skin and adipose tissue biopsies were taken at 12 h, 1 day and 10 days PT. Fluazuron concentrations in plasma, tissues and ticks were measured HPLC. Tick counts were performed from day 0 to day 38 PT. Mean FZN concentrations were similar in DTE and VTE and significantly higher than in TNE at 24 h PT. Dorsal and ventral skin samples showed comparable FZN levels. The area under the plasma concentration–time curves (AUC)₀₋₃₁ ranged from 589 to 1276 ng⋅d/mL. A negative correlation was observed between FZN plasma AUC3–17 and tick counts at day 17 PT. Results confirm that FZN is absorbed through the tick’s integument by direct contact independently of the attachment site. Fluazuron plasma concentrations of 20–25 ng/mL appear necessary to achieve optimal efficacy. Inter-animal variability in FZN exposure after topical application affects treatment efficacy against cattle ticks.
Asociación Cooperadora INTA Rafaela. Agencia Nacional de Promoción Científica y Tecnológica (PICT-2021–0278). Agencia Santafesina de Ciencia, Tecnología e Innovación (PEICID-2022–049). Secretaría de Innovación, Ciencia y Tecnología (Proyectos de Redes Federales de Alto Impacto: REDGARR 100390265)
EEA Rafaela
Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina
Fil: Miró, Maria Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil. Laboratorio de Farmacología; Argentina.
Fil: Miró, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fil: Lanusse, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.
Fil: Lanusse, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN). Tandil; Argentina
Fil: Segovia Stefani, Lautaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; Argentina
Fil: Cabaña, Norma Elía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.
Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina - Fuente
- Veterinary Parasitology 338 : 110531 (August 2025)
- Materia
-
Rhipicephalus
Sistema de Administración de Drogas
Biodisponibilidad
Aplicación Local
Ganado Bovino
Garrapata
Drug Delivery Systems
Bioavailability
Topical Application
Cattle
Ticks
Rhipicephalus microplus
Fluazuron - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/22858
Ver los metadatos del registro completo
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Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptakeSarli, MacarenaRossner, Maria VictoriaMiró, María VictoriaLanusse, Carlos EdmundoSegovia Stefani, Lautaro EzequielCabaña, Norma ElíaNava, SantiagoLifschitz, Adrian LuisRhipicephalusSistema de Administración de DrogasBiodisponibilidadAplicación LocalGanado BovinoGarrapataDrug Delivery SystemsBioavailabilityTopical ApplicationCattleTicksRhipicephalus microplusFluazuronThe aims of this work were to evaluate the relevance of the tick attachment site on the host’s body in the fluazuron (FZN) uptake by ticks through direct contact, characterizing the early distribution of the drug through the skin and adipose tissue of different anatomical regions. Additionally, characterize how the systemic exposure (plasma availability) of FZN affects its efficacy against Rhipicephalus microplus. Five heifers were experimentally infested with R. microplus larvae and topically treated with FZN. Ticks exposed (TE) to direct topical contact with the drug—either in the dorsal (DTE) or ventral (VTE) areas of each heifer's body—or not exposed (TNE), were collected at 12 and 24 h post-treatment (PT). Skin and adipose tissue biopsies were taken at 12 h, 1 day and 10 days PT. Fluazuron concentrations in plasma, tissues and ticks were measured HPLC. Tick counts were performed from day 0 to day 38 PT. Mean FZN concentrations were similar in DTE and VTE and significantly higher than in TNE at 24 h PT. Dorsal and ventral skin samples showed comparable FZN levels. The area under the plasma concentration–time curves (AUC)₀₋₃₁ ranged from 589 to 1276 ng⋅d/mL. A negative correlation was observed between FZN plasma AUC3–17 and tick counts at day 17 PT. Results confirm that FZN is absorbed through the tick’s integument by direct contact independently of the attachment site. Fluazuron plasma concentrations of 20–25 ng/mL appear necessary to achieve optimal efficacy. Inter-animal variability in FZN exposure after topical application affects treatment efficacy against cattle ticks.Asociación Cooperadora INTA Rafaela. Agencia Nacional de Promoción Científica y Tecnológica (PICT-2021–0278). Agencia Santafesina de Ciencia, Tecnología e Innovación (PEICID-2022–049). Secretaría de Innovación, Ciencia y Tecnología (Proyectos de Redes Federales de Alto Impacto: REDGARR 100390265)EEA RafaelaFil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; ArgentinaFil: Miró, Maria Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil. Laboratorio de Farmacología; Argentina.Fil: Miró, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); ArgentinaFil: Lanusse, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.Fil: Lanusse, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN). Tandil; ArgentinaFil: Segovia Stefani, Lautaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; ArgentinaFil: Cabaña, Norma Elía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); ArgentinaElsevier2025-07-02T12:21:52Z2025-07-02T12:21:52Z2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22858https://www.sciencedirect.com/science/article/abs/pii/S03044017250014260304-4017https://doi.org/10.1016/j.vetpar.2025.110531Veterinary Parasitology 338 : 110531 (August 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L01-I044, Enfermedades subtropicales con impacto sobre la producción, salud pública y sustentabilidad en la ganadería del norte de Argentinainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:22Zoai:localhost:20.500.12123/22858instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:22.799INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake |
| title |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake |
| spellingShingle |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake Sarli, Macarena Rhipicephalus Sistema de Administración de Drogas Biodisponibilidad Aplicación Local Ganado Bovino Garrapata Drug Delivery Systems Bioavailability Topical Application Cattle Ticks Rhipicephalus microplus Fluazuron |
| title_short |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake |
| title_full |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake |
| title_fullStr |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake |
| title_full_unstemmed |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake |
| title_sort |
Fluazuron efficacy against Rhipicephalus microplus (Acari: Ixodidae) in cattle: Systemic exposure and pattern of tick drug uptake |
| dc.creator.none.fl_str_mv |
Sarli, Macarena Rossner, Maria Victoria Miró, María Victoria Lanusse, Carlos Edmundo Segovia Stefani, Lautaro Ezequiel Cabaña, Norma Elía Nava, Santiago Lifschitz, Adrian Luis |
| author |
Sarli, Macarena |
| author_facet |
Sarli, Macarena Rossner, Maria Victoria Miró, María Victoria Lanusse, Carlos Edmundo Segovia Stefani, Lautaro Ezequiel Cabaña, Norma Elía Nava, Santiago Lifschitz, Adrian Luis |
| author_role |
author |
| author2 |
Rossner, Maria Victoria Miró, María Victoria Lanusse, Carlos Edmundo Segovia Stefani, Lautaro Ezequiel Cabaña, Norma Elía Nava, Santiago Lifschitz, Adrian Luis |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Rhipicephalus Sistema de Administración de Drogas Biodisponibilidad Aplicación Local Ganado Bovino Garrapata Drug Delivery Systems Bioavailability Topical Application Cattle Ticks Rhipicephalus microplus Fluazuron |
| topic |
Rhipicephalus Sistema de Administración de Drogas Biodisponibilidad Aplicación Local Ganado Bovino Garrapata Drug Delivery Systems Bioavailability Topical Application Cattle Ticks Rhipicephalus microplus Fluazuron |
| dc.description.none.fl_txt_mv |
The aims of this work were to evaluate the relevance of the tick attachment site on the host’s body in the fluazuron (FZN) uptake by ticks through direct contact, characterizing the early distribution of the drug through the skin and adipose tissue of different anatomical regions. Additionally, characterize how the systemic exposure (plasma availability) of FZN affects its efficacy against Rhipicephalus microplus. Five heifers were experimentally infested with R. microplus larvae and topically treated with FZN. Ticks exposed (TE) to direct topical contact with the drug—either in the dorsal (DTE) or ventral (VTE) areas of each heifer's body—or not exposed (TNE), were collected at 12 and 24 h post-treatment (PT). Skin and adipose tissue biopsies were taken at 12 h, 1 day and 10 days PT. Fluazuron concentrations in plasma, tissues and ticks were measured HPLC. Tick counts were performed from day 0 to day 38 PT. Mean FZN concentrations were similar in DTE and VTE and significantly higher than in TNE at 24 h PT. Dorsal and ventral skin samples showed comparable FZN levels. The area under the plasma concentration–time curves (AUC)₀₋₃₁ ranged from 589 to 1276 ng⋅d/mL. A negative correlation was observed between FZN plasma AUC3–17 and tick counts at day 17 PT. Results confirm that FZN is absorbed through the tick’s integument by direct contact independently of the attachment site. Fluazuron plasma concentrations of 20–25 ng/mL appear necessary to achieve optimal efficacy. Inter-animal variability in FZN exposure after topical application affects treatment efficacy against cattle ticks. Asociación Cooperadora INTA Rafaela. Agencia Nacional de Promoción Científica y Tecnológica (PICT-2021–0278). Agencia Santafesina de Ciencia, Tecnología e Innovación (PEICID-2022–049). Secretaría de Innovación, Ciencia y Tecnología (Proyectos de Redes Federales de Alto Impacto: REDGARR 100390265) EEA Rafaela Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina Fil: Miró, Maria Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil. Laboratorio de Farmacología; Argentina. Fil: Miró, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Lanusse, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina. Fil: Lanusse, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN). Tandil; Argentina Fil: Segovia Stefani, Lautaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; Argentina Fil: Cabaña, Norma Elía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina. Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina |
| description |
The aims of this work were to evaluate the relevance of the tick attachment site on the host’s body in the fluazuron (FZN) uptake by ticks through direct contact, characterizing the early distribution of the drug through the skin and adipose tissue of different anatomical regions. Additionally, characterize how the systemic exposure (plasma availability) of FZN affects its efficacy against Rhipicephalus microplus. Five heifers were experimentally infested with R. microplus larvae and topically treated with FZN. Ticks exposed (TE) to direct topical contact with the drug—either in the dorsal (DTE) or ventral (VTE) areas of each heifer's body—or not exposed (TNE), were collected at 12 and 24 h post-treatment (PT). Skin and adipose tissue biopsies were taken at 12 h, 1 day and 10 days PT. Fluazuron concentrations in plasma, tissues and ticks were measured HPLC. Tick counts were performed from day 0 to day 38 PT. Mean FZN concentrations were similar in DTE and VTE and significantly higher than in TNE at 24 h PT. Dorsal and ventral skin samples showed comparable FZN levels. The area under the plasma concentration–time curves (AUC)₀₋₃₁ ranged from 589 to 1276 ng⋅d/mL. A negative correlation was observed between FZN plasma AUC3–17 and tick counts at day 17 PT. Results confirm that FZN is absorbed through the tick’s integument by direct contact independently of the attachment site. Fluazuron plasma concentrations of 20–25 ng/mL appear necessary to achieve optimal efficacy. Inter-animal variability in FZN exposure after topical application affects treatment efficacy against cattle ticks. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025-07-02T12:21:52Z 2025-07-02T12:21:52Z 2025-06 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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| url |
http://hdl.handle.net/20.500.12123/22858 https://www.sciencedirect.com/science/article/abs/pii/S0304401725001426 https://doi.org/10.1016/j.vetpar.2025.110531 |
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0304-4017 |
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eng |
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eng |
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info:eu-repograntAgreement/INTA/2023-PE-L01-I044, Enfermedades subtropicales con impacto sobre la producción, salud pública y sustentabilidad en la ganadería del norte de Argentina |
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info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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application/pdf |
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Elsevier |
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