Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake

Autores
Sarli, Macarena; Miró, María Victoria; Rossner, Maria Victoria; Gomez, Viviana Daniela; Torrents, Jorgelina; Nava, Santiago; Lifschitz, Adrián Luis
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Relationship between fluazuron (FZN) concentrations in cattle plasma and ticks and its therapeutic efficacy percentage (EP) against Rhipicephalus microplus was analyzed. The extent of FZN uptake by ticks after its topical administration was also evaluated. Heifers, naturally infested with R. microplus, were divided in treated and no treated groups. Blood and tick samples were taken to measure FZN concentrations and female ticks were counted to evaluate the EP of the treatment from day 0 to 45 post-treatment (PT). Ticks exposed (TE) and not exposed (TNE) to topical contact with the drug were collected at 12 and 24 h PT. Artificial feeding (AF) of ticks was also performed with blood from a treated heifer. The EP on days 21, 28, 35, 42 and 45 PT was 99.1, 92.3, 81.2, 74.7 and 76.5 %, respectively. Fluazuron disposition within ticks during the first 2 days post-treatment did not mirror the corresponding FZN levels in the bovine plasma, but a statistically significant positive correlation was evident from day 3. Mean FZN concentrations in TE at 12 h and 24 h PT were significantly higher than in TNE. The FZN concentrations in AF ticks were significantly lower than those in TE but comparable to the levels in TNE. The efficacy and pharmacokinetics analysis suggest that maintaining a threshold FZN plasma concentration above 20–25 ng/mL is crucial to prevent the development of larvae into engorged females. The results also indicate that direct absorption of FZN through the tick's integument occurs in the initial days after treatment.
Asociacion Cooperadora INTA Rafaela. Agencia Nacional de Promocion Científica y Tecnologica (PICT-2021-0278). Agencia Santafesina de Ciencia, Tecnología e Innovacion (PEICID2022-049). Secretaría de Innovacion, Ciencia y Tecnología (Proyectos de Redes Federales de Alto Impacto: REDGARR 100390265)
EEA Rafaela
Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Miró, V. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.
Fil: Miró, V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina
Fil: Gomez, Viviana Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina
Fil: Torrents, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Torrents, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.
Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fuente
Research in Veterinary Science 180 : 105402 (November 2024)
Materia
Cattle
Acaricides
Drugs
Pharmacokinetics
Pharmacodynamics
Ganado Bovino
Acaricidas
Medicamentos
Farmacocinética
Farmacodinámica
Rhipicephalus microplus
Fluazurón
Control de Garrapatas
Fluazuron
Tick Control
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/19511
Acceder
id INTADig_a88c1e283ede6d2b8eefd266d1f433b1
oai_identifier_str oai:localhost:20.500.12123/19511
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptakeSarli, MacarenaMiró, María VictoriaRossner, Maria VictoriaGomez, Viviana DanielaTorrents, JorgelinaNava, SantiagoLifschitz, Adrián LuisCattleAcaricidesDrugsPharmacokineticsPharmacodynamicsGanado BovinoAcaricidasMedicamentosFarmacocinéticaFarmacodinámicaRhipicephalus microplusFluazurónControl de GarrapatasFluazuronTick ControlRelationship between fluazuron (FZN) concentrations in cattle plasma and ticks and its therapeutic efficacy percentage (EP) against Rhipicephalus microplus was analyzed. The extent of FZN uptake by ticks after its topical administration was also evaluated. Heifers, naturally infested with R. microplus, were divided in treated and no treated groups. Blood and tick samples were taken to measure FZN concentrations and female ticks were counted to evaluate the EP of the treatment from day 0 to 45 post-treatment (PT). Ticks exposed (TE) and not exposed (TNE) to topical contact with the drug were collected at 12 and 24 h PT. Artificial feeding (AF) of ticks was also performed with blood from a treated heifer. The EP on days 21, 28, 35, 42 and 45 PT was 99.1, 92.3, 81.2, 74.7 and 76.5 %, respectively. Fluazuron disposition within ticks during the first 2 days post-treatment did not mirror the corresponding FZN levels in the bovine plasma, but a statistically significant positive correlation was evident from day 3. Mean FZN concentrations in TE at 12 h and 24 h PT were significantly higher than in TNE. The FZN concentrations in AF ticks were significantly lower than those in TE but comparable to the levels in TNE. The efficacy and pharmacokinetics analysis suggest that maintaining a threshold FZN plasma concentration above 20–25 ng/mL is crucial to prevent the development of larvae into engorged females. The results also indicate that direct absorption of FZN through the tick's integument occurs in the initial days after treatment.Asociacion Cooperadora INTA Rafaela. Agencia Nacional de Promocion Científica y Tecnologica (PICT-2021-0278). Agencia Santafesina de Ciencia, Tecnología e Innovacion (PEICID2022-049). Secretaría de Innovacion, Ciencia y Tecnología (Proyectos de Redes Federales de Alto Impacto: REDGARR 100390265)EEA RafaelaFil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Miró, V. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.Fil: Miró, V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); ArgentinaFil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; ArgentinaFil: Gomez, Viviana Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; ArgentinaFil: Torrents, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Torrents, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); ArgentinaElsevier2024-09-24T10:56:03Z2024-09-24T10:56:03Z2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/19511https://www.sciencedirect.com/science/article/pii/S00345288240026980034-5288https://doi.org/10.1016/j.rvsc.2024.105402Research in Veterinary Science 180 : 105402 (November 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y lainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:54Zoai:localhost:20.500.12123/19511instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:54.882INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
title Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
spellingShingle Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
Sarli, Macarena
Cattle
Acaricides
Drugs
Pharmacokinetics
Pharmacodynamics
Ganado Bovino
Acaricidas
Medicamentos
Farmacocinética
Farmacodinámica
Rhipicephalus microplus
Fluazurón
Control de Garrapatas
Fluazuron
Tick Control
title_short Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
title_full Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
title_fullStr Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
title_full_unstemmed Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
title_sort Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
dc.creator.none.fl_str_mv Sarli, Macarena
Miró, María Victoria
Rossner, Maria Victoria
Gomez, Viviana Daniela
Torrents, Jorgelina
Nava, Santiago
Lifschitz, Adrián Luis
author Sarli, Macarena
author_facet Sarli, Macarena
Miró, María Victoria
Rossner, Maria Victoria
Gomez, Viviana Daniela
Torrents, Jorgelina
Nava, Santiago
Lifschitz, Adrián Luis
author_role author
author2 Miró, María Victoria
Rossner, Maria Victoria
Gomez, Viviana Daniela
Torrents, Jorgelina
Nava, Santiago
Lifschitz, Adrián Luis
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cattle
Acaricides
Drugs
Pharmacokinetics
Pharmacodynamics
Ganado Bovino
Acaricidas
Medicamentos
Farmacocinética
Farmacodinámica
Rhipicephalus microplus
Fluazurón
Control de Garrapatas
Fluazuron
Tick Control
topic Cattle
Acaricides
Drugs
Pharmacokinetics
Pharmacodynamics
Ganado Bovino
Acaricidas
Medicamentos
Farmacocinética
Farmacodinámica
Rhipicephalus microplus
Fluazurón
Control de Garrapatas
Fluazuron
Tick Control
dc.description.none.fl_txt_mv Relationship between fluazuron (FZN) concentrations in cattle plasma and ticks and its therapeutic efficacy percentage (EP) against Rhipicephalus microplus was analyzed. The extent of FZN uptake by ticks after its topical administration was also evaluated. Heifers, naturally infested with R. microplus, were divided in treated and no treated groups. Blood and tick samples were taken to measure FZN concentrations and female ticks were counted to evaluate the EP of the treatment from day 0 to 45 post-treatment (PT). Ticks exposed (TE) and not exposed (TNE) to topical contact with the drug were collected at 12 and 24 h PT. Artificial feeding (AF) of ticks was also performed with blood from a treated heifer. The EP on days 21, 28, 35, 42 and 45 PT was 99.1, 92.3, 81.2, 74.7 and 76.5 %, respectively. Fluazuron disposition within ticks during the first 2 days post-treatment did not mirror the corresponding FZN levels in the bovine plasma, but a statistically significant positive correlation was evident from day 3. Mean FZN concentrations in TE at 12 h and 24 h PT were significantly higher than in TNE. The FZN concentrations in AF ticks were significantly lower than those in TE but comparable to the levels in TNE. The efficacy and pharmacokinetics analysis suggest that maintaining a threshold FZN plasma concentration above 20–25 ng/mL is crucial to prevent the development of larvae into engorged females. The results also indicate that direct absorption of FZN through the tick's integument occurs in the initial days after treatment.
Asociacion Cooperadora INTA Rafaela. Agencia Nacional de Promocion Científica y Tecnologica (PICT-2021-0278). Agencia Santafesina de Ciencia, Tecnología e Innovacion (PEICID2022-049). Secretaría de Innovacion, Ciencia y Tecnología (Proyectos de Redes Federales de Alto Impacto: REDGARR 100390265)
EEA Rafaela
Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Miró, V. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.
Fil: Miró, V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina
Fil: Gomez, Viviana Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina
Fil: Torrents, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Torrents, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina.
Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
description Relationship between fluazuron (FZN) concentrations in cattle plasma and ticks and its therapeutic efficacy percentage (EP) against Rhipicephalus microplus was analyzed. The extent of FZN uptake by ticks after its topical administration was also evaluated. Heifers, naturally infested with R. microplus, were divided in treated and no treated groups. Blood and tick samples were taken to measure FZN concentrations and female ticks were counted to evaluate the EP of the treatment from day 0 to 45 post-treatment (PT). Ticks exposed (TE) and not exposed (TNE) to topical contact with the drug were collected at 12 and 24 h PT. Artificial feeding (AF) of ticks was also performed with blood from a treated heifer. The EP on days 21, 28, 35, 42 and 45 PT was 99.1, 92.3, 81.2, 74.7 and 76.5 %, respectively. Fluazuron disposition within ticks during the first 2 days post-treatment did not mirror the corresponding FZN levels in the bovine plasma, but a statistically significant positive correlation was evident from day 3. Mean FZN concentrations in TE at 12 h and 24 h PT were significantly higher than in TNE. The FZN concentrations in AF ticks were significantly lower than those in TE but comparable to the levels in TNE. The efficacy and pharmacokinetics analysis suggest that maintaining a threshold FZN plasma concentration above 20–25 ng/mL is crucial to prevent the development of larvae into engorged females. The results also indicate that direct absorption of FZN through the tick's integument occurs in the initial days after treatment.
publishDate 2024
dc.date.none.fl_str_mv 2024-09-24T10:56:03Z
2024-09-24T10:56:03Z
2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/19511
https://www.sciencedirect.com/science/article/pii/S0034528824002698
0034-5288
https://doi.org/10.1016/j.rvsc.2024.105402
url http://hdl.handle.net/20.500.12123/19511
https://www.sciencedirect.com/science/article/pii/S0034528824002698
https://doi.org/10.1016/j.rvsc.2024.105402
identifier_str_mv 0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in Veterinary Science 180 : 105402 (November 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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