Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina

Autores
Bessone, Fernando Aníbal; Bessone, Gabriela; Marini, Sebastián José; Conde, María Belén; Alustiza, Fabrisio Eduardo; Zielinski, Gustavo Carlos
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. Aim: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina. Materials and Methods: Intensive pig farms from the central region of Argentina were sampled. Intestinal mucosa swabs from pigs with diarrhea were taken, seeded on MacConkey agar plates, biochemically typified and tested by polymerase chain reaction (PCR). Antibiograms were made by disk-diffusion method. Results: A total of 54 strains from clinical cases studied showed PCR findings: 88.88% (48/54) expressed at least one gene coding for a virulence factor. Colonization factors found were: 39.58% of strains had F18, 33.33% were F4 and 31.25% adhesin involved in diffuse adherence-I; 29.17%, 25%, and 2.1% expressed LT, STb, and STa, respectively. 25% were STx and 16.67% were eae positive. Only 2.1% were STx2. The most active antibiotics against most strains were gentamicin and ceftiofur, but resistance profiles against many antibiotics were found. Conclusion: High circulation of pathogens strains of E. coli among pigs with diarrhea with an extended antibiotic resistance profile.
EEA Marcos Juárez
Fil: Bessone, Fernando Aní­bal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Bessone, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Marini, Sebastián José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fuente
Veterinary World 10 (8) : 939-945 (August 2017)
Materia
Cerdo
Enfermedades de los Animales
Escherichia coli
Virulencia
Resistencia a los Antibióticos
Genética
Swine
Animal Diseases
Virulence
Resistance to Antibiotics
Genetics
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3656
Acceder
id INTADig_fa25b360d313d57d451ba3d8742eba4b
oai_identifier_str oai:localhost:20.500.12123/3656
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of ArgentinaBessone, Fernando AníbalBessone, GabrielaMarini, Sebastián JoséConde, María BelénAlustiza, Fabrisio EduardoZielinski, Gustavo CarlosCerdoEnfermedades de los AnimalesEscherichia coliVirulenciaResistencia a los AntibióticosGenéticaSwineAnimal DiseasesVirulenceResistance to AntibioticsGeneticsArgentinaBackground: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. Aim: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina. Materials and Methods: Intensive pig farms from the central region of Argentina were sampled. Intestinal mucosa swabs from pigs with diarrhea were taken, seeded on MacConkey agar plates, biochemically typified and tested by polymerase chain reaction (PCR). Antibiograms were made by disk-diffusion method. Results: A total of 54 strains from clinical cases studied showed PCR findings: 88.88% (48/54) expressed at least one gene coding for a virulence factor. Colonization factors found were: 39.58% of strains had F18, 33.33% were F4 and 31.25% adhesin involved in diffuse adherence-I; 29.17%, 25%, and 2.1% expressed LT, STb, and STa, respectively. 25% were STx and 16.67% were eae positive. Only 2.1% were STx2. The most active antibiotics against most strains were gentamicin and ceftiofur, but resistance profiles against many antibiotics were found. Conclusion: High circulation of pathogens strains of E. coli among pigs with diarrhea with an extended antibiotic resistance profile.EEA Marcos JuárezFil: Bessone, Fernando Aní­bal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Bessone, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Marini, Sebastián José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina2018-10-22T11:49:09Z2018-10-22T11:49:09Z2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3656http://www.veterinaryworld.org/Vol.10/August-2017/18.html0972-89882231-0916https://doi.org/10.14202/vetworld.2017.939-945Veterinary World 10 (8) : 939-945 (August 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:42Zoai:localhost:20.500.12123/3656instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:42.576INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
title Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
spellingShingle Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
Bessone, Fernando Aníbal
Cerdo
Enfermedades de los Animales
Escherichia coli
Virulencia
Resistencia a los Antibióticos
Genética
Swine
Animal Diseases
Virulence
Resistance to Antibiotics
Genetics
Argentina
title_short Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
title_full Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
title_fullStr Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
title_full_unstemmed Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
title_sort Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
dc.creator.none.fl_str_mv Bessone, Fernando Aníbal
Bessone, Gabriela
Marini, Sebastián José
Conde, María Belén
Alustiza, Fabrisio Eduardo
Zielinski, Gustavo Carlos
author Bessone, Fernando Aníbal
author_facet Bessone, Fernando Aníbal
Bessone, Gabriela
Marini, Sebastián José
Conde, María Belén
Alustiza, Fabrisio Eduardo
Zielinski, Gustavo Carlos
author_role author
author2 Bessone, Gabriela
Marini, Sebastián José
Conde, María Belén
Alustiza, Fabrisio Eduardo
Zielinski, Gustavo Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cerdo
Enfermedades de los Animales
Escherichia coli
Virulencia
Resistencia a los Antibióticos
Genética
Swine
Animal Diseases
Virulence
Resistance to Antibiotics
Genetics
Argentina
topic Cerdo
Enfermedades de los Animales
Escherichia coli
Virulencia
Resistencia a los Antibióticos
Genética
Swine
Animal Diseases
Virulence
Resistance to Antibiotics
Genetics
Argentina
dc.description.none.fl_txt_mv Background: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. Aim: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina. Materials and Methods: Intensive pig farms from the central region of Argentina were sampled. Intestinal mucosa swabs from pigs with diarrhea were taken, seeded on MacConkey agar plates, biochemically typified and tested by polymerase chain reaction (PCR). Antibiograms were made by disk-diffusion method. Results: A total of 54 strains from clinical cases studied showed PCR findings: 88.88% (48/54) expressed at least one gene coding for a virulence factor. Colonization factors found were: 39.58% of strains had F18, 33.33% were F4 and 31.25% adhesin involved in diffuse adherence-I; 29.17%, 25%, and 2.1% expressed LT, STb, and STa, respectively. 25% were STx and 16.67% were eae positive. Only 2.1% were STx2. The most active antibiotics against most strains were gentamicin and ceftiofur, but resistance profiles against many antibiotics were found. Conclusion: High circulation of pathogens strains of E. coli among pigs with diarrhea with an extended antibiotic resistance profile.
EEA Marcos Juárez
Fil: Bessone, Fernando Aní­bal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Bessone, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Marini, Sebastián José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
description Background: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. Aim: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina. Materials and Methods: Intensive pig farms from the central region of Argentina were sampled. Intestinal mucosa swabs from pigs with diarrhea were taken, seeded on MacConkey agar plates, biochemically typified and tested by polymerase chain reaction (PCR). Antibiograms were made by disk-diffusion method. Results: A total of 54 strains from clinical cases studied showed PCR findings: 88.88% (48/54) expressed at least one gene coding for a virulence factor. Colonization factors found were: 39.58% of strains had F18, 33.33% were F4 and 31.25% adhesin involved in diffuse adherence-I; 29.17%, 25%, and 2.1% expressed LT, STb, and STa, respectively. 25% were STx and 16.67% were eae positive. Only 2.1% were STx2. The most active antibiotics against most strains were gentamicin and ceftiofur, but resistance profiles against many antibiotics were found. Conclusion: High circulation of pathogens strains of E. coli among pigs with diarrhea with an extended antibiotic resistance profile.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
2018-10-22T11:49:09Z
2018-10-22T11:49:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3656
http://www.veterinaryworld.org/Vol.10/August-2017/18.html
0972-8988
2231-0916
https://doi.org/10.14202/vetworld.2017.939-945
url http://hdl.handle.net/20.500.12123/3656
http://www.veterinaryworld.org/Vol.10/August-2017/18.html
https://doi.org/10.14202/vetworld.2017.939-945
identifier_str_mv 0972-8988
2231-0916
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Veterinary World 10 (8) : 939-945 (August 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846787513575800832
score 12.982451

Publicaciones similares