Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina

Autores
Aban, Carla Luciana; Taboada, Gisel María; Spedaleti, Yamila Andrea; Maita, Efraín; Galvan, Marta Zulema
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
S. sclerotiorum is the causal agent of the white mould disease on common bean crops, one of the most threatening fungal diseases occurring across major bean production regions. Currently, there are no commercial cultivars with high levels of genetic resistance to white mould. The aim of this study was to analyse the population structure of 109 isolates of S. sclerotiorum from six dry bean fields in the main production area of Argentina using nine microsatellite loci. A total of 30 multilocus haplotypes (MLHs) were identified, of which 18 MLHs were unique. The remaining 12 MLHs were constituted by 83% of the isolates, six MLHs of which (composed of 75 isolates) were shared at least between two locations. Population genetic structure analysis and discriminant analysis of principal components (DAPC) identified two genetic clusters (subpopulations). The genetic cluster 1 (GC 1) was composed of 21 isolates and 15 MLHs. Similarly, the genetic cluster 2 (GC 2) was composed of 23 isolates and 15 MLHs. These two genetic clusters were observed in most locations sampled. Low levels of genetic differentiation (ΦST = 0.198; P < 0.0001) followed by high levels of gene flow (Nm > 1) between genetic clusters were observed. Linkage disequilibrium analysis showed that one of the two genetic subpopulations was under linkage equilibrium (P > 0.001), which is consistent with recombinant populations. These results suggest the occurrence of both modes of reproductive behaviour, clonal and recombining, compromising the durability of management strategies for white mould disease in common bean cultivars.
EEA Salta
Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Spedaleti, Yamila Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Maita, Efraín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Maita, Efraín Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Investigaciones Botánicas; Argentina
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fuente
European Journal of Plant Pathology (Published: 10 May 2021)
Materia
Fríjol (phaseolus)
Sclerotinia sclerotiorum
Hongos
Estructura de la Población
Argentina
Kidney Beans
Fungi
Population Structure
Common Beans
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9422
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9422
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of ArgentinaAban, Carla LucianaTaboada, Gisel MaríaSpedaleti, Yamila AndreaMaita, EfraínGalvan, Marta ZulemaFríjol (phaseolus)Sclerotinia sclerotiorumHongosEstructura de la PoblaciónArgentinaKidney BeansFungiPopulation StructureCommon BeansS. sclerotiorum is the causal agent of the white mould disease on common bean crops, one of the most threatening fungal diseases occurring across major bean production regions. Currently, there are no commercial cultivars with high levels of genetic resistance to white mould. The aim of this study was to analyse the population structure of 109 isolates of S. sclerotiorum from six dry bean fields in the main production area of Argentina using nine microsatellite loci. A total of 30 multilocus haplotypes (MLHs) were identified, of which 18 MLHs were unique. The remaining 12 MLHs were constituted by 83% of the isolates, six MLHs of which (composed of 75 isolates) were shared at least between two locations. Population genetic structure analysis and discriminant analysis of principal components (DAPC) identified two genetic clusters (subpopulations). The genetic cluster 1 (GC 1) was composed of 21 isolates and 15 MLHs. Similarly, the genetic cluster 2 (GC 2) was composed of 23 isolates and 15 MLHs. These two genetic clusters were observed in most locations sampled. Low levels of genetic differentiation (ΦST = 0.198; P < 0.0001) followed by high levels of gene flow (Nm > 1) between genetic clusters were observed. Linkage disequilibrium analysis showed that one of the two genetic subpopulations was under linkage equilibrium (P > 0.001), which is consistent with recombinant populations. These results suggest the occurrence of both modes of reproductive behaviour, clonal and recombining, compromising the durability of management strategies for white mould disease in common bean cultivars.EEA SaltaFil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Spedaleti, Yamila Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Maita, Efraín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Maita, Efraín Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Investigaciones Botánicas; ArgentinaFil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaSpringer2021-05-26T12:32:30Z2021-05-26T12:32:30Z2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9422https://link.springer.com/article/10.1007/s10658-021-02288-70929-18731573-8469https://doi.org/10.1007/s10658-021-02288-7European Journal of Plant Pathology (Published: 10 May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:55Zoai:localhost:20.500.12123/9422instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:55.586INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
title Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
spellingShingle Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
Aban, Carla Luciana
Fríjol (phaseolus)
Sclerotinia sclerotiorum
Hongos
Estructura de la Población
Argentina
Kidney Beans
Fungi
Population Structure
Common Beans
title_short Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
title_full Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
title_fullStr Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
title_full_unstemmed Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
title_sort Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
dc.creator.none.fl_str_mv Aban, Carla Luciana
Taboada, Gisel María
Spedaleti, Yamila Andrea
Maita, Efraín
Galvan, Marta Zulema
author Aban, Carla Luciana
author_facet Aban, Carla Luciana
Taboada, Gisel María
Spedaleti, Yamila Andrea
Maita, Efraín
Galvan, Marta Zulema
author_role author
author2 Taboada, Gisel María
Spedaleti, Yamila Andrea
Maita, Efraín
Galvan, Marta Zulema
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fríjol (phaseolus)
Sclerotinia sclerotiorum
Hongos
Estructura de la Población
Argentina
Kidney Beans
Fungi
Population Structure
Common Beans
topic Fríjol (phaseolus)
Sclerotinia sclerotiorum
Hongos
Estructura de la Población
Argentina
Kidney Beans
Fungi
Population Structure
Common Beans
dc.description.none.fl_txt_mv S. sclerotiorum is the causal agent of the white mould disease on common bean crops, one of the most threatening fungal diseases occurring across major bean production regions. Currently, there are no commercial cultivars with high levels of genetic resistance to white mould. The aim of this study was to analyse the population structure of 109 isolates of S. sclerotiorum from six dry bean fields in the main production area of Argentina using nine microsatellite loci. A total of 30 multilocus haplotypes (MLHs) were identified, of which 18 MLHs were unique. The remaining 12 MLHs were constituted by 83% of the isolates, six MLHs of which (composed of 75 isolates) were shared at least between two locations. Population genetic structure analysis and discriminant analysis of principal components (DAPC) identified two genetic clusters (subpopulations). The genetic cluster 1 (GC 1) was composed of 21 isolates and 15 MLHs. Similarly, the genetic cluster 2 (GC 2) was composed of 23 isolates and 15 MLHs. These two genetic clusters were observed in most locations sampled. Low levels of genetic differentiation (ΦST = 0.198; P < 0.0001) followed by high levels of gene flow (Nm > 1) between genetic clusters were observed. Linkage disequilibrium analysis showed that one of the two genetic subpopulations was under linkage equilibrium (P > 0.001), which is consistent with recombinant populations. These results suggest the occurrence of both modes of reproductive behaviour, clonal and recombining, compromising the durability of management strategies for white mould disease in common bean cultivars.
EEA Salta
Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Spedaleti, Yamila Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Maita, Efraín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Maita, Efraín Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Investigaciones Botánicas; Argentina
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
description S. sclerotiorum is the causal agent of the white mould disease on common bean crops, one of the most threatening fungal diseases occurring across major bean production regions. Currently, there are no commercial cultivars with high levels of genetic resistance to white mould. The aim of this study was to analyse the population structure of 109 isolates of S. sclerotiorum from six dry bean fields in the main production area of Argentina using nine microsatellite loci. A total of 30 multilocus haplotypes (MLHs) were identified, of which 18 MLHs were unique. The remaining 12 MLHs were constituted by 83% of the isolates, six MLHs of which (composed of 75 isolates) were shared at least between two locations. Population genetic structure analysis and discriminant analysis of principal components (DAPC) identified two genetic clusters (subpopulations). The genetic cluster 1 (GC 1) was composed of 21 isolates and 15 MLHs. Similarly, the genetic cluster 2 (GC 2) was composed of 23 isolates and 15 MLHs. These two genetic clusters were observed in most locations sampled. Low levels of genetic differentiation (ΦST = 0.198; P < 0.0001) followed by high levels of gene flow (Nm > 1) between genetic clusters were observed. Linkage disequilibrium analysis showed that one of the two genetic subpopulations was under linkage equilibrium (P > 0.001), which is consistent with recombinant populations. These results suggest the occurrence of both modes of reproductive behaviour, clonal and recombining, compromising the durability of management strategies for white mould disease in common bean cultivars.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-26T12:32:30Z
2021-05-26T12:32:30Z
2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9422
https://link.springer.com/article/10.1007/s10658-021-02288-7
0929-1873
1573-8469
https://doi.org/10.1007/s10658-021-02288-7
url http://hdl.handle.net/20.500.12123/9422
https://link.springer.com/article/10.1007/s10658-021-02288-7
https://doi.org/10.1007/s10658-021-02288-7
identifier_str_mv 0929-1873
1573-8469
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European Journal of Plant Pathology (Published: 10 May 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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