Detection of swainsonine-producing endophytes in Patagonian Astragalus species

Autores
Martinez, Agustin; Robles, Carlos Alejandro; Roper, Jessie M.; Gardner, Dale R.; Neyaz, Marwah S.; Joelson, Natalia Zoe; Cook, Daniel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Swainsonine has been identified as the toxin in legumes belonging to the genera Astragalus and Oxytropis throughout the world including China, North America, and South America. Several South American Astragalus species have been reported to contain swainsonine; however, data is lacking to support the presence of a fungal symbiont in South American Astragalus species as has been shown for North American and Chinese Astragalus and Oxytropis species. The objective of this study was to investigate several South American species that have been reported to contain swainsonine for the presence of the fungal symbiont using culturing and PCR. Swainsonine was detected in field collections of A. pehuenches, A. illinii and A. chamissonis but not A. moyanoi, which is consistent with reports of toxicity regarding these species. The symbiont Alternaria section Undifilum was detected by PCR in all three species that contained swainsonine but not in A. moyanoi. A fungal symbiont was isolated from seeds of Astragalus pehuenches and A. illinii. The isolated symbiont from both respective species produced swainsonine in vitro, and was demonstrated to belong to the genus Alternaria section Undifilum by analysis of the nuclear ribosomal DNA. It is highly likely that Alternaria section Undifilum isolates will be associated with other South American Astragalus species that are reported to contain swainsonine.
Estación Experimental Agropecuaria Bariloche
Fil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina
Fil: Roper, Jessie M. USDA. ARS Poisonous Plant Research; Estados Unidos
Fil: Gardner, Dale R. USDA. ARS Poisonous Plant Research; Estados Unidos
Fil: Neyaz, Marwah S. New Mexico State University. Department of Entomology, Plant Pathology and Weed Science; Mexico
Fil: Joelson, Natalia Zoe. Universidad Nacional del Comahue; Argentina
Fil: Cook, Daniel. USDA. ARS Poisonous Plant Research; Estados Unidos
Fuente
Toxicon 171 (5) : 1-6 (Diciembre 2019)
Materia
Astragalus
Toxicidad
Toxicidad por Ingestión
Fitotoxicidad
Envenenamiento
Sanidad Animal
Toxicity
Ingestion Toxicity
Phytotoxicity
Poisoning
Animal Health
Swainsonine
Región Patagónica
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6518
Acceder
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spelling Detection of swainsonine-producing endophytes in Patagonian Astragalus speciesMartinez, AgustinRobles, Carlos AlejandroRoper, Jessie M.Gardner, Dale R.Neyaz, Marwah S.Joelson, Natalia ZoeCook, DanielAstragalusToxicidadToxicidad por IngestiónFitotoxicidadEnvenenamientoSanidad AnimalToxicityIngestion ToxicityPhytotoxicityPoisoningAnimal HealthSwainsonineRegión PatagónicaSwainsonine has been identified as the toxin in legumes belonging to the genera Astragalus and Oxytropis throughout the world including China, North America, and South America. Several South American Astragalus species have been reported to contain swainsonine; however, data is lacking to support the presence of a fungal symbiont in South American Astragalus species as has been shown for North American and Chinese Astragalus and Oxytropis species. The objective of this study was to investigate several South American species that have been reported to contain swainsonine for the presence of the fungal symbiont using culturing and PCR. Swainsonine was detected in field collections of A. pehuenches, A. illinii and A. chamissonis but not A. moyanoi, which is consistent with reports of toxicity regarding these species. The symbiont Alternaria section Undifilum was detected by PCR in all three species that contained swainsonine but not in A. moyanoi. A fungal symbiont was isolated from seeds of Astragalus pehuenches and A. illinii. The isolated symbiont from both respective species produced swainsonine in vitro, and was demonstrated to belong to the genus Alternaria section Undifilum by analysis of the nuclear ribosomal DNA. It is highly likely that Alternaria section Undifilum isolates will be associated with other South American Astragalus species that are reported to contain swainsonine.Estación Experimental Agropecuaria BarilocheFil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; ArgentinaFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; ArgentinaFil: Roper, Jessie M. USDA. ARS Poisonous Plant Research; Estados UnidosFil: Gardner, Dale R. USDA. ARS Poisonous Plant Research; Estados UnidosFil: Neyaz, Marwah S. New Mexico State University. Department of Entomology, Plant Pathology and Weed Science; MexicoFil: Joelson, Natalia Zoe. Universidad Nacional del Comahue; ArgentinaFil: Cook, Daniel. USDA. ARS Poisonous Plant Research; Estados UnidosElsevier2019-12-16T12:55:45Z2019-12-16T12:55:45Z2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6518https://www.sciencedirect.com/science/article/pii/S00410101193047020041-0101https://doi.org/10.1016/j.toxicon.2019.09.020Toxicon 171 (5) : 1-6 (Diciembre 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:10Zoai:localhost:20.500.12123/6518instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:10.804INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection of swainsonine-producing endophytes in Patagonian Astragalus species
title Detection of swainsonine-producing endophytes in Patagonian Astragalus species
spellingShingle Detection of swainsonine-producing endophytes in Patagonian Astragalus species
Martinez, Agustin
Astragalus
Toxicidad
Toxicidad por Ingestión
Fitotoxicidad
Envenenamiento
Sanidad Animal
Toxicity
Ingestion Toxicity
Phytotoxicity
Poisoning
Animal Health
Swainsonine
Región Patagónica
title_short Detection of swainsonine-producing endophytes in Patagonian Astragalus species
title_full Detection of swainsonine-producing endophytes in Patagonian Astragalus species
title_fullStr Detection of swainsonine-producing endophytes in Patagonian Astragalus species
title_full_unstemmed Detection of swainsonine-producing endophytes in Patagonian Astragalus species
title_sort Detection of swainsonine-producing endophytes in Patagonian Astragalus species
dc.creator.none.fl_str_mv Martinez, Agustin
Robles, Carlos Alejandro
Roper, Jessie M.
Gardner, Dale R.
Neyaz, Marwah S.
Joelson, Natalia Zoe
Cook, Daniel
author Martinez, Agustin
author_facet Martinez, Agustin
Robles, Carlos Alejandro
Roper, Jessie M.
Gardner, Dale R.
Neyaz, Marwah S.
Joelson, Natalia Zoe
Cook, Daniel
author_role author
author2 Robles, Carlos Alejandro
Roper, Jessie M.
Gardner, Dale R.
Neyaz, Marwah S.
Joelson, Natalia Zoe
Cook, Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Astragalus
Toxicidad
Toxicidad por Ingestión
Fitotoxicidad
Envenenamiento
Sanidad Animal
Toxicity
Ingestion Toxicity
Phytotoxicity
Poisoning
Animal Health
Swainsonine
Región Patagónica
topic Astragalus
Toxicidad
Toxicidad por Ingestión
Fitotoxicidad
Envenenamiento
Sanidad Animal
Toxicity
Ingestion Toxicity
Phytotoxicity
Poisoning
Animal Health
Swainsonine
Región Patagónica
dc.description.none.fl_txt_mv Swainsonine has been identified as the toxin in legumes belonging to the genera Astragalus and Oxytropis throughout the world including China, North America, and South America. Several South American Astragalus species have been reported to contain swainsonine; however, data is lacking to support the presence of a fungal symbiont in South American Astragalus species as has been shown for North American and Chinese Astragalus and Oxytropis species. The objective of this study was to investigate several South American species that have been reported to contain swainsonine for the presence of the fungal symbiont using culturing and PCR. Swainsonine was detected in field collections of A. pehuenches, A. illinii and A. chamissonis but not A. moyanoi, which is consistent with reports of toxicity regarding these species. The symbiont Alternaria section Undifilum was detected by PCR in all three species that contained swainsonine but not in A. moyanoi. A fungal symbiont was isolated from seeds of Astragalus pehuenches and A. illinii. The isolated symbiont from both respective species produced swainsonine in vitro, and was demonstrated to belong to the genus Alternaria section Undifilum by analysis of the nuclear ribosomal DNA. It is highly likely that Alternaria section Undifilum isolates will be associated with other South American Astragalus species that are reported to contain swainsonine.
Estación Experimental Agropecuaria Bariloche
Fil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina
Fil: Roper, Jessie M. USDA. ARS Poisonous Plant Research; Estados Unidos
Fil: Gardner, Dale R. USDA. ARS Poisonous Plant Research; Estados Unidos
Fil: Neyaz, Marwah S. New Mexico State University. Department of Entomology, Plant Pathology and Weed Science; Mexico
Fil: Joelson, Natalia Zoe. Universidad Nacional del Comahue; Argentina
Fil: Cook, Daniel. USDA. ARS Poisonous Plant Research; Estados Unidos
description Swainsonine has been identified as the toxin in legumes belonging to the genera Astragalus and Oxytropis throughout the world including China, North America, and South America. Several South American Astragalus species have been reported to contain swainsonine; however, data is lacking to support the presence of a fungal symbiont in South American Astragalus species as has been shown for North American and Chinese Astragalus and Oxytropis species. The objective of this study was to investigate several South American species that have been reported to contain swainsonine for the presence of the fungal symbiont using culturing and PCR. Swainsonine was detected in field collections of A. pehuenches, A. illinii and A. chamissonis but not A. moyanoi, which is consistent with reports of toxicity regarding these species. The symbiont Alternaria section Undifilum was detected by PCR in all three species that contained swainsonine but not in A. moyanoi. A fungal symbiont was isolated from seeds of Astragalus pehuenches and A. illinii. The isolated symbiont from both respective species produced swainsonine in vitro, and was demonstrated to belong to the genus Alternaria section Undifilum by analysis of the nuclear ribosomal DNA. It is highly likely that Alternaria section Undifilum isolates will be associated with other South American Astragalus species that are reported to contain swainsonine.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-16T12:55:45Z
2019-12-16T12:55:45Z
2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6518
https://www.sciencedirect.com/science/article/pii/S0041010119304702
0041-0101
https://doi.org/10.1016/j.toxicon.2019.09.020
url http://hdl.handle.net/20.500.12123/6518
https://www.sciencedirect.com/science/article/pii/S0041010119304702
https://doi.org/10.1016/j.toxicon.2019.09.020
identifier_str_mv 0041-0101
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Toxicon 171 (5) : 1-6 (Diciembre 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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