A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness

Autores
Bei, Jinlong; Bigi, María Mercedes; Lima, Analía; Zhang, Qi; Blanco, Federico Carlos; López, Beatriz; Yu, Ting; Wang, Zhilin; Dai, Zhangyan; Chen, Zhuang; Cataldi, Angel Adrian; Sasiain, María del Carmen; Ritacco, Viviana; De la Barrera, Silvia Susana; Soria, Marcelo Abel; Durán, Rosario; Bigi, Fabiana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.
Instituto de Biotecnología
Fil: Bei, Jinlong. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina
Fil: Lima, Analía. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Zhang, Qi. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: López, Beatriz. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; Argentina
Fil: Yu, Ting. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Wang, Zhilin. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Dai, Zhangyan. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Chen, Zhuang. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ritacco, Viviana. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; Argentina
Fil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos; Argentina
Fil: Durán, Rosario. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Biomed Research International 2020 : 4741237 (Abril 2020)
Materia
Genetic Isolation
Drug Resistance
Biological Properties
Aislamiento Genético
Resistencia a Medicamentos
Mycobacterium tuberculosis
Propiedades Biológicas
Molecular Properties
Propiedades Moleculares
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7980
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spelling A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitnessBei, JinlongBigi, María MercedesLima, AnalíaZhang, QiBlanco, Federico CarlosLópez, BeatrizYu, TingWang, ZhilinDai, ZhangyanChen, ZhuangCataldi, Angel AdrianSasiain, María del CarmenRitacco, VivianaDe la Barrera, Silvia SusanaSoria, Marcelo AbelDurán, RosarioBigi, FabianaGenetic IsolationDrug ResistanceBiological PropertiesAislamiento GenéticoResistencia a MedicamentosMycobacterium tuberculosisPropiedades BiológicasMolecular PropertiesPropiedades MolecularesTuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.Instituto de BiotecnologíaFil: Bei, Jinlong. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; ChinaFil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; ArgentinaFil: Lima, Analía. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; UruguayFil: Zhang, Qi. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; ChinaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: López, Beatriz. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; ArgentinaFil: Yu, Ting. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; ChinaFil: Wang, Zhilin. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; ChinaFil: Dai, Zhangyan. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; ChinaFil: Chen, Zhuang. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; ChinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Ritacco, Viviana. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; ArgentinaFil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos; ArgentinaFil: Durán, Rosario. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; UruguayFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaHindawi2020-09-28T16:37:46Z2020-09-28T16:37:46Z2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7980https://www.hindawi.com/journals/bmri/2020/4741237/2314-6141https://doi.org/10.1155/2020/4741237Biomed Research International 2020 : 4741237 (Abril 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:02Zoai:localhost:20.500.12123/7980instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:02.472INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
spellingShingle A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
Bei, Jinlong
Genetic Isolation
Drug Resistance
Biological Properties
Aislamiento Genético
Resistencia a Medicamentos
Mycobacterium tuberculosis
Propiedades Biológicas
Molecular Properties
Propiedades Moleculares
title_short A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_full A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_fullStr A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_full_unstemmed A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_sort A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
dc.creator.none.fl_str_mv Bei, Jinlong
Bigi, María Mercedes
Lima, Analía
Zhang, Qi
Blanco, Federico Carlos
López, Beatriz
Yu, Ting
Wang, Zhilin
Dai, Zhangyan
Chen, Zhuang
Cataldi, Angel Adrian
Sasiain, María del Carmen
Ritacco, Viviana
De la Barrera, Silvia Susana
Soria, Marcelo Abel
Durán, Rosario
Bigi, Fabiana
author Bei, Jinlong
author_facet Bei, Jinlong
Bigi, María Mercedes
Lima, Analía
Zhang, Qi
Blanco, Federico Carlos
López, Beatriz
Yu, Ting
Wang, Zhilin
Dai, Zhangyan
Chen, Zhuang
Cataldi, Angel Adrian
Sasiain, María del Carmen
Ritacco, Viviana
De la Barrera, Silvia Susana
Soria, Marcelo Abel
Durán, Rosario
Bigi, Fabiana
author_role author
author2 Bigi, María Mercedes
Lima, Analía
Zhang, Qi
Blanco, Federico Carlos
López, Beatriz
Yu, Ting
Wang, Zhilin
Dai, Zhangyan
Chen, Zhuang
Cataldi, Angel Adrian
Sasiain, María del Carmen
Ritacco, Viviana
De la Barrera, Silvia Susana
Soria, Marcelo Abel
Durán, Rosario
Bigi, Fabiana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genetic Isolation
Drug Resistance
Biological Properties
Aislamiento Genético
Resistencia a Medicamentos
Mycobacterium tuberculosis
Propiedades Biológicas
Molecular Properties
Propiedades Moleculares
topic Genetic Isolation
Drug Resistance
Biological Properties
Aislamiento Genético
Resistencia a Medicamentos
Mycobacterium tuberculosis
Propiedades Biológicas
Molecular Properties
Propiedades Moleculares
dc.description.none.fl_txt_mv Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.
Instituto de Biotecnología
Fil: Bei, Jinlong. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina
Fil: Lima, Analía. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Zhang, Qi. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: López, Beatriz. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; Argentina
Fil: Yu, Ting. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Wang, Zhilin. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Dai, Zhangyan. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Chen, Zhuang. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ritacco, Viviana. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; Argentina
Fil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos; Argentina
Fil: Durán, Rosario. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-28T16:37:46Z
2020-09-28T16:37:46Z
2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7980
https://www.hindawi.com/journals/bmri/2020/4741237/
2314-6141
https://doi.org/10.1155/2020/4741237
url http://hdl.handle.net/20.500.12123/7980
https://www.hindawi.com/journals/bmri/2020/4741237/
https://doi.org/10.1155/2020/4741237
identifier_str_mv 2314-6141
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv Biomed Research International 2020 : 4741237 (Abril 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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