A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness

Autores
Bei, Jinlong; Bigi, María Mercedes; Lima, Analía; Zhang, Qi; Blanco, Federico Carlos; Lopez, Beatriz; Yu, Ting; Wang, Zhilin; Dai, Zhangyan; Chen, Zhuang; Cataldi, Angel Adrian; Sasiain, María del Carmen; Ritacco, Gloria Viviana; de la Barrera, Silvia Susana; Soria, Marcelo Abel; Durán, Rosario; Bigi, Fabiana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.
Fil: Bei, Jinlong. Guangdong Academy of Agricultural Sciences; China
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Lima, Analía. Instituto Pasteur de Montevideo; Uruguay
Fil: Zhang, Qi. Guangdong Academy of Agricultural Sciences; China
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lopez, Beatriz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Yu, Ting. Guangdong Academy of Agricultural Sciences; China
Fil: Wang, Zhilin. Guangdong Academy of Agricultural Sciences; China
Fil: Dai, Zhangyan. Guangdong Academy of Agricultural Sciences; China
Fil: Chen, Zhuang. Guangdong Academy of Agricultural Sciences; China
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ritacco, Gloria Viviana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
Fil: Durán, Rosario. Instituto Pasteur de Montevideo; Uruguay
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Mycobacterium tuberculosis
MDR-TB
DevR
proteomics
mycolic acids
stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146502
Acceder
id CONICETDig_a1faa85decc3493b399dfd0b889f8ae3
oai_identifier_str oai:ri.conicet.gov.ar:11336/146502
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitnessBei, JinlongBigi, María MercedesLima, AnalíaZhang, QiBlanco, Federico CarlosLopez, BeatrizYu, TingWang, ZhilinDai, ZhangyanChen, ZhuangCataldi, Angel AdrianSasiain, María del CarmenRitacco, Gloria Vivianade la Barrera, Silvia SusanaSoria, Marcelo AbelDurán, RosarioBigi, FabianaMycobacterium tuberculosisMDR-TBDevRproteomicsmycolic acidsstresshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.Fil: Bei, Jinlong. Guangdong Academy of Agricultural Sciences; ChinaFil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Lima, Analía. Instituto Pasteur de Montevideo; UruguayFil: Zhang, Qi. Guangdong Academy of Agricultural Sciences; ChinaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lopez, Beatriz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Yu, Ting. Guangdong Academy of Agricultural Sciences; ChinaFil: Wang, Zhilin. Guangdong Academy of Agricultural Sciences; ChinaFil: Dai, Zhangyan. Guangdong Academy of Agricultural Sciences; ChinaFil: Chen, Zhuang. Guangdong Academy of Agricultural Sciences; ChinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Ritacco, Gloria Viviana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; ArgentinaFil: Durán, Rosario. Instituto Pasteur de Montevideo; UruguayFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaHindawi Limited2020-04-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146502Bei, Jinlong; Bigi, María Mercedes; Lima, Analía; Zhang, Qi; Blanco, Federico Carlos; et al.; A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness; Hindawi Limited; BioMed Research International; 2020; 8-4-2020; 1-92314-61332314-6141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2020/4741237/info:eu-repo/semantics/altIdentifier/doi/10.1155/2020/4741237info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:11Zoai:ri.conicet.gov.ar:11336/146502instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:12.228CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
spellingShingle A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
Bei, Jinlong
Mycobacterium tuberculosis
MDR-TB
DevR
proteomics
mycolic acids
stress
title_short A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_full A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_fullStr A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_full_unstemmed A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
title_sort A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
dc.creator.none.fl_str_mv Bei, Jinlong
Bigi, María Mercedes
Lima, Analía
Zhang, Qi
Blanco, Federico Carlos
Lopez, Beatriz
Yu, Ting
Wang, Zhilin
Dai, Zhangyan
Chen, Zhuang
Cataldi, Angel Adrian
Sasiain, María del Carmen
Ritacco, Gloria Viviana
de la Barrera, Silvia Susana
Soria, Marcelo Abel
Durán, Rosario
Bigi, Fabiana
author Bei, Jinlong
author_facet Bei, Jinlong
Bigi, María Mercedes
Lima, Analía
Zhang, Qi
Blanco, Federico Carlos
Lopez, Beatriz
Yu, Ting
Wang, Zhilin
Dai, Zhangyan
Chen, Zhuang
Cataldi, Angel Adrian
Sasiain, María del Carmen
Ritacco, Gloria Viviana
de la Barrera, Silvia Susana
Soria, Marcelo Abel
Durán, Rosario
Bigi, Fabiana
author_role author
author2 Bigi, María Mercedes
Lima, Analía
Zhang, Qi
Blanco, Federico Carlos
Lopez, Beatriz
Yu, Ting
Wang, Zhilin
Dai, Zhangyan
Chen, Zhuang
Cataldi, Angel Adrian
Sasiain, María del Carmen
Ritacco, Gloria Viviana
de la Barrera, Silvia Susana
Soria, Marcelo Abel
Durán, Rosario
Bigi, Fabiana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium tuberculosis
MDR-TB
DevR
proteomics
mycolic acids
stress
topic Mycobacterium tuberculosis
MDR-TB
DevR
proteomics
mycolic acids
stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.
Fil: Bei, Jinlong. Guangdong Academy of Agricultural Sciences; China
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Lima, Analía. Instituto Pasteur de Montevideo; Uruguay
Fil: Zhang, Qi. Guangdong Academy of Agricultural Sciences; China
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lopez, Beatriz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Yu, Ting. Guangdong Academy of Agricultural Sciences; China
Fil: Wang, Zhilin. Guangdong Academy of Agricultural Sciences; China
Fil: Dai, Zhangyan. Guangdong Academy of Agricultural Sciences; China
Fil: Chen, Zhuang. Guangdong Academy of Agricultural Sciences; China
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ritacco, Gloria Viviana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
Fil: Durán, Rosario. Instituto Pasteur de Montevideo; Uruguay
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146502
Bei, Jinlong; Bigi, María Mercedes; Lima, Analía; Zhang, Qi; Blanco, Federico Carlos; et al.; A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness; Hindawi Limited; BioMed Research International; 2020; 8-4-2020; 1-9
2314-6133
2314-6141
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146502
identifier_str_mv Bei, Jinlong; Bigi, María Mercedes; Lima, Analía; Zhang, Qi; Blanco, Federico Carlos; et al.; A Phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness; Hindawi Limited; BioMed Research International; 2020; 8-4-2020; 1-9
2314-6133
2314-6141
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2020/4741237/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2020/4741237
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Limited
publisher.none.fl_str_mv Hindawi Limited
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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