Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato

Autores
Nahirñak, Vanesa; Rivarola, Maximo Lisandro; Almasia, Natalia Ines; Barrios Baron, María Pilar; Hopp, Horacio Esteban; Vile, Denis; Paniego, Norma Beatriz; Vazquez Rovere, Cecilia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.
Instituto de Biotecnología
Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Barrios Barón, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Vile, Denis. Institute National de la Recherche Agronomique. Laboratoire d'écophysiologie des Plantes sous Stress Environnementaux; Francia; Université de Montpellier; Francia
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA).Labintex Europa; Francia
Fuente
PLoS ONE 14 (3) : e0214165. (March 25, 2019)
Materia
Papa
Solanum Tuberosum
Acido Ascórbico
Hormonas
Esteroles
Metabolismo
Oxirredución
Especies de Oxígeno Reactivo
Potatoes
Ascorbic Acid
Hormones
Sterols
Metabolism
Oxidoreductions
Reactive Oxygen Species
Snakin-1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4762
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oai_identifier_str oai:localhost:20.500.12123/4762
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network_name_str INTA Digital (INTA)
spelling Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potatoNahirñak, VanesaRivarola, Maximo LisandroAlmasia, Natalia InesBarrios Baron, María PilarHopp, Horacio EstebanVile, DenisPaniego, Norma BeatrizVazquez Rovere, CeciliaPapaSolanum TuberosumAcido AscórbicoHormonasEsterolesMetabolismoOxirreduciónEspecies de Oxígeno ReactivoPotatoesAscorbic AcidHormonesSterolsMetabolismOxidoreductionsReactive Oxygen SpeciesSnakin-1Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.Instituto de BiotecnologíaFil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Barrios Barón, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Vile, Denis. Institute National de la Recherche Agronomique. Laboratoire d'écophysiologie des Plantes sous Stress Environnementaux; Francia; Université de Montpellier; FranciaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA).Labintex Europa; FranciaPLOS2019-03-27T16:39:50Z2019-03-27T16:39:50Z2019-03-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4762https://journals.plos.org/plosone/article?id=10.1371/journal.pone.02141651932-6203https://doi.org/10.1371/journal.pone.0214165PLoS ONE 14 (3) : e0214165. (March 25, 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:54Zoai:localhost:20.500.12123/4762instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:54.462INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
title Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
spellingShingle Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
Nahirñak, Vanesa
Papa
Solanum Tuberosum
Acido Ascórbico
Hormonas
Esteroles
Metabolismo
Oxirredución
Especies de Oxígeno Reactivo
Potatoes
Ascorbic Acid
Hormones
Sterols
Metabolism
Oxidoreductions
Reactive Oxygen Species
Snakin-1
title_short Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
title_full Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
title_fullStr Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
title_full_unstemmed Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
title_sort Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
dc.creator.none.fl_str_mv Nahirñak, Vanesa
Rivarola, Maximo Lisandro
Almasia, Natalia Ines
Barrios Baron, María Pilar
Hopp, Horacio Esteban
Vile, Denis
Paniego, Norma Beatriz
Vazquez Rovere, Cecilia
author Nahirñak, Vanesa
author_facet Nahirñak, Vanesa
Rivarola, Maximo Lisandro
Almasia, Natalia Ines
Barrios Baron, María Pilar
Hopp, Horacio Esteban
Vile, Denis
Paniego, Norma Beatriz
Vazquez Rovere, Cecilia
author_role author
author2 Rivarola, Maximo Lisandro
Almasia, Natalia Ines
Barrios Baron, María Pilar
Hopp, Horacio Esteban
Vile, Denis
Paniego, Norma Beatriz
Vazquez Rovere, Cecilia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Papa
Solanum Tuberosum
Acido Ascórbico
Hormonas
Esteroles
Metabolismo
Oxirredución
Especies de Oxígeno Reactivo
Potatoes
Ascorbic Acid
Hormones
Sterols
Metabolism
Oxidoreductions
Reactive Oxygen Species
Snakin-1
topic Papa
Solanum Tuberosum
Acido Ascórbico
Hormonas
Esteroles
Metabolismo
Oxirredución
Especies de Oxígeno Reactivo
Potatoes
Ascorbic Acid
Hormones
Sterols
Metabolism
Oxidoreductions
Reactive Oxygen Species
Snakin-1
dc.description.none.fl_txt_mv Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.
Instituto de Biotecnología
Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Barrios Barón, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Vile, Denis. Institute National de la Recherche Agronomique. Laboratoire d'écophysiologie des Plantes sous Stress Environnementaux; Francia; Université de Montpellier; Francia
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA).Labintex Europa; Francia
description Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-27T16:39:50Z
2019-03-27T16:39:50Z
2019-03-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4762
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214165
1932-6203
https://doi.org/10.1371/journal.pone.0214165
url http://hdl.handle.net/20.500.12123/4762
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214165
https://doi.org/10.1371/journal.pone.0214165
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv PLoS ONE 14 (3) : e0214165. (March 25, 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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