Subfunctionalization probably drives the emergence of plant growth‑promoting genes

Autores
Frare, Romina Alejandra; Mozzicafreddo, Matteo; Gomez, Maria Cristina; Fussoni, Nerina Belen; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence.
Instituto de Biotecnología
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; Italia
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
Symbiosis 87 (3) : 249-253 (Julio 2022)
Materia
Azospirillum brasilense
Rizosfera
Medicago sativa
Crecimiento de Planta
Rhizosphere
Plant Growth
Promoting Genes
Genes Promotores
Alfalfa
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15664
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oai_identifier_str oai:localhost:20.500.12123/15664
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Subfunctionalization probably drives the emergence of plant growth‑promoting genesFrare, Romina AlejandraMozzicafreddo, MatteoGomez, Maria CristinaFussoni, Nerina BelenSoto, Gabriela CynthiaAyub, Nicolás DanielAzospirillum brasilenseRizosferaMedicago sativaCrecimiento de PlantaRhizospherePlant GrowthPromoting GenesGenes PromotoresAlfalfaThe duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence.Instituto de BiotecnologíaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; ItaliaFil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2023-10-24T16:54:26Z2023-10-24T16:54:26Z2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15664https://link.springer.com/article/10.1007/s13199-022-00872-x1878-7665https://doi.org/10.1007/s13199-022-00872-xSymbiosis 87 (3) : 249-253 (Julio 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:20Zoai:localhost:20.500.12123/15664instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:21.188INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Subfunctionalization probably drives the emergence of plant growth‑promoting genes
title Subfunctionalization probably drives the emergence of plant growth‑promoting genes
spellingShingle Subfunctionalization probably drives the emergence of plant growth‑promoting genes
Frare, Romina Alejandra
Azospirillum brasilense
Rizosfera
Medicago sativa
Crecimiento de Planta
Rhizosphere
Plant Growth
Promoting Genes
Genes Promotores
Alfalfa
title_short Subfunctionalization probably drives the emergence of plant growth‑promoting genes
title_full Subfunctionalization probably drives the emergence of plant growth‑promoting genes
title_fullStr Subfunctionalization probably drives the emergence of plant growth‑promoting genes
title_full_unstemmed Subfunctionalization probably drives the emergence of plant growth‑promoting genes
title_sort Subfunctionalization probably drives the emergence of plant growth‑promoting genes
dc.creator.none.fl_str_mv Frare, Romina Alejandra
Mozzicafreddo, Matteo
Gomez, Maria Cristina
Fussoni, Nerina Belen
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author Frare, Romina Alejandra
author_facet Frare, Romina Alejandra
Mozzicafreddo, Matteo
Gomez, Maria Cristina
Fussoni, Nerina Belen
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author_role author
author2 Mozzicafreddo, Matteo
Gomez, Maria Cristina
Fussoni, Nerina Belen
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Azospirillum brasilense
Rizosfera
Medicago sativa
Crecimiento de Planta
Rhizosphere
Plant Growth
Promoting Genes
Genes Promotores
Alfalfa
topic Azospirillum brasilense
Rizosfera
Medicago sativa
Crecimiento de Planta
Rhizosphere
Plant Growth
Promoting Genes
Genes Promotores
Alfalfa
dc.description.none.fl_txt_mv The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence.
Instituto de Biotecnología
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; Italia
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence.
publishDate 2022
dc.date.none.fl_str_mv 2022-07
2023-10-24T16:54:26Z
2023-10-24T16:54:26Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15664
https://link.springer.com/article/10.1007/s13199-022-00872-x
1878-7665
https://doi.org/10.1007/s13199-022-00872-x
url http://hdl.handle.net/20.500.12123/15664
https://link.springer.com/article/10.1007/s13199-022-00872-x
https://doi.org/10.1007/s13199-022-00872-x
identifier_str_mv 1878-7665
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Symbiosis 87 (3) : 249-253 (Julio 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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