Subfunctionalization probably drives the emergence of plant growth‑promoting genes
- Autores
- Frare, Romina Alejandra; Mozzicafreddo, Matteo; Gomez, Maria Cristina; Fussoni, Nerina Belen; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence.
Instituto de Biotecnología
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; Italia
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina - Fuente
- Symbiosis 87 (3) : 249-253 (Julio 2022)
- Materia
-
Azospirillum brasilense
Rizosfera
Medicago sativa
Crecimiento de Planta
Rhizosphere
Plant Growth
Promoting Genes
Genes Promotores
Alfalfa - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/15664
Ver los metadatos del registro completo
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Subfunctionalization probably drives the emergence of plant growth‑promoting genesFrare, Romina AlejandraMozzicafreddo, MatteoGomez, Maria CristinaFussoni, Nerina BelenSoto, Gabriela CynthiaAyub, Nicolás DanielAzospirillum brasilenseRizosferaMedicago sativaCrecimiento de PlantaRhizospherePlant GrowthPromoting GenesGenes PromotoresAlfalfaThe duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence.Instituto de BiotecnologíaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; ItaliaFil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2023-10-24T16:54:26Z2023-10-24T16:54:26Z2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15664https://link.springer.com/article/10.1007/s13199-022-00872-x1878-7665https://doi.org/10.1007/s13199-022-00872-xSymbiosis 87 (3) : 249-253 (Julio 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:10Zoai:localhost:20.500.12123/15664instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:11.06INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
title |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
spellingShingle |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes Frare, Romina Alejandra Azospirillum brasilense Rizosfera Medicago sativa Crecimiento de Planta Rhizosphere Plant Growth Promoting Genes Genes Promotores Alfalfa |
title_short |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
title_full |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
title_fullStr |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
title_full_unstemmed |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
title_sort |
Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
dc.creator.none.fl_str_mv |
Frare, Romina Alejandra Mozzicafreddo, Matteo Gomez, Maria Cristina Fussoni, Nerina Belen Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author |
Frare, Romina Alejandra |
author_facet |
Frare, Romina Alejandra Mozzicafreddo, Matteo Gomez, Maria Cristina Fussoni, Nerina Belen Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author_role |
author |
author2 |
Mozzicafreddo, Matteo Gomez, Maria Cristina Fussoni, Nerina Belen Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Azospirillum brasilense Rizosfera Medicago sativa Crecimiento de Planta Rhizosphere Plant Growth Promoting Genes Genes Promotores Alfalfa |
topic |
Azospirillum brasilense Rizosfera Medicago sativa Crecimiento de Planta Rhizosphere Plant Growth Promoting Genes Genes Promotores Alfalfa |
dc.description.none.fl_txt_mv |
The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence. Instituto de Biotecnología Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; Italia Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina |
description |
The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07 2023-10-24T16:54:26Z 2023-10-24T16:54:26Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/15664 https://link.springer.com/article/10.1007/s13199-022-00872-x 1878-7665 https://doi.org/10.1007/s13199-022-00872-x |
url |
http://hdl.handle.net/20.500.12123/15664 https://link.springer.com/article/10.1007/s13199-022-00872-x https://doi.org/10.1007/s13199-022-00872-x |
identifier_str_mv |
1878-7665 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Symbiosis 87 (3) : 249-253 (Julio 2022) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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