Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles

Autores
Poulsen Hornum, Anabela; Camadro, Elsa Lucila
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25 plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and cross-incompatibility) and post-zygotic (male sterility and inviability and weakness of F1 hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises.
EEA Balcarce
Fil: Poulsen Hornum, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Genetic Resources and Crop Evolution 68 : 915–938 (2021)
Materia
Papa
Potatoes
Solanum Chacoense
Banco de Germoplasma
Germplasm Banks
Varieties
Variedades
Papa Silvestre
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8762
Acceder
id INTADig_eb438d31b3fe64f39c0a050a9b522403
oai_identifier_str oai:localhost:20.500.12123/8762
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cyclesPoulsen Hornum, AnabelaCamadro, Elsa LucilaPapaPotatoesSolanum ChacoenseBanco de GermoplasmaGermplasm BanksVarietiesVariedadesPapa SilvestreWild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25 plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and cross-incompatibility) and post-zygotic (male sterility and inviability and weakness of F1 hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises.EEA BalcarceFil: Poulsen Hornum, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer Nature2021-02-26T13:14:42Z2021-02-26T13:14:42Z2020-10-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8762https://link.springer.com/article/10.1007/s10722-020-01034-y1573-5109 (online)0925-9864 (print)https://doi.org/10.1007/s10722-020-01034-yGenetic Resources and Crop Evolution 68 : 915–938 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:46Zoai:localhost:20.500.12123/8762instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:47.081INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
title Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
spellingShingle Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
Poulsen Hornum, Anabela
Papa
Potatoes
Solanum Chacoense
Banco de Germoplasma
Germplasm Banks
Varieties
Variedades
Papa Silvestre
title_short Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
title_full Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
title_fullStr Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
title_full_unstemmed Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
title_sort Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
dc.creator.none.fl_str_mv Poulsen Hornum, Anabela
Camadro, Elsa Lucila
author Poulsen Hornum, Anabela
author_facet Poulsen Hornum, Anabela
Camadro, Elsa Lucila
author_role author
author2 Camadro, Elsa Lucila
author2_role author
dc.subject.none.fl_str_mv Papa
Potatoes
Solanum Chacoense
Banco de Germoplasma
Germplasm Banks
Varieties
Variedades
Papa Silvestre
topic Papa
Potatoes
Solanum Chacoense
Banco de Germoplasma
Germplasm Banks
Varieties
Variedades
Papa Silvestre
dc.description.none.fl_txt_mv Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25 plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and cross-incompatibility) and post-zygotic (male sterility and inviability and weakness of F1 hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises.
EEA Balcarce
Fil: Poulsen Hornum, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25 plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and cross-incompatibility) and post-zygotic (male sterility and inviability and weakness of F1 hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-07
2021-02-26T13:14:42Z
2021-02-26T13:14:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8762
https://link.springer.com/article/10.1007/s10722-020-01034-y
1573-5109 (online)
0925-9864 (print)
https://doi.org/10.1007/s10722-020-01034-y
url http://hdl.handle.net/20.500.12123/8762
https://link.springer.com/article/10.1007/s10722-020-01034-y
https://doi.org/10.1007/s10722-020-01034-y
identifier_str_mv 1573-5109 (online)
0925-9864 (print)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Genetic Resources and Crop Evolution 68 : 915–938 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341384945664000
score 12.623145

Publicaciones similares