Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells

Autores
Berretta, Marcelo Facundo; Lopez, Maria Gabriela; Taboga, Oscar Alberto; Sciocco, Alicia Ines; Romanowski, Victor
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.
Instituto de Microbiología y Zoología Agrícola
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Romanowski, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fuente
Virus Genes 46 (1) : 152–161 (February 2013)
Materia
Spodoptera frugiperda
Lepidoptera
Expresión Génica
Baculovirus
Gene Expression
Nucleopolyhedrovirus
Sf9 Cells
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3598
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spelling Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cellsBerretta, Marcelo FacundoLopez, Maria GabrielaTaboga, Oscar AlbertoSciocco, Alicia InesRomanowski, VictorSpodoptera frugiperdaLepidopteraExpresión GénicaBaculovirusGene ExpressionNucleopolyhedrovirusSf9 CellsWe used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.Instituto de Microbiología y Zoología AgrícolaFil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Romanowski, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina2018-10-16T12:55:12Z2018-10-16T12:55:12Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007%2Fs11262-012-0843-5http://hdl.handle.net/20.500.12123/35980920-85691572-994Xhttps://doi.org/10.1007/s11262-012-0843-5Virus Genes 46 (1) : 152–161 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:37Zoai:localhost:20.500.12123/3598instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:37.878INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
title Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
spellingShingle Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
Berretta, Marcelo Facundo
Spodoptera frugiperda
Lepidoptera
Expresión Génica
Baculovirus
Gene Expression
Nucleopolyhedrovirus
Sf9 Cells
title_short Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
title_full Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
title_fullStr Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
title_full_unstemmed Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
title_sort Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
dc.creator.none.fl_str_mv Berretta, Marcelo Facundo
Lopez, Maria Gabriela
Taboga, Oscar Alberto
Sciocco, Alicia Ines
Romanowski, Victor
author Berretta, Marcelo Facundo
author_facet Berretta, Marcelo Facundo
Lopez, Maria Gabriela
Taboga, Oscar Alberto
Sciocco, Alicia Ines
Romanowski, Victor
author_role author
author2 Lopez, Maria Gabriela
Taboga, Oscar Alberto
Sciocco, Alicia Ines
Romanowski, Victor
author2_role author
author
author
author
dc.subject.none.fl_str_mv Spodoptera frugiperda
Lepidoptera
Expresión Génica
Baculovirus
Gene Expression
Nucleopolyhedrovirus
Sf9 Cells
topic Spodoptera frugiperda
Lepidoptera
Expresión Génica
Baculovirus
Gene Expression
Nucleopolyhedrovirus
Sf9 Cells
dc.description.none.fl_txt_mv We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.
Instituto de Microbiología y Zoología Agrícola
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Romanowski, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
2018-10-16T12:55:12Z
2018-10-16T12:55:12Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007%2Fs11262-012-0843-5
http://hdl.handle.net/20.500.12123/3598
0920-8569
1572-994X
https://doi.org/10.1007/s11262-012-0843-5
url https://link.springer.com/article/10.1007%2Fs11262-012-0843-5
http://hdl.handle.net/20.500.12123/3598
https://doi.org/10.1007/s11262-012-0843-5
identifier_str_mv 0920-8569
1572-994X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Virus Genes 46 (1) : 152–161 (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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