Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus

Autores
Masson, Tomás; Fabre, María Laura; Pidre, Matias Luis; Niz, José María; Berretta, Marcelo Facundo; Romanowski, Víctor; Ferrelli, María Leticia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. To date, SfMNPV genomic diversity of different isolates has been mainly studied by means of restriction pattern analyses and by sequencing of the egt region. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Masson, Tomás. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Fabre, María Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Pidre, Matias Luis. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Romanowski, Víctor. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Ferrelli, María Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fuente
Infection, Genetics and Evolution 90 : 104749 (junio 2021)
Materia
Spodoptera frugiperda
Control Biológico
Biological Control
Pesticides
Plaguicidas
Baculovirus
Genomic Diversity
Virus Evolution
SfMNPV
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12408
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oai_identifier_str oai:localhost:20.500.12123/12408
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network_name_str INTA Digital (INTA)
spelling Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirusMasson, TomásFabre, María LauraPidre, Matias LuisNiz, José MaríaBerretta, Marcelo FacundoRomanowski, VíctorFerrelli, María LeticiaSpodoptera frugiperdaControl BiológicoBiological ControlPesticidesPlaguicidasBaculovirusGenomic DiversityVirus EvolutionSfMNPVSpodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. To date, SfMNPV genomic diversity of different isolates has been mainly studied by means of restriction pattern analyses and by sequencing of the egt region. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Masson, Tomás. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); ArgentinaFil: Fabre, María Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); ArgentinaFil: Pidre, Matias Luis. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); ArgentinaFil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Romanowski, Víctor. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); ArgentinaFil: Ferrelli, María Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); ArgentinaElsevier2022-07-26T22:16:56Z2022-07-26T22:16:56Z2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12408https://www.sciencedirect.com/science/article/abs/pii/S1567134821000460https://doi.org/10.1016/j.meegid.2021.104749Infection, Genetics and Evolution 90 : 104749 (junio 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-18T10:08:40Zoai:localhost:20.500.12123/12408instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:08:40.874INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
title Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
spellingShingle Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
Masson, Tomás
Spodoptera frugiperda
Control Biológico
Biological Control
Pesticides
Plaguicidas
Baculovirus
Genomic Diversity
Virus Evolution
SfMNPV
title_short Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
title_full Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
title_fullStr Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
title_full_unstemmed Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
title_sort Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
dc.creator.none.fl_str_mv Masson, Tomás
Fabre, María Laura
Pidre, Matias Luis
Niz, José María
Berretta, Marcelo Facundo
Romanowski, Víctor
Ferrelli, María Leticia
author Masson, Tomás
author_facet Masson, Tomás
Fabre, María Laura
Pidre, Matias Luis
Niz, José María
Berretta, Marcelo Facundo
Romanowski, Víctor
Ferrelli, María Leticia
author_role author
author2 Fabre, María Laura
Pidre, Matias Luis
Niz, José María
Berretta, Marcelo Facundo
Romanowski, Víctor
Ferrelli, María Leticia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Spodoptera frugiperda
Control Biológico
Biological Control
Pesticides
Plaguicidas
Baculovirus
Genomic Diversity
Virus Evolution
SfMNPV
topic Spodoptera frugiperda
Control Biológico
Biological Control
Pesticides
Plaguicidas
Baculovirus
Genomic Diversity
Virus Evolution
SfMNPV
dc.description.none.fl_txt_mv Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. To date, SfMNPV genomic diversity of different isolates has been mainly studied by means of restriction pattern analyses and by sequencing of the egt region. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Masson, Tomás. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Fabre, María Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Pidre, Matias Luis. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Romanowski, Víctor. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
Fil: Ferrelli, María Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina
description Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. To date, SfMNPV genomic diversity of different isolates has been mainly studied by means of restriction pattern analyses and by sequencing of the egt region. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
2022-07-26T22:16:56Z
2022-07-26T22:16:56Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12408
https://www.sciencedirect.com/science/article/abs/pii/S1567134821000460
https://doi.org/10.1016/j.meegid.2021.104749
url http://hdl.handle.net/20.500.12123/12408
https://www.sciencedirect.com/science/article/abs/pii/S1567134821000460
https://doi.org/10.1016/j.meegid.2021.104749
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Infection, Genetics and Evolution 90 : 104749 (junio 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.001348

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