An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi

Autores
Estrada-Navarrete, Georgina; Cruz-Mireles, Neftaly; Lascano, Hernan Ramiro; Alvarado-Affantranger, Xóchitl; Hernández-Barrera, Alejandra; Barraza, Aarón; Olivares, Juan E.; Arthikala, Manoj-Kumar; Cárdenas, Luis; Quinto, Carmen; Sanchez, Federico
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Cruz-Mireles, Neftaly. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina
Fil: Alvarado-Affantranger, Xóchitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Laboratorio Nacional de Microscopía Avanzada; México
Fil: Hernández-Barrera, Alejandra. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Barraza, Aarón. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Olivares, Juan E. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Arthikala, Manoj-Kumar. Universidad Nacional Autónoma de México. Escuela Nacional de Estudios Superiores-Unidad León; México
Fil: Cárdenas, Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Quinto, Carmen. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fuente
The Plant Cell 28 (9) : 2326-2341(September 2016)
Materia
Phaseolus Vulgaris
Rhizobiaceae
Arbuscular Mycorrhiza
Kinases
Micorrizas Arbusculares
Quinasas
Poroto
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10954
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10954
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal FungiEstrada-Navarrete, GeorginaCruz-Mireles, NeftalyLascano, Hernan RamiroAlvarado-Affantranger, XóchitlHernández-Barrera, AlejandraBarraza, AarónOlivares, Juan E.Arthikala, Manoj-KumarCárdenas, LuisQuinto, CarmenSanchez, FedericoPhaseolus VulgarisRhizobiaceaeArbuscular MycorrhizaKinasesMicorrizas ArbuscularesQuinasasPorotoEukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganismsInstituto de Fisiología y Recursos Genéticos VegetalesFil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Cruz-Mireles, Neftaly. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. ArgentinaFil: Alvarado-Affantranger, Xóchitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Laboratorio Nacional de Microscopía Avanzada; MéxicoFil: Hernández-Barrera, Alejandra. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Barraza, Aarón. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Olivares, Juan E. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Arthikala, Manoj-Kumar. Universidad Nacional Autónoma de México. Escuela Nacional de Estudios Superiores-Unidad León; MéxicoFil: Cárdenas, Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Quinto, Carmen. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoAmerican Society of Plant Biologists2021-12-21T10:46:52Z2021-12-21T10:46:52Z2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10954https://academic.oup.com/plcell/article/28/9/2326/60983871040-46511532-298X (online)https://doi.org/10.1105/tpc.15.01012The Plant Cell 28 (9) : 2326-2341(September 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:13Zoai:localhost:20.500.12123/10954instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:14.189INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
title An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
spellingShingle An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
Estrada-Navarrete, Georgina
Phaseolus Vulgaris
Rhizobiaceae
Arbuscular Mycorrhiza
Kinases
Micorrizas Arbusculares
Quinasas
Poroto
title_short An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
title_full An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
title_fullStr An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
title_full_unstemmed An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
title_sort An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
dc.creator.none.fl_str_mv Estrada-Navarrete, Georgina
Cruz-Mireles, Neftaly
Lascano, Hernan Ramiro
Alvarado-Affantranger, Xóchitl
Hernández-Barrera, Alejandra
Barraza, Aarón
Olivares, Juan E.
Arthikala, Manoj-Kumar
Cárdenas, Luis
Quinto, Carmen
Sanchez, Federico
author Estrada-Navarrete, Georgina
author_facet Estrada-Navarrete, Georgina
Cruz-Mireles, Neftaly
Lascano, Hernan Ramiro
Alvarado-Affantranger, Xóchitl
Hernández-Barrera, Alejandra
Barraza, Aarón
Olivares, Juan E.
Arthikala, Manoj-Kumar
Cárdenas, Luis
Quinto, Carmen
Sanchez, Federico
author_role author
author2 Cruz-Mireles, Neftaly
Lascano, Hernan Ramiro
Alvarado-Affantranger, Xóchitl
Hernández-Barrera, Alejandra
Barraza, Aarón
Olivares, Juan E.
Arthikala, Manoj-Kumar
Cárdenas, Luis
Quinto, Carmen
Sanchez, Federico
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Phaseolus Vulgaris
Rhizobiaceae
Arbuscular Mycorrhiza
Kinases
Micorrizas Arbusculares
Quinasas
Poroto
topic Phaseolus Vulgaris
Rhizobiaceae
Arbuscular Mycorrhiza
Kinases
Micorrizas Arbusculares
Quinasas
Poroto
dc.description.none.fl_txt_mv Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Cruz-Mireles, Neftaly. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina
Fil: Alvarado-Affantranger, Xóchitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Laboratorio Nacional de Microscopía Avanzada; México
Fil: Hernández-Barrera, Alejandra. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Barraza, Aarón. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Olivares, Juan E. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Arthikala, Manoj-Kumar. Universidad Nacional Autónoma de México. Escuela Nacional de Estudios Superiores-Unidad León; México
Fil: Cárdenas, Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Quinto, Carmen. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
description Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms
publishDate 2016
dc.date.none.fl_str_mv 2016-09
2021-12-21T10:46:52Z
2021-12-21T10:46:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10954
https://academic.oup.com/plcell/article/28/9/2326/6098387
1040-4651
1532-298X (online)
https://doi.org/10.1105/tpc.15.01012
url http://hdl.handle.net/20.500.12123/10954
https://academic.oup.com/plcell/article/28/9/2326/6098387
https://doi.org/10.1105/tpc.15.01012
identifier_str_mv 1040-4651
1532-298X (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologists
publisher.none.fl_str_mv American Society of Plant Biologists
dc.source.none.fl_str_mv The Plant Cell 28 (9) : 2326-2341(September 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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