First report of Anaplasma marginale infection in goats, Brazil

Autores
da Silva, Nayara B.; Taus, Naomi S.; Johnson, Wendell C.; Mira, Anabela; Schnittger, Leonhard; Valente, Jessica D. M.; Vidotto, Odilon; Masterson, Hayley E.; Vieira, Thállitha S. W. J.; Ueti, Massaro W.; Vieira, Rafael F. C.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.
Instituto de Patobiología
Fil: da Silva, Nayara B. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
Fil: Taus, Naomi S. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Johnson, Wendell C. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos
Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Valente, Jessica D. M. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
Fil: Vidotto, Odilon. Universidade Estadual de Londrina. Department of Preventive Veterinary Medicine; Brasil
Fil: Masterson, Hayley E. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Vieira, Thállitha S. W. J. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
Fil: Ueti, Massaro W. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Vieira, Rafael F. C. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
Fuente
PLoS One 13 (8) : e0202140 (Agosto 2018)
Materia
Anaplasma marginale
Caprinos
Brasil
Goats
Brazil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3776
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3776
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network_name_str INTA Digital (INTA)
spelling First report of Anaplasma marginale infection in goats, Brazilda Silva, Nayara B.Taus, Naomi S.Johnson, Wendell C.Mira, AnabelaSchnittger, LeonhardValente, Jessica D. M.Vidotto, OdilonMasterson, Hayley E.Vieira, Thállitha S. W. J.Ueti, Massaro W.Vieira, Rafael F. C.Anaplasma marginaleCaprinosBrasilGoatsBrazilAnaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.Instituto de PatobiologíaFil: da Silva, Nayara B. Universidade Federal do Parana. Department of Veterinary Medicine; BrasilFil: Taus, Naomi S. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos. Washington State University. Department of Veterinary Microbiology and Pathology; Estados UnidosFil: Johnson, Wendell C. United States Department of Agriculture. Animal Diseases Research Unit; Estados UnidosFil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Valente, Jessica D. M. Universidade Federal do Parana. Department of Veterinary Medicine; BrasilFil: Vidotto, Odilon. Universidade Estadual de Londrina. Department of Preventive Veterinary Medicine; BrasilFil: Masterson, Hayley E. Washington State University. Department of Veterinary Microbiology and Pathology; Estados UnidosFil: Vieira, Thállitha S. W. J. Universidade Federal do Parana. Department of Veterinary Medicine; BrasilFil: Ueti, Massaro W. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos. Washington State University. Department of Veterinary Microbiology and Pathology; Estados UnidosFil: Vieira, Rafael F. C. Universidade Federal do Parana. Department of Veterinary Medicine; BrasilPublic Library of Science2018-11-05T11:19:11Z2018-11-05T11:19:11Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202140http://hdl.handle.net/20.500.12123/37761932-6203https://doi.org/10.1371/journal.pone.0202140PLoS One 13 (8) : e0202140 (Agosto 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:38Zoai:localhost:20.500.12123/3776instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:39.72INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First report of Anaplasma marginale infection in goats, Brazil
title First report of Anaplasma marginale infection in goats, Brazil
spellingShingle First report of Anaplasma marginale infection in goats, Brazil
da Silva, Nayara B.
Anaplasma marginale
Caprinos
Brasil
Goats
Brazil
title_short First report of Anaplasma marginale infection in goats, Brazil
title_full First report of Anaplasma marginale infection in goats, Brazil
title_fullStr First report of Anaplasma marginale infection in goats, Brazil
title_full_unstemmed First report of Anaplasma marginale infection in goats, Brazil
title_sort First report of Anaplasma marginale infection in goats, Brazil
dc.creator.none.fl_str_mv da Silva, Nayara B.
Taus, Naomi S.
Johnson, Wendell C.
Mira, Anabela
Schnittger, Leonhard
Valente, Jessica D. M.
Vidotto, Odilon
Masterson, Hayley E.
Vieira, Thállitha S. W. J.
Ueti, Massaro W.
Vieira, Rafael F. C.
author da Silva, Nayara B.
author_facet da Silva, Nayara B.
Taus, Naomi S.
Johnson, Wendell C.
Mira, Anabela
Schnittger, Leonhard
Valente, Jessica D. M.
Vidotto, Odilon
Masterson, Hayley E.
Vieira, Thállitha S. W. J.
Ueti, Massaro W.
Vieira, Rafael F. C.
author_role author
author2 Taus, Naomi S.
Johnson, Wendell C.
Mira, Anabela
Schnittger, Leonhard
Valente, Jessica D. M.
Vidotto, Odilon
Masterson, Hayley E.
Vieira, Thállitha S. W. J.
Ueti, Massaro W.
Vieira, Rafael F. C.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anaplasma marginale
Caprinos
Brasil
Goats
Brazil
topic Anaplasma marginale
Caprinos
Brasil
Goats
Brazil
dc.description.none.fl_txt_mv Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.
Instituto de Patobiología
Fil: da Silva, Nayara B. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
Fil: Taus, Naomi S. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Johnson, Wendell C. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos
Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Valente, Jessica D. M. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
Fil: Vidotto, Odilon. Universidade Estadual de Londrina. Department of Preventive Veterinary Medicine; Brasil
Fil: Masterson, Hayley E. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Vieira, Thállitha S. W. J. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
Fil: Ueti, Massaro W. United States Department of Agriculture. Animal Diseases Research Unit; Estados Unidos. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Vieira, Rafael F. C. Universidade Federal do Parana. Department of Veterinary Medicine; Brasil
description Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-05T11:19:11Z
2018-11-05T11:19:11Z
2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202140
http://hdl.handle.net/20.500.12123/3776
1932-6203
https://doi.org/10.1371/journal.pone.0202140
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202140
http://hdl.handle.net/20.500.12123/3776
https://doi.org/10.1371/journal.pone.0202140
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv PLoS One 13 (8) : e0202140 (Agosto 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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