First report of Anaplasma marginale infection in goats, Brazil

Autores
Da Silva, Nayara B.; Taus, Naomi S.; Johnson, Wendell C.; Mira, Anabela; Schnittger, Leonhard; Valente, Jessica D. M.; Vidotto, Odilon; Masterson, Hayley E.; Vieira, Thállitha S. W. J.; Ueti, Massaro W.; Vieira, Rafael F. C.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.
Fil: Da Silva, Nayara B.. Universidade Federal do Paraná; Brasil
Fil: Taus, Naomi S.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos. Washington State University; Estados Unidos
Fil: Johnson, Wendell C.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Valente, Jessica D. M.. Universidade Federal do Paraná; Brasil
Fil: Vidotto, Odilon. Universidade Estadual de Londrina; Brasil
Fil: Masterson, Hayley E.. Washington State University; Estados Unidos
Fil: Vieira, Thállitha S. W. J.. Universidade Federal do Paraná; Brasil
Fil: Ueti, Massaro W.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos. Washington State University; Estados Unidos
Fil: Vieira, Rafael F. C.. Universidade Federal do Paraná; Brasil
Materia
ANAPLASMA MARGINALE
GOAT
PCR DETECTION
BRAZIL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117573
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/117573
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of Anaplasma marginale infection in goats, BrazilDa Silva, Nayara B.Taus, Naomi S.Johnson, Wendell C.Mira, AnabelaSchnittger, LeonhardValente, Jessica D. M.Vidotto, OdilonMasterson, Hayley E.Vieira, Thállitha S. W. J.Ueti, Massaro W.Vieira, Rafael F. C.ANAPLASMA MARGINALEGOATPCR DETECTIONBRAZILhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.Fil: Da Silva, Nayara B.. Universidade Federal do Paraná; BrasilFil: Taus, Naomi S.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos. Washington State University; Estados UnidosFil: Johnson, Wendell C.. United States Department of Agriculture. Agriculture Research Service; Estados UnidosFil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Valente, Jessica D. M.. Universidade Federal do Paraná; BrasilFil: Vidotto, Odilon. Universidade Estadual de Londrina; BrasilFil: Masterson, Hayley E.. Washington State University; Estados UnidosFil: Vieira, Thállitha S. W. J.. Universidade Federal do Paraná; BrasilFil: Ueti, Massaro W.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos. Washington State University; Estados UnidosFil: Vieira, Rafael F. C.. Universidade Federal do Paraná; BrasilPublic Library of Science2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117573Da Silva, Nayara B.; Taus, Naomi S.; Johnson, Wendell C.; Mira, Anabela; Schnittger, Leonhard; et al.; First report of Anaplasma marginale infection in goats, Brazil; Public Library of Science; Plos One; 13; 8; 8-2018; 1-61932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0202140info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202140info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:08Zoai:ri.conicet.gov.ar:11336/117573instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:08.584CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of Anaplasma marginale infection in goats, Brazil
title First report of Anaplasma marginale infection in goats, Brazil
spellingShingle First report of Anaplasma marginale infection in goats, Brazil
Da Silva, Nayara B.
ANAPLASMA MARGINALE
GOAT
PCR DETECTION
BRAZIL
title_short First report of Anaplasma marginale infection in goats, Brazil
title_full First report of Anaplasma marginale infection in goats, Brazil
title_fullStr First report of Anaplasma marginale infection in goats, Brazil
title_full_unstemmed First report of Anaplasma marginale infection in goats, Brazil
title_sort First report of Anaplasma marginale infection in goats, Brazil
dc.creator.none.fl_str_mv Da Silva, Nayara B.
Taus, Naomi S.
Johnson, Wendell C.
Mira, Anabela
Schnittger, Leonhard
Valente, Jessica D. M.
Vidotto, Odilon
Masterson, Hayley E.
Vieira, Thállitha S. W. J.
Ueti, Massaro W.
Vieira, Rafael F. C.
author Da Silva, Nayara B.
author_facet Da Silva, Nayara B.
Taus, Naomi S.
Johnson, Wendell C.
Mira, Anabela
Schnittger, Leonhard
Valente, Jessica D. M.
Vidotto, Odilon
Masterson, Hayley E.
Vieira, Thállitha S. W. J.
Ueti, Massaro W.
Vieira, Rafael F. C.
author_role author
author2 Taus, Naomi S.
Johnson, Wendell C.
Mira, Anabela
Schnittger, Leonhard
Valente, Jessica D. M.
Vidotto, Odilon
Masterson, Hayley E.
Vieira, Thállitha S. W. J.
Ueti, Massaro W.
Vieira, Rafael F. C.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANAPLASMA MARGINALE
GOAT
PCR DETECTION
BRAZIL
topic ANAPLASMA MARGINALE
GOAT
PCR DETECTION
BRAZIL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.
Fil: Da Silva, Nayara B.. Universidade Federal do Paraná; Brasil
Fil: Taus, Naomi S.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos. Washington State University; Estados Unidos
Fil: Johnson, Wendell C.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Valente, Jessica D. M.. Universidade Federal do Paraná; Brasil
Fil: Vidotto, Odilon. Universidade Estadual de Londrina; Brasil
Fil: Masterson, Hayley E.. Washington State University; Estados Unidos
Fil: Vieira, Thállitha S. W. J.. Universidade Federal do Paraná; Brasil
Fil: Ueti, Massaro W.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos. Washington State University; Estados Unidos
Fil: Vieira, Rafael F. C.. Universidade Federal do Paraná; Brasil
description Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117573
Da Silva, Nayara B.; Taus, Naomi S.; Johnson, Wendell C.; Mira, Anabela; Schnittger, Leonhard; et al.; First report of Anaplasma marginale infection in goats, Brazil; Public Library of Science; Plos One; 13; 8; 8-2018; 1-6
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117573
identifier_str_mv Da Silva, Nayara B.; Taus, Naomi S.; Johnson, Wendell C.; Mira, Anabela; Schnittger, Leonhard; et al.; First report of Anaplasma marginale infection in goats, Brazil; Public Library of Science; Plos One; 13; 8; 8-2018; 1-6
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0202140
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202140
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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