Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi

Autores
Melignani, Eliana; Guidobono, Juan Santiago; Rojo, Rodrigo; Levin, Laura; Barrera, Viviana Andrea
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ten Argentinean Waitea circinata-like isolates (Corticiales, Basidiomycota) obtained from soil samples were identified to species level based on morphological features and molecular tools, and tested in order to explore their biological control abilities. Eight of them were identified as Waitea arvalis and two as W. circinata. Waitea arvalis isolates were tested as antagonists, and W. circinata isolates were used as pathogens. Most isolates performed well in in vitro antagonistic assays, inhibiting by 54–59% the growth of seven plant pathogens: Bipolaris sorokiniana, Fusarium graminearum, Fusarium oxysporum, Pythium irregulare, two different isolates of W. circinata and Sclerotium rolfsii. The most inhibited pathogen was P. irregulare (72.6%), followed by S. rolfsii (66.7%) and W. circinata (~ 65%). There was no evidence of mycoparasitism. Their capacity for chitinase, cellulase, protease and ligninase (laccase, Mn-peroxidase and lignin-peroxidase) secretion was evaluated. Most of the isolates tested positive for chitinase activity. All the evaluated W. arvalis isolates produced cellulase, protease and laccase activities, and some of them also rendered positive results for Mn-peroxidase and decolorized the dye Azure B. Isolates INTA-L003 and INTA-L005 displayed top results for cellulase activity (both in carboxymethylcellulose plate assay and in filter paper tube assay). These activities might be responsible for their better antagonistic behavior on Pythium sp. Thus, these isolates are promising as biocontrol agents and as sources of new enzymes for industrial applications. Moreover, to the best of our knowledge, this is the first citation of W. arvalis in Argentina.
Centro de Investigación en Ciencias Veterinarias y Agronómicas
Fil: Melignani, Eliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina
Fil: Melignani, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guidobono, Juan Santiago. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Instituto de Ecología y Genética de Buenos Aires. Laboratorio de Ecología de Poblaciones; Argentina
Fil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Levin, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina
Fil: Levin, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Grupo Interacción Microorganismo-Planta; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Barrera, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Mycological Progress 23 : 16 (Published: 24 February 2024)
Materia
Laetisaria
Cellulose
Biological Control
Pythium
Basidiomycota
Plant Pathology
Celulosa
Control Biológico
Fitopatología
Waitea
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungiMelignani, ElianaGuidobono, Juan SantiagoRojo, RodrigoLevin, LauraBarrera, Viviana AndreaLaetisariaCelluloseBiological ControlPythiumBasidiomycotaPlant PathologyCelulosaControl BiológicoFitopatologíaWaiteaTen Argentinean Waitea circinata-like isolates (Corticiales, Basidiomycota) obtained from soil samples were identified to species level based on morphological features and molecular tools, and tested in order to explore their biological control abilities. Eight of them were identified as Waitea arvalis and two as W. circinata. Waitea arvalis isolates were tested as antagonists, and W. circinata isolates were used as pathogens. Most isolates performed well in in vitro antagonistic assays, inhibiting by 54–59% the growth of seven plant pathogens: Bipolaris sorokiniana, Fusarium graminearum, Fusarium oxysporum, Pythium irregulare, two different isolates of W. circinata and Sclerotium rolfsii. The most inhibited pathogen was P. irregulare (72.6%), followed by S. rolfsii (66.7%) and W. circinata (~ 65%). There was no evidence of mycoparasitism. Their capacity for chitinase, cellulase, protease and ligninase (laccase, Mn-peroxidase and lignin-peroxidase) secretion was evaluated. Most of the isolates tested positive for chitinase activity. All the evaluated W. arvalis isolates produced cellulase, protease and laccase activities, and some of them also rendered positive results for Mn-peroxidase and decolorized the dye Azure B. Isolates INTA-L003 and INTA-L005 displayed top results for cellulase activity (both in carboxymethylcellulose plate assay and in filter paper tube assay). These activities might be responsible for their better antagonistic behavior on Pythium sp. Thus, these isolates are promising as biocontrol agents and as sources of new enzymes for industrial applications. Moreover, to the best of our knowledge, this is the first citation of W. arvalis in Argentina.Centro de Investigación en Ciencias Veterinarias y AgronómicasFil: Melignani, Eliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; ArgentinaFil: Melignani, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guidobono, Juan Santiago. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Instituto de Ecología y Genética de Buenos Aires. Laboratorio de Ecología de Poblaciones; ArgentinaFil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Levin, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; ArgentinaFil: Levin, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Grupo Interacción Microorganismo-Planta; ArgentinaFil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Barrera, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2024-06-24T10:17:56Z2024-06-24T10:17:56Z2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18232https://link.springer.com/article/10.1007/s11557-024-01944-61617-416Xhttps://doi.org/10.1007/s11557-024-01944-6Mycological Progress 23 : 16 (Published: 24 February 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetalinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:36Zoai:localhost:20.500.12123/18232instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:36.974INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
title Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
spellingShingle Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
Melignani, Eliana
Laetisaria
Cellulose
Biological Control
Pythium
Basidiomycota
Plant Pathology
Celulosa
Control Biológico
Fitopatología
Waitea
title_short Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
title_full Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
title_fullStr Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
title_full_unstemmed Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
title_sort Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
dc.creator.none.fl_str_mv Melignani, Eliana
Guidobono, Juan Santiago
Rojo, Rodrigo
Levin, Laura
Barrera, Viviana Andrea
author Melignani, Eliana
author_facet Melignani, Eliana
Guidobono, Juan Santiago
Rojo, Rodrigo
Levin, Laura
Barrera, Viviana Andrea
author_role author
author2 Guidobono, Juan Santiago
Rojo, Rodrigo
Levin, Laura
Barrera, Viviana Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv Laetisaria
Cellulose
Biological Control
Pythium
Basidiomycota
Plant Pathology
Celulosa
Control Biológico
Fitopatología
Waitea
topic Laetisaria
Cellulose
Biological Control
Pythium
Basidiomycota
Plant Pathology
Celulosa
Control Biológico
Fitopatología
Waitea
dc.description.none.fl_txt_mv Ten Argentinean Waitea circinata-like isolates (Corticiales, Basidiomycota) obtained from soil samples were identified to species level based on morphological features and molecular tools, and tested in order to explore their biological control abilities. Eight of them were identified as Waitea arvalis and two as W. circinata. Waitea arvalis isolates were tested as antagonists, and W. circinata isolates were used as pathogens. Most isolates performed well in in vitro antagonistic assays, inhibiting by 54–59% the growth of seven plant pathogens: Bipolaris sorokiniana, Fusarium graminearum, Fusarium oxysporum, Pythium irregulare, two different isolates of W. circinata and Sclerotium rolfsii. The most inhibited pathogen was P. irregulare (72.6%), followed by S. rolfsii (66.7%) and W. circinata (~ 65%). There was no evidence of mycoparasitism. Their capacity for chitinase, cellulase, protease and ligninase (laccase, Mn-peroxidase and lignin-peroxidase) secretion was evaluated. Most of the isolates tested positive for chitinase activity. All the evaluated W. arvalis isolates produced cellulase, protease and laccase activities, and some of them also rendered positive results for Mn-peroxidase and decolorized the dye Azure B. Isolates INTA-L003 and INTA-L005 displayed top results for cellulase activity (both in carboxymethylcellulose plate assay and in filter paper tube assay). These activities might be responsible for their better antagonistic behavior on Pythium sp. Thus, these isolates are promising as biocontrol agents and as sources of new enzymes for industrial applications. Moreover, to the best of our knowledge, this is the first citation of W. arvalis in Argentina.
Centro de Investigación en Ciencias Veterinarias y Agronómicas
Fil: Melignani, Eliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina
Fil: Melignani, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guidobono, Juan Santiago. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Instituto de Ecología y Genética de Buenos Aires. Laboratorio de Ecología de Poblaciones; Argentina
Fil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Levin, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina
Fil: Levin, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Grupo Interacción Microorganismo-Planta; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Barrera, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Ten Argentinean Waitea circinata-like isolates (Corticiales, Basidiomycota) obtained from soil samples were identified to species level based on morphological features and molecular tools, and tested in order to explore their biological control abilities. Eight of them were identified as Waitea arvalis and two as W. circinata. Waitea arvalis isolates were tested as antagonists, and W. circinata isolates were used as pathogens. Most isolates performed well in in vitro antagonistic assays, inhibiting by 54–59% the growth of seven plant pathogens: Bipolaris sorokiniana, Fusarium graminearum, Fusarium oxysporum, Pythium irregulare, two different isolates of W. circinata and Sclerotium rolfsii. The most inhibited pathogen was P. irregulare (72.6%), followed by S. rolfsii (66.7%) and W. circinata (~ 65%). There was no evidence of mycoparasitism. Their capacity for chitinase, cellulase, protease and ligninase (laccase, Mn-peroxidase and lignin-peroxidase) secretion was evaluated. Most of the isolates tested positive for chitinase activity. All the evaluated W. arvalis isolates produced cellulase, protease and laccase activities, and some of them also rendered positive results for Mn-peroxidase and decolorized the dye Azure B. Isolates INTA-L003 and INTA-L005 displayed top results for cellulase activity (both in carboxymethylcellulose plate assay and in filter paper tube assay). These activities might be responsible for their better antagonistic behavior on Pythium sp. Thus, these isolates are promising as biocontrol agents and as sources of new enzymes for industrial applications. Moreover, to the best of our knowledge, this is the first citation of W. arvalis in Argentina.
publishDate 2024
dc.date.none.fl_str_mv 2024-06-24T10:17:56Z
2024-06-24T10:17:56Z
2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18232
https://link.springer.com/article/10.1007/s11557-024-01944-6
1617-416X
https://doi.org/10.1007/s11557-024-01944-6
url http://hdl.handle.net/20.500.12123/18232
https://link.springer.com/article/10.1007/s11557-024-01944-6
https://doi.org/10.1007/s11557-024-01944-6
identifier_str_mv 1617-416X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Mycological Progress 23 : 16 (Published: 24 February 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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