Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization

Autores
Caputo, Carla; Gomez, Federico M.; Ciolfi, Federico; Puente, Mariana Laura; Criado, María Victoria
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cereal crops require high nitrogen (N) inputs, but fertilizer use is inefficient, increasing costs and environmental risks. The use of microbial inoculants such as Azospirillum argentinense Az39 and the improvement of N remobilization are promising strategies for improving N use efficiency. We evaluated how A. argentinense Az39 modulates N economy in malting barley under different nutritional conditions, emphasizing N remobilization, post-anthesis uptake, and underlying mechanisms, as well as their combined impact on grain yield and quality. Greenhouse factorial trials combining Az39 inoculation and chemical fertilization demonstrated that Az39 modified the preference of the N source reaching the grain by increasing N remobilization relative to N uptake after anthesis, thereby improving N use efficiency and reducing dependence on soil N after anthesis. This occurred without changing the total N in the shoot, but rather its distribution, as inoculation with Az39 improved N in the leaves, particularly proteins and Rubisco. In addition, N remobilization improved with differentiated contributions from tissues to grain N, with leaves contributing mainly to net N remobilization and stems to remobilization efficiency. Inoculation also improves overall yield but mainly boosted individual grain weight without compromising grain N concentration, probably due to the increased carbohydrate export from leaves. These effects may be associated with high levels of indoleacetic acid in the leaves, which could contribute to both increased leaf N accumulation and improved grain development. Therefore, Az39 represents a valuable tool for sustainable agricultural management, enhancing N remobilization and use efficiency, as well as yield and grain quality in malting barley.
Instituto de Microbiología y Zoología Agrícola
Fil: Caputo, Carla. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Caputo, Carla. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Caputo, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Gomez, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra; Argentina.
Fil: Gomez, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Gomez, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Ciolfi, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Ciolfi, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Criado, María Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos; Argentina
Fil: Criado, María Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Criado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fuente
Discover Plants 2 : article number 342. (November 2025)
Materia
Cebada
Nitrógeno
Aplicación de Abonos
Barley
Nitrogen
Azospirillum
Fertilizer Application
Rhizobacteria
Azospirillum argentinense
Fertilización
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24789
Acceder
id INTADig_dda59a38f264fb6f428b147409b9c15c
oai_identifier_str oai:localhost:20.500.12123/24789
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilizationCaputo, CarlaGomez, Federico M.Ciolfi, FedericoPuente, Mariana LauraCriado, María VictoriaCebadaNitrógenoAplicación de AbonosBarleyNitrogenAzospirillumFertilizer ApplicationRhizobacteriaAzospirillum argentinenseFertilizaciónCereal crops require high nitrogen (N) inputs, but fertilizer use is inefficient, increasing costs and environmental risks. The use of microbial inoculants such as Azospirillum argentinense Az39 and the improvement of N remobilization are promising strategies for improving N use efficiency. We evaluated how A. argentinense Az39 modulates N economy in malting barley under different nutritional conditions, emphasizing N remobilization, post-anthesis uptake, and underlying mechanisms, as well as their combined impact on grain yield and quality. Greenhouse factorial trials combining Az39 inoculation and chemical fertilization demonstrated that Az39 modified the preference of the N source reaching the grain by increasing N remobilization relative to N uptake after anthesis, thereby improving N use efficiency and reducing dependence on soil N after anthesis. This occurred without changing the total N in the shoot, but rather its distribution, as inoculation with Az39 improved N in the leaves, particularly proteins and Rubisco. In addition, N remobilization improved with differentiated contributions from tissues to grain N, with leaves contributing mainly to net N remobilization and stems to remobilization efficiency. Inoculation also improves overall yield but mainly boosted individual grain weight without compromising grain N concentration, probably due to the increased carbohydrate export from leaves. These effects may be associated with high levels of indoleacetic acid in the leaves, which could contribute to both increased leaf N accumulation and improved grain development. Therefore, Az39 represents a valuable tool for sustainable agricultural management, enhancing N remobilization and use efficiency, as well as yield and grain quality in malting barley.Instituto de Microbiología y Zoología AgrícolaFil: Caputo, Carla. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Caputo, Carla. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Caputo, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Gomez, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra; Argentina.Fil: Gomez, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Gomez, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Ciolfi, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Ciolfi, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Criado, María Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos; ArgentinaFil: Criado, María Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Criado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaSpringer2025-12-29T11:59:48Z2025-12-29T11:59:48Z2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24789https://link.springer.com/article/10.1007/s44372-025-00427-63005-1207https://doi.org/10.1007/s44372-025-00427-6Discover Plants 2 : article number 342. (November 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:41:01Zoai:localhost:20.500.12123/24789instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:41:01.615INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
title Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
spellingShingle Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
Caputo, Carla
Cebada
Nitrógeno
Aplicación de Abonos
Barley
Nitrogen
Azospirillum
Fertilizer Application
Rhizobacteria
Azospirillum argentinense
Fertilización
title_short Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
title_full Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
title_fullStr Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
title_full_unstemmed Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
title_sort Mechanistic insights into how Azospirillum argentinense Az39 improves nitrogen economy and grain quality in barley independently of chemical fertilization
dc.creator.none.fl_str_mv Caputo, Carla
Gomez, Federico M.
Ciolfi, Federico
Puente, Mariana Laura
Criado, María Victoria
author Caputo, Carla
author_facet Caputo, Carla
Gomez, Federico M.
Ciolfi, Federico
Puente, Mariana Laura
Criado, María Victoria
author_role author
author2 Gomez, Federico M.
Ciolfi, Federico
Puente, Mariana Laura
Criado, María Victoria
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cebada
Nitrógeno
Aplicación de Abonos
Barley
Nitrogen
Azospirillum
Fertilizer Application
Rhizobacteria
Azospirillum argentinense
Fertilización
topic Cebada
Nitrógeno
Aplicación de Abonos
Barley
Nitrogen
Azospirillum
Fertilizer Application
Rhizobacteria
Azospirillum argentinense
Fertilización
dc.description.none.fl_txt_mv Cereal crops require high nitrogen (N) inputs, but fertilizer use is inefficient, increasing costs and environmental risks. The use of microbial inoculants such as Azospirillum argentinense Az39 and the improvement of N remobilization are promising strategies for improving N use efficiency. We evaluated how A. argentinense Az39 modulates N economy in malting barley under different nutritional conditions, emphasizing N remobilization, post-anthesis uptake, and underlying mechanisms, as well as their combined impact on grain yield and quality. Greenhouse factorial trials combining Az39 inoculation and chemical fertilization demonstrated that Az39 modified the preference of the N source reaching the grain by increasing N remobilization relative to N uptake after anthesis, thereby improving N use efficiency and reducing dependence on soil N after anthesis. This occurred without changing the total N in the shoot, but rather its distribution, as inoculation with Az39 improved N in the leaves, particularly proteins and Rubisco. In addition, N remobilization improved with differentiated contributions from tissues to grain N, with leaves contributing mainly to net N remobilization and stems to remobilization efficiency. Inoculation also improves overall yield but mainly boosted individual grain weight without compromising grain N concentration, probably due to the increased carbohydrate export from leaves. These effects may be associated with high levels of indoleacetic acid in the leaves, which could contribute to both increased leaf N accumulation and improved grain development. Therefore, Az39 represents a valuable tool for sustainable agricultural management, enhancing N remobilization and use efficiency, as well as yield and grain quality in malting barley.
Instituto de Microbiología y Zoología Agrícola
Fil: Caputo, Carla. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Caputo, Carla. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Caputo, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Gomez, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra; Argentina.
Fil: Gomez, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Gomez, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Ciolfi, Federico. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Ciolfi, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Criado, María Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos; Argentina
Fil: Criado, María Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Criado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
description Cereal crops require high nitrogen (N) inputs, but fertilizer use is inefficient, increasing costs and environmental risks. The use of microbial inoculants such as Azospirillum argentinense Az39 and the improvement of N remobilization are promising strategies for improving N use efficiency. We evaluated how A. argentinense Az39 modulates N economy in malting barley under different nutritional conditions, emphasizing N remobilization, post-anthesis uptake, and underlying mechanisms, as well as their combined impact on grain yield and quality. Greenhouse factorial trials combining Az39 inoculation and chemical fertilization demonstrated that Az39 modified the preference of the N source reaching the grain by increasing N remobilization relative to N uptake after anthesis, thereby improving N use efficiency and reducing dependence on soil N after anthesis. This occurred without changing the total N in the shoot, but rather its distribution, as inoculation with Az39 improved N in the leaves, particularly proteins and Rubisco. In addition, N remobilization improved with differentiated contributions from tissues to grain N, with leaves contributing mainly to net N remobilization and stems to remobilization efficiency. Inoculation also improves overall yield but mainly boosted individual grain weight without compromising grain N concentration, probably due to the increased carbohydrate export from leaves. These effects may be associated with high levels of indoleacetic acid in the leaves, which could contribute to both increased leaf N accumulation and improved grain development. Therefore, Az39 represents a valuable tool for sustainable agricultural management, enhancing N remobilization and use efficiency, as well as yield and grain quality in malting barley.
publishDate 2025
dc.date.none.fl_str_mv 2025-12-29T11:59:48Z
2025-12-29T11:59:48Z
2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24789
https://link.springer.com/article/10.1007/s44372-025-00427-6
3005-1207
https://doi.org/10.1007/s44372-025-00427-6
url http://hdl.handle.net/20.500.12123/24789
https://link.springer.com/article/10.1007/s44372-025-00427-6
https://doi.org/10.1007/s44372-025-00427-6
identifier_str_mv 3005-1207
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Discover Plants 2 : article number 342. (November 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1853758529029013504
score 13.25844

Publicaciones similares