High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana

Autores
Pereyra, Cintia; Parola, Rodrigo; Lando, Ana Paula; Rodriguez, Marianela; Martínez Noël, Giselle María Astrid
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sugars are not only energy sources, but also central molecular signals that regulate growth, development, and response to environmental stresses. It is well recognized that sugars activate TOR kinase, the key integrator of energy availability that connects external and internal signals to regulate growth in eukaryotes. However, the specific effect of different types of sugars and their concentrations on TOR signaling has not been studied in detail. Thus, we studied the activation of the root apical meristem (RAM), which is a TOR-dependent process, and TOR activity in vivo in seedlings of Arabidopsis thaliana treated with different types and concentrations of sugars. The results show that exogenous sucrose (Suc), glucose (Glc), and fructose (in a minor extent), induced RAM reactivation and TOR activity; however, at high concentration, Glc did not induce the RAM nor the kinase activity. In contrast, trehalose was not able to affect RAM length or activate TOR. High concentration of sugars, which resemble some stress conditions, inhibited TOR activity.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Pereyra, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina
Fil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Parola, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina
Fuente
Journal of Plant Growth Regulation 42 (7) : 4309–4323 (July 2023)
Materia
Auxins
Sucrose
Trehalose
Auxinas
Estres
Sucrosa
Stress
Trehalosa
Arabidopsis thaliana
TOR kinase
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16038
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16038
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thalianaPereyra, CintiaParola, RodrigoLando, Ana PaulaRodriguez, MarianelaMartínez Noël, Giselle María AstridAuxinsSucroseTrehaloseAuxinasEstresSucrosaStressTrehalosaArabidopsis thalianaTOR kinaseSugars are not only energy sources, but also central molecular signals that regulate growth, development, and response to environmental stresses. It is well recognized that sugars activate TOR kinase, the key integrator of energy availability that connects external and internal signals to regulate growth in eukaryotes. However, the specific effect of different types of sugars and their concentrations on TOR signaling has not been studied in detail. Thus, we studied the activation of the root apical meristem (RAM), which is a TOR-dependent process, and TOR activity in vivo in seedlings of Arabidopsis thaliana treated with different types and concentrations of sugars. The results show that exogenous sucrose (Suc), glucose (Glc), and fructose (in a minor extent), induced RAM reactivation and TOR activity; however, at high concentration, Glc did not induce the RAM nor the kinase activity. In contrast, trehalose was not able to affect RAM length or activate TOR. High concentration of sugars, which resemble some stress conditions, inhibited TOR activity.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Pereyra, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); ArgentinaFil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Parola, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaFil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); ArgentinaFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaFil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); ArgentinaSpringer2023-11-28T12:12:44Z2023-11-28T12:12:44Z2023-01-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16038https://link.springer.com/article/10.1007/s00344-022-10894-w0721-75951435-8107 (online)https://doi.org/10.1007/s00344-022-10894-wJournal of Plant Growth Regulation 42 (7) : 4309–4323 (July 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrialinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:05Zoai:localhost:20.500.12123/16038instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:06.162INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
title High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
spellingShingle High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
Pereyra, Cintia
Auxins
Sucrose
Trehalose
Auxinas
Estres
Sucrosa
Stress
Trehalosa
Arabidopsis thaliana
TOR kinase
title_short High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
title_full High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
title_fullStr High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
title_full_unstemmed High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
title_sort High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
dc.creator.none.fl_str_mv Pereyra, Cintia
Parola, Rodrigo
Lando, Ana Paula
Rodriguez, Marianela
Martínez Noël, Giselle María Astrid
author Pereyra, Cintia
author_facet Pereyra, Cintia
Parola, Rodrigo
Lando, Ana Paula
Rodriguez, Marianela
Martínez Noël, Giselle María Astrid
author_role author
author2 Parola, Rodrigo
Lando, Ana Paula
Rodriguez, Marianela
Martínez Noël, Giselle María Astrid
author2_role author
author
author
author
dc.subject.none.fl_str_mv Auxins
Sucrose
Trehalose
Auxinas
Estres
Sucrosa
Stress
Trehalosa
Arabidopsis thaliana
TOR kinase
topic Auxins
Sucrose
Trehalose
Auxinas
Estres
Sucrosa
Stress
Trehalosa
Arabidopsis thaliana
TOR kinase
dc.description.none.fl_txt_mv Sugars are not only energy sources, but also central molecular signals that regulate growth, development, and response to environmental stresses. It is well recognized that sugars activate TOR kinase, the key integrator of energy availability that connects external and internal signals to regulate growth in eukaryotes. However, the specific effect of different types of sugars and their concentrations on TOR signaling has not been studied in detail. Thus, we studied the activation of the root apical meristem (RAM), which is a TOR-dependent process, and TOR activity in vivo in seedlings of Arabidopsis thaliana treated with different types and concentrations of sugars. The results show that exogenous sucrose (Suc), glucose (Glc), and fructose (in a minor extent), induced RAM reactivation and TOR activity; however, at high concentration, Glc did not induce the RAM nor the kinase activity. In contrast, trehalose was not able to affect RAM length or activate TOR. High concentration of sugars, which resemble some stress conditions, inhibited TOR activity.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Pereyra, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina
Fil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Parola, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina
description Sugars are not only energy sources, but also central molecular signals that regulate growth, development, and response to environmental stresses. It is well recognized that sugars activate TOR kinase, the key integrator of energy availability that connects external and internal signals to regulate growth in eukaryotes. However, the specific effect of different types of sugars and their concentrations on TOR signaling has not been studied in detail. Thus, we studied the activation of the root apical meristem (RAM), which is a TOR-dependent process, and TOR activity in vivo in seedlings of Arabidopsis thaliana treated with different types and concentrations of sugars. The results show that exogenous sucrose (Suc), glucose (Glc), and fructose (in a minor extent), induced RAM reactivation and TOR activity; however, at high concentration, Glc did not induce the RAM nor the kinase activity. In contrast, trehalose was not able to affect RAM length or activate TOR. High concentration of sugars, which resemble some stress conditions, inhibited TOR activity.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-28T12:12:44Z
2023-11-28T12:12:44Z
2023-01-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16038
https://link.springer.com/article/10.1007/s00344-022-10894-w
0721-7595
1435-8107 (online)
https://doi.org/10.1007/s00344-022-10894-w
url http://hdl.handle.net/20.500.12123/16038
https://link.springer.com/article/10.1007/s00344-022-10894-w
https://doi.org/10.1007/s00344-022-10894-w
identifier_str_mv 0721-7595
1435-8107 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Plant Growth Regulation 42 (7) : 4309–4323 (July 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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