Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana

Autores
Lando, Ana Paula; Terrile, María Cecilia; De Marco, María Agustina; Rodriguez, Marianela; Martínez Noël, Giselle María Astrid
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitric oxide (NO) constitutes a signal molecule that regulates important target proteins related to growth and development and also contributes to metabolic reprogramming that occurs under adverse conditions. Taking into account the important role of NO and its relationship with Target of Rapamycin (TOR) signaling in animals, we wondered about the putative link between both pathways in plants. With this aim, we studied a TOR-dependent process which is the reactivation of the root apical meristem (RAM) in Arabidopsis thaliana. We used pharmacological and genetic tools to evaluate the relationship between NO and TOR on the sugar induction of RAM, using SNP as NO donor, cPTIO as NO scavenger and the nitrate reductase (NR) mutant nia2. The results showed that sucrose (SUC)-mediated TOR activation of the RAM requires NO and that NO, in turn, participates in the regulation of TOR signaling. Interestingly, TOR activation induced by sugar increased the NO levels. We also observed that NO could mediate the repression of SnRK1 activity by SUC. By computational prediction we found putative S-nitrosylation sites in the TOR complex proteins and the catalytic subunit of SnRK1, SnRK1.1. The present work demonstrates for the first time a link between NO and TOR revealing the complex interplay between the two pathways in plants.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); Argentina
Fil: Terrile, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas; Argentina
Fil: Terrile, María Cecilia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; Argentina
Fil: De Marco, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); Argentina
Fuente
Planta 260 : article number 113. (October 2024)
Materia
Sucrosa
Óxido Nítrico
Meristema
Sucrose
Nitric Oxide
Arabidopsis thaliana
Meristems
TOR kinase
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22252
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22252
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thalianaLando, Ana PaulaTerrile, María CeciliaDe Marco, María AgustinaRodriguez, MarianelaMartínez Noël, Giselle María AstridSucrosaÓxido NítricoMeristemaSucroseNitric OxideArabidopsis thalianaMeristemsTOR kinaseNitric oxide (NO) constitutes a signal molecule that regulates important target proteins related to growth and development and also contributes to metabolic reprogramming that occurs under adverse conditions. Taking into account the important role of NO and its relationship with Target of Rapamycin (TOR) signaling in animals, we wondered about the putative link between both pathways in plants. With this aim, we studied a TOR-dependent process which is the reactivation of the root apical meristem (RAM) in Arabidopsis thaliana. We used pharmacological and genetic tools to evaluate the relationship between NO and TOR on the sugar induction of RAM, using SNP as NO donor, cPTIO as NO scavenger and the nitrate reductase (NR) mutant nia2. The results showed that sucrose (SUC)-mediated TOR activation of the RAM requires NO and that NO, in turn, participates in the regulation of TOR signaling. Interestingly, TOR activation induced by sugar increased the NO levels. We also observed that NO could mediate the repression of SnRK1 activity by SUC. By computational prediction we found putative S-nitrosylation sites in the TOR complex proteins and the catalytic subunit of SnRK1, SnRK1.1. The present work demonstrates for the first time a link between NO and TOR revealing the complex interplay between the two pathways in plants.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); ArgentinaFil: Terrile, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas; ArgentinaFil: Terrile, María Cecilia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; ArgentinaFil: De Marco, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); ArgentinaFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); ArgentinaSpringer2025-05-13T13:40:34Z2025-05-13T13:40:34Z2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22252https://link.springer.com/article/10.1007/s00425-024-04542-80032-09351432-2048https://doi.org/10.1007/s00425-024-04542-8Planta 260 : article number 113. (October 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:03Zoai:localhost:20.500.12123/22252instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:03.957INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
title Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
spellingShingle Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
Lando, Ana Paula
Sucrosa
Óxido Nítrico
Meristema
Sucrose
Nitric Oxide
Arabidopsis thaliana
Meristems
TOR kinase
title_short Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
title_full Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
title_fullStr Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
title_full_unstemmed Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
title_sort Nitric oxide participates in sucrose–TOR signaling during meristem activation in Arabidopsis thaliana
dc.creator.none.fl_str_mv Lando, Ana Paula
Terrile, María Cecilia
De Marco, María Agustina
Rodriguez, Marianela
Martínez Noël, Giselle María Astrid
author Lando, Ana Paula
author_facet Lando, Ana Paula
Terrile, María Cecilia
De Marco, María Agustina
Rodriguez, Marianela
Martínez Noël, Giselle María Astrid
author_role author
author2 Terrile, María Cecilia
De Marco, María Agustina
Rodriguez, Marianela
Martínez Noël, Giselle María Astrid
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sucrosa
Óxido Nítrico
Meristema
Sucrose
Nitric Oxide
Arabidopsis thaliana
Meristems
TOR kinase
topic Sucrosa
Óxido Nítrico
Meristema
Sucrose
Nitric Oxide
Arabidopsis thaliana
Meristems
TOR kinase
dc.description.none.fl_txt_mv Nitric oxide (NO) constitutes a signal molecule that regulates important target proteins related to growth and development and also contributes to metabolic reprogramming that occurs under adverse conditions. Taking into account the important role of NO and its relationship with Target of Rapamycin (TOR) signaling in animals, we wondered about the putative link between both pathways in plants. With this aim, we studied a TOR-dependent process which is the reactivation of the root apical meristem (RAM) in Arabidopsis thaliana. We used pharmacological and genetic tools to evaluate the relationship between NO and TOR on the sugar induction of RAM, using SNP as NO donor, cPTIO as NO scavenger and the nitrate reductase (NR) mutant nia2. The results showed that sucrose (SUC)-mediated TOR activation of the RAM requires NO and that NO, in turn, participates in the regulation of TOR signaling. Interestingly, TOR activation induced by sugar increased the NO levels. We also observed that NO could mediate the repression of SnRK1 activity by SUC. By computational prediction we found putative S-nitrosylation sites in the TOR complex proteins and the catalytic subunit of SnRK1, SnRK1.1. The present work demonstrates for the first time a link between NO and TOR revealing the complex interplay between the two pathways in plants.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); Argentina
Fil: Terrile, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas; Argentina
Fil: Terrile, María Cecilia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; Argentina
Fil: De Marco, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biológicas Aplicada (FIBA); Argentina
description Nitric oxide (NO) constitutes a signal molecule that regulates important target proteins related to growth and development and also contributes to metabolic reprogramming that occurs under adverse conditions. Taking into account the important role of NO and its relationship with Target of Rapamycin (TOR) signaling in animals, we wondered about the putative link between both pathways in plants. With this aim, we studied a TOR-dependent process which is the reactivation of the root apical meristem (RAM) in Arabidopsis thaliana. We used pharmacological and genetic tools to evaluate the relationship between NO and TOR on the sugar induction of RAM, using SNP as NO donor, cPTIO as NO scavenger and the nitrate reductase (NR) mutant nia2. The results showed that sucrose (SUC)-mediated TOR activation of the RAM requires NO and that NO, in turn, participates in the regulation of TOR signaling. Interestingly, TOR activation induced by sugar increased the NO levels. We also observed that NO could mediate the repression of SnRK1 activity by SUC. By computational prediction we found putative S-nitrosylation sites in the TOR complex proteins and the catalytic subunit of SnRK1, SnRK1.1. The present work demonstrates for the first time a link between NO and TOR revealing the complex interplay between the two pathways in plants.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
2025-05-13T13:40:34Z
2025-05-13T13:40:34Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22252
https://link.springer.com/article/10.1007/s00425-024-04542-8
0032-0935
1432-2048
https://doi.org/10.1007/s00425-024-04542-8
url http://hdl.handle.net/20.500.12123/22252
https://link.springer.com/article/10.1007/s00425-024-04542-8
https://doi.org/10.1007/s00425-024-04542-8
identifier_str_mv 0032-0935
1432-2048
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Planta 260 : article number 113. (October 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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