Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability

Autores
Figueroa, Carlos Maria; Feil, Regina; Ishihara, Hirofumi; Watanabe, Mutsumi; Kölling, Katharina; Krause, Ursula; Höhne, Melanie; Encke, Beatrice; Plaxton, William C.; Zeeman, Samuel C.; Li, Zhi; Schulze, Waltraud X.; Hoefgen, Rainer; Stitt, Mark; Lunn, John E.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trehalose 6-phosphate (Tre6P) is an essential signal metabolite in plants, linking growth and development to carbon metabolism. The sucrose-Tre6P nexus model postulates that Tre6P acts as both a signal and negative feedback regulator of sucrose levels. To test this model, short-term metabolic responses to induced increases in Tre6P levels were investigated in Arabidopsis thaliana plants expressing the Escherichia coli Tre6P synthase (otsA) under the control of an ethanol-inducible promoter. Increased Tre6P led to a transient drop in sucrose content, post-translational activation of nitrate reductase and phosphoenolpyruvate carboxylase, and increased levels of organic and amino acids. Radio- (14CO2) and stable-isotope (13CO2) labelling experiments showed no change in the rates of photoassimilate export in plants with elevated Tre6P, but increased flux of carbon into organic acids. It is concluded that high Tre6P lowers sucrose levels by stimulating nitrate assimilation and anaplerotic synthesis of organic acids, thereby diverting photoassimilates away from sucrose to generate carbon skeletons and fixed nitrogen for amino acid synthesis. These results are consistent with the sucrose-Tre6P nexus model and implicate Tre6P in coordinating carbon and nitrogen metabolism in plants.
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Watanabe, Mutsumi. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Kölling, Katharina. Institute of Agricultural Sciences; Suiza
Fil: Krause, Ursula. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Höhne, Melanie. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Encke, Beatrice. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Plaxton, William C.. Queens University; Canadá
Fil: Zeeman, Samuel C.. Institute of Agricultural Sciences; Suiza
Fil: Li, Zhi. Universidad de Hohenheim; Alemania
Fil: Schulze, Waltraud X.. Universidad de Hohenheim; Alemania
Fil: Hoefgen, Rainer. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Lunn, John E.. Max Planck Institute of Molecular Plant Physiology; Alemania
Materia
Arabidopsis Thaliana
Nitrate Reductase
Protein Phosphorylation
Phosphoenolpyruvate Carboxylase
Sucrose
Trehalose-6-Phosphate Synthase
Ubiquitination
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45420
Acceder
id CONICETDig_843e7eb79588ac0a1a526733dc9d3953
oai_identifier_str oai:ri.conicet.gov.ar:11336/45420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availabilityFigueroa, Carlos MariaFeil, ReginaIshihara, HirofumiWatanabe, MutsumiKölling, KatharinaKrause, UrsulaHöhne, MelanieEncke, BeatricePlaxton, William C.Zeeman, Samuel C.Li, ZhiSchulze, Waltraud X.Hoefgen, RainerStitt, MarkLunn, John E.Arabidopsis ThalianaNitrate ReductaseProtein PhosphorylationPhosphoenolpyruvate CarboxylaseSucroseTrehalose-6-Phosphate SynthaseUbiquitinationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trehalose 6-phosphate (Tre6P) is an essential signal metabolite in plants, linking growth and development to carbon metabolism. The sucrose-Tre6P nexus model postulates that Tre6P acts as both a signal and negative feedback regulator of sucrose levels. To test this model, short-term metabolic responses to induced increases in Tre6P levels were investigated in Arabidopsis thaliana plants expressing the Escherichia coli Tre6P synthase (otsA) under the control of an ethanol-inducible promoter. Increased Tre6P led to a transient drop in sucrose content, post-translational activation of nitrate reductase and phosphoenolpyruvate carboxylase, and increased levels of organic and amino acids. Radio- (14CO2) and stable-isotope (13CO2) labelling experiments showed no change in the rates of photoassimilate export in plants with elevated Tre6P, but increased flux of carbon into organic acids. It is concluded that high Tre6P lowers sucrose levels by stimulating nitrate assimilation and anaplerotic synthesis of organic acids, thereby diverting photoassimilates away from sucrose to generate carbon skeletons and fixed nitrogen for amino acid synthesis. These results are consistent with the sucrose-Tre6P nexus model and implicate Tre6P in coordinating carbon and nitrogen metabolism in plants.Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Ishihara, Hirofumi. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Watanabe, Mutsumi. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Kölling, Katharina. Institute of Agricultural Sciences; SuizaFil: Krause, Ursula. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Höhne, Melanie. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Encke, Beatrice. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Plaxton, William C.. Queens University; CanadáFil: Zeeman, Samuel C.. Institute of Agricultural Sciences; SuizaFil: Li, Zhi. Universidad de Hohenheim; AlemaniaFil: Schulze, Waltraud X.. Universidad de Hohenheim; AlemaniaFil: Hoefgen, Rainer. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Lunn, John E.. Max Planck Institute of Molecular Plant Physiology; AlemaniaWiley Blackwell Publishing, Inc2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45420Figueroa, Carlos Maria; Feil, Regina; Ishihara, Hirofumi; Watanabe, Mutsumi; Kölling, Katharina; et al.; Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability; Wiley Blackwell Publishing, Inc; Plant Journal; 85; 3; 2-2016; 410-4230960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.13114info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13114info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:13Zoai:ri.conicet.gov.ar:11336/45420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:13.957CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
title Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
spellingShingle Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
Figueroa, Carlos Maria
Arabidopsis Thaliana
Nitrate Reductase
Protein Phosphorylation
Phosphoenolpyruvate Carboxylase
Sucrose
Trehalose-6-Phosphate Synthase
Ubiquitination
title_short Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
title_full Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
title_fullStr Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
title_full_unstemmed Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
title_sort Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability
dc.creator.none.fl_str_mv Figueroa, Carlos Maria
Feil, Regina
Ishihara, Hirofumi
Watanabe, Mutsumi
Kölling, Katharina
Krause, Ursula
Höhne, Melanie
Encke, Beatrice
Plaxton, William C.
Zeeman, Samuel C.
Li, Zhi
Schulze, Waltraud X.
Hoefgen, Rainer
Stitt, Mark
Lunn, John E.
author Figueroa, Carlos Maria
author_facet Figueroa, Carlos Maria
Feil, Regina
Ishihara, Hirofumi
Watanabe, Mutsumi
Kölling, Katharina
Krause, Ursula
Höhne, Melanie
Encke, Beatrice
Plaxton, William C.
Zeeman, Samuel C.
Li, Zhi
Schulze, Waltraud X.
Hoefgen, Rainer
Stitt, Mark
Lunn, John E.
author_role author
author2 Feil, Regina
Ishihara, Hirofumi
Watanabe, Mutsumi
Kölling, Katharina
Krause, Ursula
Höhne, Melanie
Encke, Beatrice
Plaxton, William C.
Zeeman, Samuel C.
Li, Zhi
Schulze, Waltraud X.
Hoefgen, Rainer
Stitt, Mark
Lunn, John E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Nitrate Reductase
Protein Phosphorylation
Phosphoenolpyruvate Carboxylase
Sucrose
Trehalose-6-Phosphate Synthase
Ubiquitination
topic Arabidopsis Thaliana
Nitrate Reductase
Protein Phosphorylation
Phosphoenolpyruvate Carboxylase
Sucrose
Trehalose-6-Phosphate Synthase
Ubiquitination
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trehalose 6-phosphate (Tre6P) is an essential signal metabolite in plants, linking growth and development to carbon metabolism. The sucrose-Tre6P nexus model postulates that Tre6P acts as both a signal and negative feedback regulator of sucrose levels. To test this model, short-term metabolic responses to induced increases in Tre6P levels were investigated in Arabidopsis thaliana plants expressing the Escherichia coli Tre6P synthase (otsA) under the control of an ethanol-inducible promoter. Increased Tre6P led to a transient drop in sucrose content, post-translational activation of nitrate reductase and phosphoenolpyruvate carboxylase, and increased levels of organic and amino acids. Radio- (14CO2) and stable-isotope (13CO2) labelling experiments showed no change in the rates of photoassimilate export in plants with elevated Tre6P, but increased flux of carbon into organic acids. It is concluded that high Tre6P lowers sucrose levels by stimulating nitrate assimilation and anaplerotic synthesis of organic acids, thereby diverting photoassimilates away from sucrose to generate carbon skeletons and fixed nitrogen for amino acid synthesis. These results are consistent with the sucrose-Tre6P nexus model and implicate Tre6P in coordinating carbon and nitrogen metabolism in plants.
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Watanabe, Mutsumi. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Kölling, Katharina. Institute of Agricultural Sciences; Suiza
Fil: Krause, Ursula. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Höhne, Melanie. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Encke, Beatrice. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Plaxton, William C.. Queens University; Canadá
Fil: Zeeman, Samuel C.. Institute of Agricultural Sciences; Suiza
Fil: Li, Zhi. Universidad de Hohenheim; Alemania
Fil: Schulze, Waltraud X.. Universidad de Hohenheim; Alemania
Fil: Hoefgen, Rainer. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Lunn, John E.. Max Planck Institute of Molecular Plant Physiology; Alemania
description Trehalose 6-phosphate (Tre6P) is an essential signal metabolite in plants, linking growth and development to carbon metabolism. The sucrose-Tre6P nexus model postulates that Tre6P acts as both a signal and negative feedback regulator of sucrose levels. To test this model, short-term metabolic responses to induced increases in Tre6P levels were investigated in Arabidopsis thaliana plants expressing the Escherichia coli Tre6P synthase (otsA) under the control of an ethanol-inducible promoter. Increased Tre6P led to a transient drop in sucrose content, post-translational activation of nitrate reductase and phosphoenolpyruvate carboxylase, and increased levels of organic and amino acids. Radio- (14CO2) and stable-isotope (13CO2) labelling experiments showed no change in the rates of photoassimilate export in plants with elevated Tre6P, but increased flux of carbon into organic acids. It is concluded that high Tre6P lowers sucrose levels by stimulating nitrate assimilation and anaplerotic synthesis of organic acids, thereby diverting photoassimilates away from sucrose to generate carbon skeletons and fixed nitrogen for amino acid synthesis. These results are consistent with the sucrose-Tre6P nexus model and implicate Tre6P in coordinating carbon and nitrogen metabolism in plants.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45420
Figueroa, Carlos Maria; Feil, Regina; Ishihara, Hirofumi; Watanabe, Mutsumi; Kölling, Katharina; et al.; Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability; Wiley Blackwell Publishing, Inc; Plant Journal; 85; 3; 2-2016; 410-423
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45420
identifier_str_mv Figueroa, Carlos Maria; Feil, Regina; Ishihara, Hirofumi; Watanabe, Mutsumi; Kölling, Katharina; et al.; Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability; Wiley Blackwell Publishing, Inc; Plant Journal; 85; 3; 2-2016; 410-423
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.13114
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269081372196864
score 13.13397

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