Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)

Autores
Sebastian, Patrick; Król, Nina; Novoa, María Belén; Nijhof, Ard Menzo; Pfeffer, Martin; Nava, Santiago; Obiegala, Anna
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks (̲ = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs (̲ = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); ̲ = 45 days; SD ± 2 in the rabbit and (p = 0.0144). ̲ = 48 days; SD ± 2 in the calf). Egg cluster hatching (̲ = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: ̲ = 74%; SD ± 20; p = 0.0529; calf: ̲ = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.
EEA Rafaela
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nijhof, Ard Menzo. Freie Universität Berlin. Institute of Parasitology and Tropical Veterinary Medicine; Alemania
Fil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fuente
Microorganisms 11 (5) : 1107. (May 2023)
Materia
Amblyomma
Ticks
Feeding Systems
Tick-borne Diseases
Laboratory Animals
Laboratory Experimentation
Garrapata
Sistema de Alimentación
Enfermedad Transmitida por Garrapatas
Animal de Laboratorio
Experimentación en Laboratorio
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22258
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oai_identifier_str oai:localhost:20.500.12123/22258
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network_name_str INTA Digital (INTA)
spelling Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)Sebastian, PatrickKról, NinaNovoa, María BelénNijhof, Ard MenzoPfeffer, MartinNava, SantiagoObiegala, AnnaAmblyommaTicksFeeding SystemsTick-borne DiseasesLaboratory AnimalsLaboratory ExperimentationGarrapataSistema de AlimentaciónEnfermedad Transmitida por GarrapatasAnimal de LaboratorioExperimentación en LaboratorioHard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks (̲ = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs (̲ = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); ̲ = 45 days; SD ± 2 in the rabbit and (p = 0.0144). ̲ = 48 days; SD ± 2 in the calf). Egg cluster hatching (̲ = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: ̲ = 74%; SD ± 20; p = 0.0529; calf: ̲ = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.EEA RafaelaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaFil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nijhof, Ard Menzo. Freie Universität Berlin. Institute of Parasitology and Tropical Veterinary Medicine; AlemaniaFil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaMDPI2025-05-13T15:04:11Z2025-05-13T15:04:11Z2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22258https://www.mdpi.com/2076-2607/11/5/11072076-2607https://doi.org/10.3390/microorganisms11051107Microorganisms 11 (5) : 1107. (May 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y lainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:16Zoai:localhost:20.500.12123/22258instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:17.267INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
spellingShingle Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
Sebastian, Patrick
Amblyomma
Ticks
Feeding Systems
Tick-borne Diseases
Laboratory Animals
Laboratory Experimentation
Garrapata
Sistema de Alimentación
Enfermedad Transmitida por Garrapatas
Animal de Laboratorio
Experimentación en Laboratorio
title_short Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_full Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_fullStr Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_full_unstemmed Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_sort Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
dc.creator.none.fl_str_mv Sebastian, Patrick
Król, Nina
Novoa, María Belén
Nijhof, Ard Menzo
Pfeffer, Martin
Nava, Santiago
Obiegala, Anna
author Sebastian, Patrick
author_facet Sebastian, Patrick
Król, Nina
Novoa, María Belén
Nijhof, Ard Menzo
Pfeffer, Martin
Nava, Santiago
Obiegala, Anna
author_role author
author2 Król, Nina
Novoa, María Belén
Nijhof, Ard Menzo
Pfeffer, Martin
Nava, Santiago
Obiegala, Anna
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Amblyomma
Ticks
Feeding Systems
Tick-borne Diseases
Laboratory Animals
Laboratory Experimentation
Garrapata
Sistema de Alimentación
Enfermedad Transmitida por Garrapatas
Animal de Laboratorio
Experimentación en Laboratorio
topic Amblyomma
Ticks
Feeding Systems
Tick-borne Diseases
Laboratory Animals
Laboratory Experimentation
Garrapata
Sistema de Alimentación
Enfermedad Transmitida por Garrapatas
Animal de Laboratorio
Experimentación en Laboratorio
dc.description.none.fl_txt_mv Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks (̲ = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs (̲ = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); ̲ = 45 days; SD ± 2 in the rabbit and (p = 0.0144). ̲ = 48 days; SD ± 2 in the calf). Egg cluster hatching (̲ = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: ̲ = 74%; SD ± 20; p = 0.0529; calf: ̲ = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.
EEA Rafaela
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nijhof, Ard Menzo. Freie Universität Berlin. Institute of Parasitology and Tropical Veterinary Medicine; Alemania
Fil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
description Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks (̲ = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs (̲ = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); ̲ = 45 days; SD ± 2 in the rabbit and (p = 0.0144). ̲ = 48 days; SD ± 2 in the calf). Egg cluster hatching (̲ = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: ̲ = 74%; SD ± 20; p = 0.0529; calf: ̲ = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
2025-05-13T15:04:11Z
2025-05-13T15:04:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22258
https://www.mdpi.com/2076-2607/11/5/1107
2076-2607
https://doi.org/10.3390/microorganisms11051107
url http://hdl.handle.net/20.500.12123/22258
https://www.mdpi.com/2076-2607/11/5/1107
https://doi.org/10.3390/microorganisms11051107
identifier_str_mv 2076-2607
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Microorganisms 11 (5) : 1107. (May 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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