Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina

Autores
Tarragona, Evelina Luisa; Flores, Fernando Sebastián; Lamattina, Daniela; Torrents, Jorgelina; Sebastian, Patrick; Nava, Santiago
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The aim of this study is to determine if there is circulation of microorganisms of the genus Ehrlichia in opossums Didelphis albiventris and their ticks from the Humid Chaco in Argentina. Blood samples of 15 specimens of the opossum D. albiventris were analysed. Immature stages of the ticks Amblyomma ovale (Larvae=26; Nymphs=10), Amblyomma sculptum (Larvae=86; Nymphs=6) and Ornithodoros sp. cf. O. mimon (Larvae=90) were also analyzed. DNA was extracted individually from blood samples and ticks. Molecular detection of Ehrlichia agents was performed targeting two different loci: 16S rRNA and dsb gen. The phylogenetic analyses showed that the Ehrlichia sp. detected in D. albiventris in this study is identical to Ehrlichia sp. strain Natal previously detected in two marsupials from Brazil. Furthermore, a new Ehrlichia strain was amplified from an A. ovale nymph (named as Ehrlichia sp. strain El Bagual) which is phylogenetically closely related to a strain of Ehrlichia sp. detected in Bradypus tridactylus in Brazil. The findings of the current study represent the first report of these two strains of Ehrlichia for Argentina, showing that the diversity of Ehrlichia spp. is greater than previously assumed. Further studies should determine the epidemiological relevance of these findings.
EEA Rafaela
Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral). Laboratorio de Ecología de Enfermedades; Argentina.
Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lamattina, Daniela. Instituto Nacional de Medicina Tropical (Misiones); Argentina
Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Ticks and Tick-borne Diseases : 102043 (November 2022)
Materia
Ehrlichia
Amblyomma
Ixodidae
Tick-borne diseases
Argentina
Enfermedades Transmitidas por Garrapatas
Amblyomma ovale
Didelphis albiventris
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12919
Acceder
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oai_identifier_str oai:localhost:20.500.12123/12919
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in ArgentinaTarragona, Evelina LuisaFlores, Fernando SebastiánLamattina, DanielaTorrents, JorgelinaSebastian, PatrickNava, SantiagoEhrlichiaAmblyommaIxodidaeTick-borne diseasesArgentinaEnfermedades Transmitidas por GarrapatasAmblyomma ovaleDidelphis albiventrisThe aim of this study is to determine if there is circulation of microorganisms of the genus Ehrlichia in opossums Didelphis albiventris and their ticks from the Humid Chaco in Argentina. Blood samples of 15 specimens of the opossum D. albiventris were analysed. Immature stages of the ticks Amblyomma ovale (Larvae=26; Nymphs=10), Amblyomma sculptum (Larvae=86; Nymphs=6) and Ornithodoros sp. cf. O. mimon (Larvae=90) were also analyzed. DNA was extracted individually from blood samples and ticks. Molecular detection of Ehrlichia agents was performed targeting two different loci: 16S rRNA and dsb gen. The phylogenetic analyses showed that the Ehrlichia sp. detected in D. albiventris in this study is identical to Ehrlichia sp. strain Natal previously detected in two marsupials from Brazil. Furthermore, a new Ehrlichia strain was amplified from an A. ovale nymph (named as Ehrlichia sp. strain El Bagual) which is phylogenetically closely related to a strain of Ehrlichia sp. detected in Bradypus tridactylus in Brazil. The findings of the current study represent the first report of these two strains of Ehrlichia for Argentina, showing that the diversity of Ehrlichia spp. is greater than previously assumed. Further studies should determine the epidemiological relevance of these findings.EEA RafaelaFil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Flores, Fernando Sebastián. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral). Laboratorio de Ecología de Enfermedades; Argentina.Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lamattina, Daniela. Instituto Nacional de Medicina Tropical (Misiones); ArgentinaFil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2023-09-212022-09-21T11:13:57Z2022-09-21T11:13:57Z2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12919https://www.sciencedirect.com/science/article/pii/S1877959X22001455Evelina L. Tarragona, Fernando S. Flores, Daniela Lamattina, Jorgelina Torrents, Patrick S. Sebastian, Santiago Nava, Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina, Ticks and Tick-borne Diseases, 2022, 102043, ISSN 1877-959X, https://doi.org/10.1016/j.ttbdis.2022.102043.1877-959Xhttps://doi.org/10.1016/j.ttbdis.2022.102043Ticks and Tick-borne Diseases : 102043 (November 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y lainfo:eu-repo/semantics/openAccess2025-09-04T09:49:19Zoai:localhost:20.500.12123/12919instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:19.606INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
title Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
spellingShingle Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
Tarragona, Evelina Luisa
Ehrlichia
Amblyomma
Ixodidae
Tick-borne diseases
Argentina
Enfermedades Transmitidas por Garrapatas
Amblyomma ovale
Didelphis albiventris
title_short Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
title_full Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
title_fullStr Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
title_full_unstemmed Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
title_sort Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
dc.creator.none.fl_str_mv Tarragona, Evelina Luisa
Flores, Fernando Sebastián
Lamattina, Daniela
Torrents, Jorgelina
Sebastian, Patrick
Nava, Santiago
author Tarragona, Evelina Luisa
author_facet Tarragona, Evelina Luisa
Flores, Fernando Sebastián
Lamattina, Daniela
Torrents, Jorgelina
Sebastian, Patrick
Nava, Santiago
author_role author
author2 Flores, Fernando Sebastián
Lamattina, Daniela
Torrents, Jorgelina
Sebastian, Patrick
Nava, Santiago
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ehrlichia
Amblyomma
Ixodidae
Tick-borne diseases
Argentina
Enfermedades Transmitidas por Garrapatas
Amblyomma ovale
Didelphis albiventris
topic Ehrlichia
Amblyomma
Ixodidae
Tick-borne diseases
Argentina
Enfermedades Transmitidas por Garrapatas
Amblyomma ovale
Didelphis albiventris
dc.description.none.fl_txt_mv The aim of this study is to determine if there is circulation of microorganisms of the genus Ehrlichia in opossums Didelphis albiventris and their ticks from the Humid Chaco in Argentina. Blood samples of 15 specimens of the opossum D. albiventris were analysed. Immature stages of the ticks Amblyomma ovale (Larvae=26; Nymphs=10), Amblyomma sculptum (Larvae=86; Nymphs=6) and Ornithodoros sp. cf. O. mimon (Larvae=90) were also analyzed. DNA was extracted individually from blood samples and ticks. Molecular detection of Ehrlichia agents was performed targeting two different loci: 16S rRNA and dsb gen. The phylogenetic analyses showed that the Ehrlichia sp. detected in D. albiventris in this study is identical to Ehrlichia sp. strain Natal previously detected in two marsupials from Brazil. Furthermore, a new Ehrlichia strain was amplified from an A. ovale nymph (named as Ehrlichia sp. strain El Bagual) which is phylogenetically closely related to a strain of Ehrlichia sp. detected in Bradypus tridactylus in Brazil. The findings of the current study represent the first report of these two strains of Ehrlichia for Argentina, showing that the diversity of Ehrlichia spp. is greater than previously assumed. Further studies should determine the epidemiological relevance of these findings.
EEA Rafaela
Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral). Laboratorio de Ecología de Enfermedades; Argentina.
Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lamattina, Daniela. Instituto Nacional de Medicina Tropical (Misiones); Argentina
Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description The aim of this study is to determine if there is circulation of microorganisms of the genus Ehrlichia in opossums Didelphis albiventris and their ticks from the Humid Chaco in Argentina. Blood samples of 15 specimens of the opossum D. albiventris were analysed. Immature stages of the ticks Amblyomma ovale (Larvae=26; Nymphs=10), Amblyomma sculptum (Larvae=86; Nymphs=6) and Ornithodoros sp. cf. O. mimon (Larvae=90) were also analyzed. DNA was extracted individually from blood samples and ticks. Molecular detection of Ehrlichia agents was performed targeting two different loci: 16S rRNA and dsb gen. The phylogenetic analyses showed that the Ehrlichia sp. detected in D. albiventris in this study is identical to Ehrlichia sp. strain Natal previously detected in two marsupials from Brazil. Furthermore, a new Ehrlichia strain was amplified from an A. ovale nymph (named as Ehrlichia sp. strain El Bagual) which is phylogenetically closely related to a strain of Ehrlichia sp. detected in Bradypus tridactylus in Brazil. The findings of the current study represent the first report of these two strains of Ehrlichia for Argentina, showing that the diversity of Ehrlichia spp. is greater than previously assumed. Further studies should determine the epidemiological relevance of these findings.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-21T11:13:57Z
2022-09-21T11:13:57Z
2022-09
info:eu-repo/date/embargoEnd/2023-09-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12919
https://www.sciencedirect.com/science/article/pii/S1877959X22001455
Evelina L. Tarragona, Fernando S. Flores, Daniela Lamattina, Jorgelina Torrents, Patrick S. Sebastian, Santiago Nava, Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina, Ticks and Tick-borne Diseases, 2022, 102043, ISSN 1877-959X, https://doi.org/10.1016/j.ttbdis.2022.102043.
1877-959X
https://doi.org/10.1016/j.ttbdis.2022.102043
url http://hdl.handle.net/20.500.12123/12919
https://www.sciencedirect.com/science/article/pii/S1877959X22001455
https://doi.org/10.1016/j.ttbdis.2022.102043
identifier_str_mv Evelina L. Tarragona, Fernando S. Flores, Daniela Lamattina, Jorgelina Torrents, Patrick S. Sebastian, Santiago Nava, Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina, Ticks and Tick-borne Diseases, 2022, 102043, ISSN 1877-959X, https://doi.org/10.1016/j.ttbdis.2022.102043.
1877-959X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ticks and Tick-borne Diseases : 102043 (November 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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