A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs

Autores
Obiegala, Anna; Król, Nina; Heyse, Lara M.I.; Pfeffer, Martin; Montini, Martina; Nava, Santiago; Sebastian, Patrick
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.
EEA Rafaela
Fil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Heyse, Lara M.I. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Montini, Martina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Experimental and Applied Acarology 94 : article number 57. (2025)
Materia
Amblyomma
Feeding Systems
Ticks
Laboratory Animals
Tick-borne Diseases
Sistema de Alimentación
Garrapatas
Animal de Laboratorio
Enfermedad Transmitida por Garrapatas
Amblyomma sculptum
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22576
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22576
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphsObiegala, AnnaKról, NinaHeyse, Lara M.I.Pfeffer, MartinMontini, MartinaNava, SantiagoSebastian, PatrickAmblyommaFeeding SystemsTicksLaboratory AnimalsTick-borne DiseasesSistema de AlimentaciónGarrapatasAnimal de LaboratorioEnfermedad Transmitida por GarrapatasAmblyomma sculptumThe aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.EEA RafaelaFil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Heyse, Lara M.I. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Montini, Martina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaSpringer2025-06-09T11:02:29Z2025-06-09T11:02:29Z2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22576https://link.springer.com/article/10.1007/s10493-025-01026-60168-81621572-9702https://doi.org/10.1007/s10493-025-01026-6Experimental and Applied Acarology 94 : article number 57. (2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:34Zoai:localhost:20.500.12123/22576instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:34.65INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
title A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
spellingShingle A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
Obiegala, Anna
Amblyomma
Feeding Systems
Ticks
Laboratory Animals
Tick-borne Diseases
Sistema de Alimentación
Garrapatas
Animal de Laboratorio
Enfermedad Transmitida por Garrapatas
Amblyomma sculptum
title_short A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
title_full A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
title_fullStr A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
title_full_unstemmed A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
title_sort A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
dc.creator.none.fl_str_mv Obiegala, Anna
Król, Nina
Heyse, Lara M.I.
Pfeffer, Martin
Montini, Martina
Nava, Santiago
Sebastian, Patrick
author Obiegala, Anna
author_facet Obiegala, Anna
Król, Nina
Heyse, Lara M.I.
Pfeffer, Martin
Montini, Martina
Nava, Santiago
Sebastian, Patrick
author_role author
author2 Król, Nina
Heyse, Lara M.I.
Pfeffer, Martin
Montini, Martina
Nava, Santiago
Sebastian, Patrick
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Amblyomma
Feeding Systems
Ticks
Laboratory Animals
Tick-borne Diseases
Sistema de Alimentación
Garrapatas
Animal de Laboratorio
Enfermedad Transmitida por Garrapatas
Amblyomma sculptum
topic Amblyomma
Feeding Systems
Ticks
Laboratory Animals
Tick-borne Diseases
Sistema de Alimentación
Garrapatas
Animal de Laboratorio
Enfermedad Transmitida por Garrapatas
Amblyomma sculptum
dc.description.none.fl_txt_mv The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.
EEA Rafaela
Fil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Heyse, Lara M.I. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Montini, Martina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.
publishDate 2025
dc.date.none.fl_str_mv 2025-06-09T11:02:29Z
2025-06-09T11:02:29Z
2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22576
https://link.springer.com/article/10.1007/s10493-025-01026-6
0168-8162
1572-9702
https://doi.org/10.1007/s10493-025-01026-6
url http://hdl.handle.net/20.500.12123/22576
https://link.springer.com/article/10.1007/s10493-025-01026-6
https://doi.org/10.1007/s10493-025-01026-6
identifier_str_mv 0168-8162
1572-9702
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Experimental and Applied Acarology 94 : article number 57. (2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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