A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
- Autores
- Obiegala, Anna; Król, Nina; Heyse, Lara M.I.; Pfeffer, Martin; Montini, Martina; Nava, Santiago; Sebastian, Patrick
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.
EEA Rafaela
Fil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Heyse, Lara M.I. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania
Fil: Montini, Martina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina - Fuente
- Experimental and Applied Acarology 94 : article number 57. (2025)
- Materia
-
Amblyomma
Feeding Systems
Ticks
Laboratory Animals
Tick-borne Diseases
Sistema de Alimentación
Garrapatas
Animal de Laboratorio
Enfermedad Transmitida por Garrapatas
Amblyomma sculptum - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/22576
Ver los metadatos del registro completo
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A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphsObiegala, AnnaKról, NinaHeyse, Lara M.I.Pfeffer, MartinMontini, MartinaNava, SantiagoSebastian, PatrickAmblyommaFeeding SystemsTicksLaboratory AnimalsTick-borne DiseasesSistema de AlimentaciónGarrapatasAnimal de LaboratorioEnfermedad Transmitida por GarrapatasAmblyomma sculptumThe aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.EEA RafaelaFil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Heyse, Lara M.I. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; AlemaniaFil: Montini, Martina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaSpringer2025-06-09T11:02:29Z2025-06-09T11:02:29Z2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22576https://link.springer.com/article/10.1007/s10493-025-01026-60168-81621572-9702https://doi.org/10.1007/s10493-025-01026-6Experimental and Applied Acarology 94 : article number 57. (2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:20Zoai:localhost:20.500.12123/22576instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:20.746INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
title |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
spellingShingle |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs Obiegala, Anna Amblyomma Feeding Systems Ticks Laboratory Animals Tick-borne Diseases Sistema de Alimentación Garrapatas Animal de Laboratorio Enfermedad Transmitida por Garrapatas Amblyomma sculptum |
title_short |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
title_full |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
title_fullStr |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
title_full_unstemmed |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
title_sort |
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
dc.creator.none.fl_str_mv |
Obiegala, Anna Król, Nina Heyse, Lara M.I. Pfeffer, Martin Montini, Martina Nava, Santiago Sebastian, Patrick |
author |
Obiegala, Anna |
author_facet |
Obiegala, Anna Król, Nina Heyse, Lara M.I. Pfeffer, Martin Montini, Martina Nava, Santiago Sebastian, Patrick |
author_role |
author |
author2 |
Król, Nina Heyse, Lara M.I. Pfeffer, Martin Montini, Martina Nava, Santiago Sebastian, Patrick |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Amblyomma Feeding Systems Ticks Laboratory Animals Tick-borne Diseases Sistema de Alimentación Garrapatas Animal de Laboratorio Enfermedad Transmitida por Garrapatas Amblyomma sculptum |
topic |
Amblyomma Feeding Systems Ticks Laboratory Animals Tick-borne Diseases Sistema de Alimentación Garrapatas Animal de Laboratorio Enfermedad Transmitida por Garrapatas Amblyomma sculptum |
dc.description.none.fl_txt_mv |
The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy. EEA Rafaela Fil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Heyse, Lara M.I. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Montini, Martina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina |
description |
The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-06-09T11:02:29Z 2025-06-09T11:02:29Z 2025-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/22576 https://link.springer.com/article/10.1007/s10493-025-01026-6 0168-8162 1572-9702 https://doi.org/10.1007/s10493-025-01026-6 |
url |
http://hdl.handle.net/20.500.12123/22576 https://link.springer.com/article/10.1007/s10493-025-01026-6 https://doi.org/10.1007/s10493-025-01026-6 |
identifier_str_mv |
0168-8162 1572-9702 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Experimental and Applied Acarology 94 : article number 57. (2025) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619204866605056 |
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12.559606 |