Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties

Autores
Dieguez, Maria Jose; Lopez, Micaela; Altieri, Emiliano; Pergolesi, María Fernanda; Dabove, Marisol Alicia; Cuyeu, Alba Romina; Justus, Nadia Alejandra; Kandus, Mariana Virginia; Ingala, Lorena Romina; Sacco, Francisco
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene LrBMP1 was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene LrSV1, the APR genetic system LrSV2 + LrcSV2 and the ASR (All Stage Resistance) gene LrG6. Using backcrosses, LrBMP1 was introgressed in four commercial susceptible varieties and LrSV1, LrSV2 + LrcSV2 and LrG6 were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system LrSV2 + LrcSV2 for leaf rust APR is reported.
Instituto de Genética
Fil: Dieguez, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lopez, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lopez, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Altieri, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pergolesi, Marí­a Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Dabove, Marisol Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Cuyeu, Alba Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Justus, Nadia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Kandus, Mariana Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
Biology 10 (11) : 1168 (Noviembre 2021)
Materia
Wheat
Marker-assisted Selection
Disease Resistance
Trigo
Selección Asistida por Marcadores
Resistencia a la Enfermedad
Wheat Leaf Rust
Introgression
Fine Mapping
Roya de la Hoja de Trigo
Introgresión
Mapeo Fino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15288
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oai_identifier_str oai:localhost:20.500.12123/15288
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network_name_str INTA Digital (INTA)
spelling Characterization and use in wheat breeding of leaf rust resistance genes from durable varietiesDieguez, Maria JoseLopez, MicaelaAltieri, EmilianoPergolesi, María FernandaDabove, Marisol AliciaCuyeu, Alba RominaJustus, Nadia AlejandraKandus, Mariana VirginiaIngala, Lorena RominaSacco, FranciscoWheatMarker-assisted SelectionDisease ResistanceTrigoSelección Asistida por MarcadoresResistencia a la EnfermedadWheat Leaf RustIntrogressionFine MappingRoya de la Hoja de TrigoIntrogresiónMapeo FinoLeaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene LrBMP1 was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene LrSV1, the APR genetic system LrSV2 + LrcSV2 and the ASR (All Stage Resistance) gene LrG6. Using backcrosses, LrBMP1 was introgressed in four commercial susceptible varieties and LrSV1, LrSV2 + LrcSV2 and LrG6 were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system LrSV2 + LrcSV2 for leaf rust APR is reported.Instituto de GenéticaFil: Dieguez, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Lopez, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Lopez, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Altieri, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Pergolesi, Marí­a Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Dabove, Marisol Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Cuyeu, Alba Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Justus, Nadia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Kandus, Mariana Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaMDPI2023-09-22T10:38:52Z2023-09-22T10:38:52Z2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15288https://www.mdpi.com/2079-7737/10/11/11682079-7737https://doi.org/10.3390/biology10111168Biology 10 (11) : 1168 (Noviembre 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I085-001, Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.info:eu-repograntAgreement/INTA/2019-PE-E6-I126-001, Mejoramiento genético de trigo pan (Triticum aestivum L.) y trigo candeal (Triticum turgidum ssp. durum Desf.)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:06Zoai:localhost:20.500.12123/15288instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:07.229INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
title Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
spellingShingle Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
Dieguez, Maria Jose
Wheat
Marker-assisted Selection
Disease Resistance
Trigo
Selección Asistida por Marcadores
Resistencia a la Enfermedad
Wheat Leaf Rust
Introgression
Fine Mapping
Roya de la Hoja de Trigo
Introgresión
Mapeo Fino
title_short Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
title_full Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
title_fullStr Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
title_full_unstemmed Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
title_sort Characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
dc.creator.none.fl_str_mv Dieguez, Maria Jose
Lopez, Micaela
Altieri, Emiliano
Pergolesi, María Fernanda
Dabove, Marisol Alicia
Cuyeu, Alba Romina
Justus, Nadia Alejandra
Kandus, Mariana Virginia
Ingala, Lorena Romina
Sacco, Francisco
author Dieguez, Maria Jose
author_facet Dieguez, Maria Jose
Lopez, Micaela
Altieri, Emiliano
Pergolesi, María Fernanda
Dabove, Marisol Alicia
Cuyeu, Alba Romina
Justus, Nadia Alejandra
Kandus, Mariana Virginia
Ingala, Lorena Romina
Sacco, Francisco
author_role author
author2 Lopez, Micaela
Altieri, Emiliano
Pergolesi, María Fernanda
Dabove, Marisol Alicia
Cuyeu, Alba Romina
Justus, Nadia Alejandra
Kandus, Mariana Virginia
Ingala, Lorena Romina
Sacco, Francisco
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Wheat
Marker-assisted Selection
Disease Resistance
Trigo
Selección Asistida por Marcadores
Resistencia a la Enfermedad
Wheat Leaf Rust
Introgression
Fine Mapping
Roya de la Hoja de Trigo
Introgresión
Mapeo Fino
topic Wheat
Marker-assisted Selection
Disease Resistance
Trigo
Selección Asistida por Marcadores
Resistencia a la Enfermedad
Wheat Leaf Rust
Introgression
Fine Mapping
Roya de la Hoja de Trigo
Introgresión
Mapeo Fino
dc.description.none.fl_txt_mv Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene LrBMP1 was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene LrSV1, the APR genetic system LrSV2 + LrcSV2 and the ASR (All Stage Resistance) gene LrG6. Using backcrosses, LrBMP1 was introgressed in four commercial susceptible varieties and LrSV1, LrSV2 + LrcSV2 and LrG6 were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system LrSV2 + LrcSV2 for leaf rust APR is reported.
Instituto de Genética
Fil: Dieguez, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lopez, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lopez, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Altieri, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pergolesi, Marí­a Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Dabove, Marisol Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Cuyeu, Alba Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Justus, Nadia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Kandus, Mariana Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene LrBMP1 was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene LrSV1, the APR genetic system LrSV2 + LrcSV2 and the ASR (All Stage Resistance) gene LrG6. Using backcrosses, LrBMP1 was introgressed in four commercial susceptible varieties and LrSV1, LrSV2 + LrcSV2 and LrG6 were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system LrSV2 + LrcSV2 for leaf rust APR is reported.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
2023-09-22T10:38:52Z
2023-09-22T10:38:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15288
https://www.mdpi.com/2079-7737/10/11/1168
2079-7737
https://doi.org/10.3390/biology10111168
url http://hdl.handle.net/20.500.12123/15288
https://www.mdpi.com/2079-7737/10/11/1168
https://doi.org/10.3390/biology10111168
identifier_str_mv 2079-7737
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001, Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.
info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.
info:eu-repograntAgreement/INTA/2019-PE-E6-I126-001, Mejoramiento genético de trigo pan (Triticum aestivum L.) y trigo candeal (Triticum turgidum ssp. durum Desf.)
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Biology 10 (11) : 1168 (Noviembre 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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