Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
- Autores
- Diéguez, M. J.; Pergolesi, M. F.; Velásquez, Silvia Melina; Ingala, Lorena Romina; López, M.; Darino, Martin Alejandro; Paux, E.; Feuillet, C.; Sacco, F.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.
Fil: Diéguez, M. J.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Pergolesi, M. F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: López, M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Darino, Martin Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Paux, E.. Institut National de la Recherche Agronomique; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Feuillet, C.. Institut National de la Recherche Agronomique; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Sacco, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina - Materia
-
Weath
Rust
Fine Mapping
Puccinia - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/32587
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/32587 |
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Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BSDiéguez, M. J.Pergolesi, M. F.Velásquez, Silvia MelinaIngala, Lorena RominaLópez, M.Darino, Martin AlejandroPaux, E.Feuillet, C.Sacco, F.WeathRustFine MappingPucciniahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.Fil: Diéguez, M. J.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Pergolesi, M. F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: López, M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Darino, Martin Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Paux, E.. Institut National de la Recherche Agronomique; Francia. Centre National de la Recherche Scientifique; FranciaFil: Feuillet, C.. Institut National de la Recherche Agronomique; Francia. Centre National de la Recherche Scientifique; FranciaFil: Sacco, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaSpringer2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32587Feuillet, C.; Paux, E.; Darino, Martin Alejandro; Ingala, Lorena Romina; Velásquez, Silvia Melina; Pergolesi, M. F.; et al.; Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS; Springer; Theoretical And Applied Genetics; 127; 5; 2-2014; 1133-11410040-57521432-2242CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00122-014-2285-zinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00122-014-2285-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:19Zoai:ri.conicet.gov.ar:11336/32587instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:19.873CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
spellingShingle |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS Diéguez, M. J. Weath Rust Fine Mapping Puccinia |
title_short |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_full |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_fullStr |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_full_unstemmed |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_sort |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
dc.creator.none.fl_str_mv |
Diéguez, M. J. Pergolesi, M. F. Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, E. Feuillet, C. Sacco, F. |
author |
Diéguez, M. J. |
author_facet |
Diéguez, M. J. Pergolesi, M. F. Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, E. Feuillet, C. Sacco, F. |
author_role |
author |
author2 |
Pergolesi, M. F. Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, E. Feuillet, C. Sacco, F. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Weath Rust Fine Mapping Puccinia |
topic |
Weath Rust Fine Mapping Puccinia |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. Fil: Diéguez, M. J.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina Fil: Pergolesi, M. F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina Fil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: López, M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina Fil: Darino, Martin Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina Fil: Paux, E.. Institut National de la Recherche Agronomique; Francia. Centre National de la Recherche Scientifique; Francia Fil: Feuillet, C.. Institut National de la Recherche Agronomique; Francia. Centre National de la Recherche Scientifique; Francia Fil: Sacco, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina |
description |
Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/32587 Feuillet, C.; Paux, E.; Darino, Martin Alejandro; Ingala, Lorena Romina; Velásquez, Silvia Melina; Pergolesi, M. F.; et al.; Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS; Springer; Theoretical And Applied Genetics; 127; 5; 2-2014; 1133-1141 0040-5752 1432-2242 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/32587 |
identifier_str_mv |
Feuillet, C.; Paux, E.; Darino, Martin Alejandro; Ingala, Lorena Romina; Velásquez, Silvia Melina; Pergolesi, M. F.; et al.; Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS; Springer; Theoretical And Applied Genetics; 127; 5; 2-2014; 1133-1141 0040-5752 1432-2242 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00122-014-2285-z info:eu-repo/semantics/altIdentifier/doi/10.1007/s00122-014-2285-z |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613447500693504 |
score |
13.070432 |