Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage

Autores
Vera, F.; Craig, M. I.; Olivera, V.; Rojas, F.; König, Guido Alberto; Pereda, Ariel Julián; Vagnozzi, Ariel Eduardo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
Fil: Vera, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Area de Producción Animal; Argentina
Fil: Craig, M. I.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; Argentina
Fil: Olivera, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Rojas, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: König, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereda, Ariel Julián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Materia
Infectious Bursal Disease Virus
Nucleotide Sequence Identity
Regional Lineage
Amino Acid Sequence Analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38446
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38446
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineageVera, F.Craig, M. I.Olivera, V.Rojas, F.König, Guido AlbertoPereda, Ariel JuliánVagnozzi, Ariel EduardoInfectious Bursal Disease VirusNucleotide Sequence IdentityRegional LineageAmino Acid Sequence AnalysisIn Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.Fil: Vera, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Area de Producción Animal; ArgentinaFil: Craig, M. I.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; ArgentinaFil: Olivera, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Rojas, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; ArgentinaFil: König, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pereda, Ariel Julián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaSpringer Wien2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38446Vera, F.; Craig, M. I.; Olivera, V.; Rojas, F.; König, Guido Alberto; et al.; Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage; Springer Wien; Archives of Virology; 160; 8; 8-2015; 1909-19210304-8608CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00705-015-2449-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s00705-015-2449-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:52:25Zoai:ri.conicet.gov.ar:11336/38446instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:52:26.178CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
spellingShingle Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
Vera, F.
Infectious Bursal Disease Virus
Nucleotide Sequence Identity
Regional Lineage
Amino Acid Sequence Analysis
title_short Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_full Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_fullStr Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_full_unstemmed Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_sort Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
dc.creator.none.fl_str_mv Vera, F.
Craig, M. I.
Olivera, V.
Rojas, F.
König, Guido Alberto
Pereda, Ariel Julián
Vagnozzi, Ariel Eduardo
author Vera, F.
author_facet Vera, F.
Craig, M. I.
Olivera, V.
Rojas, F.
König, Guido Alberto
Pereda, Ariel Julián
Vagnozzi, Ariel Eduardo
author_role author
author2 Craig, M. I.
Olivera, V.
Rojas, F.
König, Guido Alberto
Pereda, Ariel Julián
Vagnozzi, Ariel Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Infectious Bursal Disease Virus
Nucleotide Sequence Identity
Regional Lineage
Amino Acid Sequence Analysis
topic Infectious Bursal Disease Virus
Nucleotide Sequence Identity
Regional Lineage
Amino Acid Sequence Analysis
dc.description.none.fl_txt_mv In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
Fil: Vera, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Area de Producción Animal; Argentina
Fil: Craig, M. I.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; Argentina
Fil: Olivera, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Rojas, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: König, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereda, Ariel Julián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
description In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38446
Vera, F.; Craig, M. I.; Olivera, V.; Rojas, F.; König, Guido Alberto; et al.; Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage; Springer Wien; Archives of Virology; 160; 8; 8-2015; 1909-1921
0304-8608
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38446
identifier_str_mv Vera, F.; Craig, M. I.; Olivera, V.; Rojas, F.; König, Guido Alberto; et al.; Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage; Springer Wien; Archives of Virology; 160; 8; 8-2015; 1909-1921
0304-8608
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00705-015-2449-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00705-015-2449-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Wien
publisher.none.fl_str_mv Springer Wien
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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