Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season

Autores
Pin Viso, Natalia Daniela; Ortiz, Jimena; Maury, Mariana; Frene, Juan Pablo; Iocoli, Gastón Alejandro; Lorenzon, Claudio Antonio; Rivarola, Maximo Lisandro; García, Fernando O.; Gudelj, Vicente Jorge; Faggioli, Valeria Soledad
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Among agronomical practices, fertilisation greatly influences soil microbial diversity and functionality and severely disturbs the global nitrogen (N) cycle. In this study, we assessed the effect of 16 years of mineral fertilisation (NPS) on soil bacterial-archaeal communities in a no-tillage experiment in a humid climate season. We used 16S rRNA amplicon sequencing and functional prediction of marker genes involved in the N cycle at two soil depths (0–5 and 5–10 cm). Samples were taken after soybean harvest in a wheat/soybean-maize crop sequence. At both soil depths, fertilisation increased the abundance of Proteobacteria while reducing the abundance of Verrucomicrobiota. Indicator species analyses revealed that MB-A2–108 (Actinobacteriota) and Nitrososphaeraceae (Archaea) were indicators of the low fertiliser rates at 0–5 cm. Instead, Rokubacteriales (Methylomirabilota) and Nitrolancea (Chloroflexi) were indicators of low and high rates, respectively, at 5–10 cm. Biological N–fixation genes (nifA, nifV nifHD1, and nifHD2) showed an inconsistent response depending on the soil depth. Yet, the high abundance of nifA revealed the presence of N-fixing microorganisms even at high levels of N fertilisation. The predominance of genes involved in the dissimilatory (nirB and nirK) and assimilatory (nirA) reduction of nitrate was steadily found at higher fertilisation. Given that the predecessor crop was a legume that did not receive N, our results revealed the substantial legacy of long-term N inputs on soil bacterial-archaeal diversity and N-cycling genes that might have been umpired by the current humid conditions.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Ortiz, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Maury, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Frene, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Iocoli, Gastón Alejandro. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Lorenzón, Claudio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García, Fernando O. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Gudelj, Vicente Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fuente
Applied Soil Ecology 193 : 105149 (January 2024)
Materia
Suelo
Aplicación de Abonos
Microorganismos del Suelo
Soil
Fertilizer Application
Soil Microorganisms
Soil Microbiota
Mineral Fertilisation
Nitrogen Cycle
Fertilización
Inoculantes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16474
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16474
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop seasonPin Viso, Natalia DanielaOrtiz, JimenaMaury, MarianaFrene, Juan PabloIocoli, Gastón AlejandroLorenzon, Claudio AntonioRivarola, Maximo LisandroGarcía, Fernando O.Gudelj, Vicente JorgeFaggioli, Valeria SoledadSueloAplicación de AbonosMicroorganismos del SueloSoilFertilizer ApplicationSoil MicroorganismsSoil MicrobiotaMineral FertilisationNitrogen CycleFertilizaciónInoculantesAmong agronomical practices, fertilisation greatly influences soil microbial diversity and functionality and severely disturbs the global nitrogen (N) cycle. In this study, we assessed the effect of 16 years of mineral fertilisation (NPS) on soil bacterial-archaeal communities in a no-tillage experiment in a humid climate season. We used 16S rRNA amplicon sequencing and functional prediction of marker genes involved in the N cycle at two soil depths (0–5 and 5–10 cm). Samples were taken after soybean harvest in a wheat/soybean-maize crop sequence. At both soil depths, fertilisation increased the abundance of Proteobacteria while reducing the abundance of Verrucomicrobiota. Indicator species analyses revealed that MB-A2–108 (Actinobacteriota) and Nitrososphaeraceae (Archaea) were indicators of the low fertiliser rates at 0–5 cm. Instead, Rokubacteriales (Methylomirabilota) and Nitrolancea (Chloroflexi) were indicators of low and high rates, respectively, at 5–10 cm. Biological N–fixation genes (nifA, nifV nifHD1, and nifHD2) showed an inconsistent response depending on the soil depth. Yet, the high abundance of nifA revealed the presence of N-fixing microorganisms even at high levels of N fertilisation. The predominance of genes involved in the dissimilatory (nirB and nirK) and assimilatory (nirA) reduction of nitrate was steadily found at higher fertilisation. Given that the predecessor crop was a legume that did not receive N, our results revealed the substantial legacy of long-term N inputs on soil bacterial-archaeal diversity and N-cycling genes that might have been umpired by the current humid conditions.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Ortiz, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Maury, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Frene, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Iocoli, Gastón Alejandro. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Lorenzón, Claudio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García, Fernando O. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Gudelj, Vicente Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaElsevier2024-01-08T17:39:23Z2024-01-08T17:39:23Z2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16474https://www.sciencedirect.com/science/article/pii/S09291393230034750929-13931873-0272https://doi.org/10.1016/j.apsoil.2023.105149Applied Soil Ecology 193 : 105149 (January 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNCYO-1127033/AR./Manejo nutricional de cereales y oleaginosas para la intensificación sustentable de los sistemas productivosinfo:eu-repograntAgreement/INTA/PNCER-2342/AR./Diagnóstico, Reposición de nutrientes y tecnología de fertilizacióninfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:18Zoai:localhost:20.500.12123/16474instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:18.639INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
title Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
spellingShingle Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
Pin Viso, Natalia Daniela
Suelo
Aplicación de Abonos
Microorganismos del Suelo
Soil
Fertilizer Application
Soil Microorganisms
Soil Microbiota
Mineral Fertilisation
Nitrogen Cycle
Fertilización
Inoculantes
title_short Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
title_full Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
title_fullStr Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
title_full_unstemmed Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
title_sort Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
dc.creator.none.fl_str_mv Pin Viso, Natalia Daniela
Ortiz, Jimena
Maury, Mariana
Frene, Juan Pablo
Iocoli, Gastón Alejandro
Lorenzon, Claudio Antonio
Rivarola, Maximo Lisandro
García, Fernando O.
Gudelj, Vicente Jorge
Faggioli, Valeria Soledad
author Pin Viso, Natalia Daniela
author_facet Pin Viso, Natalia Daniela
Ortiz, Jimena
Maury, Mariana
Frene, Juan Pablo
Iocoli, Gastón Alejandro
Lorenzon, Claudio Antonio
Rivarola, Maximo Lisandro
García, Fernando O.
Gudelj, Vicente Jorge
Faggioli, Valeria Soledad
author_role author
author2 Ortiz, Jimena
Maury, Mariana
Frene, Juan Pablo
Iocoli, Gastón Alejandro
Lorenzon, Claudio Antonio
Rivarola, Maximo Lisandro
García, Fernando O.
Gudelj, Vicente Jorge
Faggioli, Valeria Soledad
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Suelo
Aplicación de Abonos
Microorganismos del Suelo
Soil
Fertilizer Application
Soil Microorganisms
Soil Microbiota
Mineral Fertilisation
Nitrogen Cycle
Fertilización
Inoculantes
topic Suelo
Aplicación de Abonos
Microorganismos del Suelo
Soil
Fertilizer Application
Soil Microorganisms
Soil Microbiota
Mineral Fertilisation
Nitrogen Cycle
Fertilización
Inoculantes
dc.description.none.fl_txt_mv Among agronomical practices, fertilisation greatly influences soil microbial diversity and functionality and severely disturbs the global nitrogen (N) cycle. In this study, we assessed the effect of 16 years of mineral fertilisation (NPS) on soil bacterial-archaeal communities in a no-tillage experiment in a humid climate season. We used 16S rRNA amplicon sequencing and functional prediction of marker genes involved in the N cycle at two soil depths (0–5 and 5–10 cm). Samples were taken after soybean harvest in a wheat/soybean-maize crop sequence. At both soil depths, fertilisation increased the abundance of Proteobacteria while reducing the abundance of Verrucomicrobiota. Indicator species analyses revealed that MB-A2–108 (Actinobacteriota) and Nitrososphaeraceae (Archaea) were indicators of the low fertiliser rates at 0–5 cm. Instead, Rokubacteriales (Methylomirabilota) and Nitrolancea (Chloroflexi) were indicators of low and high rates, respectively, at 5–10 cm. Biological N–fixation genes (nifA, nifV nifHD1, and nifHD2) showed an inconsistent response depending on the soil depth. Yet, the high abundance of nifA revealed the presence of N-fixing microorganisms even at high levels of N fertilisation. The predominance of genes involved in the dissimilatory (nirB and nirK) and assimilatory (nirA) reduction of nitrate was steadily found at higher fertilisation. Given that the predecessor crop was a legume that did not receive N, our results revealed the substantial legacy of long-term N inputs on soil bacterial-archaeal diversity and N-cycling genes that might have been umpired by the current humid conditions.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Ortiz, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Maury, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Frene, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Iocoli, Gastón Alejandro. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Lorenzón, Claudio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García, Fernando O. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Gudelj, Vicente Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
description Among agronomical practices, fertilisation greatly influences soil microbial diversity and functionality and severely disturbs the global nitrogen (N) cycle. In this study, we assessed the effect of 16 years of mineral fertilisation (NPS) on soil bacterial-archaeal communities in a no-tillage experiment in a humid climate season. We used 16S rRNA amplicon sequencing and functional prediction of marker genes involved in the N cycle at two soil depths (0–5 and 5–10 cm). Samples were taken after soybean harvest in a wheat/soybean-maize crop sequence. At both soil depths, fertilisation increased the abundance of Proteobacteria while reducing the abundance of Verrucomicrobiota. Indicator species analyses revealed that MB-A2–108 (Actinobacteriota) and Nitrososphaeraceae (Archaea) were indicators of the low fertiliser rates at 0–5 cm. Instead, Rokubacteriales (Methylomirabilota) and Nitrolancea (Chloroflexi) were indicators of low and high rates, respectively, at 5–10 cm. Biological N–fixation genes (nifA, nifV nifHD1, and nifHD2) showed an inconsistent response depending on the soil depth. Yet, the high abundance of nifA revealed the presence of N-fixing microorganisms even at high levels of N fertilisation. The predominance of genes involved in the dissimilatory (nirB and nirK) and assimilatory (nirA) reduction of nitrate was steadily found at higher fertilisation. Given that the predecessor crop was a legume that did not receive N, our results revealed the substantial legacy of long-term N inputs on soil bacterial-archaeal diversity and N-cycling genes that might have been umpired by the current humid conditions.
publishDate 2024
dc.date.none.fl_str_mv 2024-01-08T17:39:23Z
2024-01-08T17:39:23Z
2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16474
https://www.sciencedirect.com/science/article/pii/S0929139323003475
0929-1393
1873-0272
https://doi.org/10.1016/j.apsoil.2023.105149
url http://hdl.handle.net/20.500.12123/16474
https://www.sciencedirect.com/science/article/pii/S0929139323003475
https://doi.org/10.1016/j.apsoil.2023.105149
identifier_str_mv 0929-1393
1873-0272
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNCYO-1127033/AR./Manejo nutricional de cereales y oleaginosas para la intensificación sustentable de los sistemas productivos
info:eu-repograntAgreement/INTA/PNCER-2342/AR./Diagnóstico, Reposición de nutrientes y tecnología de fertilización
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Applied Soil Ecology 193 : 105149 (January 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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