Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas

Autores
Vasquez-Espejo, Carina; Sardo, María Florencia; Álvarez-Aliaga, M. Teresa; Monteoliva, Mariela Ines; Valetti, Lucio; Páez, Paulina L.
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ascochyta blight (caused by Ascochyta rabiei infection) is a devastating disease in chickpea crops. Farmers apply fungicides several times once symptoms appear, with poor control efficacy. Chemical fungicides, despite their widespread use, have harmful effects and an environmental impact. The lack of control strategies, plus the impact of excessive fungicide use, underscores the need to explore novel and sustainable biocontrol alternatives. Furthermore, the stability and bioavailability of fungicides could be improved by a formulation as nanoparticles (NPs), by chemical or biological synthesis. While the chemical synthesis requires harmful chemicals and high costs, the biological synthesis uses biological components as reducing and stabilizing agents. Then, the biological synthesis minimizes harmful chemicals and adverse effects on humans, animals, and the environment, in alignment with the “One Health” approach. The nanoscale size is key, as it increases the surface area for contact with the target, thus enhancing the efficacy. The objective of this study was to evaluate the antifungal activity of biosynthesized copper nanoparticles (CuNPs) against A. rabiei. The biosynthesized CuNPs (CuNPs2 and CuNPs3) exhibited significantly greater inhibition compared to copper sulfate salt. They showed in vitro inhibition as much as twice that of the copper salt. CuNPs2 and CuNPs3 successfully reduced the disease incidence in chickpea leaves by > 60 % demonstrating their potential as an alternative biocontrol strategy. The superior efficacy of CuNPs was attributed to their nanoscale properties and biogenic synthesis method. The use of bioderived CuNPs represents a promising and cost-effective alternative to traditional fungicides, advocating for more sustainable agricultural practices.
Instituto de Patología Vegetal
Fil: Vasquez-Espejo, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); Argentina
Fil: Sardo, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Sardo, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Álvarez-Aliaga, M. Teresa. Universidad Mayor de San Andrés. Facultad de Ciencias Farmacéuticas y Bioquímicas. Instituto de Investigaciones Fármaco Bioquímicas.Área de Bioquímica Molecular; Bolivia
Fil: Monteoliva, Mariela Ines . Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Monteoliva, Mariela Ines . Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Páez, Paulina L. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina
Fil: Páez, Paulina L. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); Argentina
Fuente
Plant Nano Biology 15 : 100222. (February 2026)
Materia
Biosynthesis
Chickpeas
Plant Diseases
Chickpea Blight
Nanoparticles
Biosíntesis
Garbanzo
Enfermedades de las Plantas
Tizón del Garbanzo
Nanopartículas
Bacterial Strains
Copper Nanoparticles
Antifungal Activity
Ascochyta Blight
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26298
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network_name_str INTA Digital (INTA)
spelling Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeasVasquez-Espejo, CarinaSardo, María FlorenciaÁlvarez-Aliaga, M. TeresaMonteoliva, Mariela InesValetti, LucioPáez, Paulina L.BiosynthesisChickpeasPlant DiseasesChickpea BlightNanoparticlesBiosíntesisGarbanzoEnfermedades de las PlantasTizón del GarbanzoNanopartículasBacterial StrainsCopper NanoparticlesAntifungal ActivityAscochyta BlightAscochyta blight (caused by Ascochyta rabiei infection) is a devastating disease in chickpea crops. Farmers apply fungicides several times once symptoms appear, with poor control efficacy. Chemical fungicides, despite their widespread use, have harmful effects and an environmental impact. The lack of control strategies, plus the impact of excessive fungicide use, underscores the need to explore novel and sustainable biocontrol alternatives. Furthermore, the stability and bioavailability of fungicides could be improved by a formulation as nanoparticles (NPs), by chemical or biological synthesis. While the chemical synthesis requires harmful chemicals and high costs, the biological synthesis uses biological components as reducing and stabilizing agents. Then, the biological synthesis minimizes harmful chemicals and adverse effects on humans, animals, and the environment, in alignment with the “One Health” approach. The nanoscale size is key, as it increases the surface area for contact with the target, thus enhancing the efficacy. The objective of this study was to evaluate the antifungal activity of biosynthesized copper nanoparticles (CuNPs) against A. rabiei. The biosynthesized CuNPs (CuNPs2 and CuNPs3) exhibited significantly greater inhibition compared to copper sulfate salt. They showed in vitro inhibition as much as twice that of the copper salt. CuNPs2 and CuNPs3 successfully reduced the disease incidence in chickpea leaves by > 60 % demonstrating their potential as an alternative biocontrol strategy. The superior efficacy of CuNPs was attributed to their nanoscale properties and biogenic synthesis method. The use of bioderived CuNPs represents a promising and cost-effective alternative to traditional fungicides, advocating for more sustainable agricultural practices.Instituto de Patología VegetalFil: Vasquez-Espejo, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); ArgentinaFil: Sardo, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Sardo, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Álvarez-Aliaga, M. Teresa. Universidad Mayor de San Andrés. Facultad de Ciencias Farmacéuticas y Bioquímicas. Instituto de Investigaciones Fármaco Bioquímicas.Área de Bioquímica Molecular; BoliviaFil: Monteoliva, Mariela Ines . Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Monteoliva, Mariela Ines . Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Páez, Paulina L. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; ArgentinaFil: Páez, Paulina L. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); ArgentinaElsevier2026-05-21T10:14:47Z2026-05-21T10:14:47Z2026-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26298https://www.sciencedirect.com/science/article/pii/S27731111250008902773-1111https://doi.org/10.1016/j.plana.2025.100222Plant Nano Biology 15 : 100222. (February 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetalinfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestalesinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-06-04T09:46:23Zoai:localhost:20.500.12123/26298instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-06-04 09:46:24.645INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
title Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
spellingShingle Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
Vasquez-Espejo, Carina
Biosynthesis
Chickpeas
Plant Diseases
Chickpea Blight
Nanoparticles
Biosíntesis
Garbanzo
Enfermedades de las Plantas
Tizón del Garbanzo
Nanopartículas
Bacterial Strains
Copper Nanoparticles
Antifungal Activity
Ascochyta Blight
title_short Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
title_full Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
title_fullStr Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
title_full_unstemmed Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
title_sort Biogenic copper nanoparticles as a novel biocontrol strategy for Ascochyta blight in chickpeas
dc.creator.none.fl_str_mv Vasquez-Espejo, Carina
Sardo, María Florencia
Álvarez-Aliaga, M. Teresa
Monteoliva, Mariela Ines
Valetti, Lucio
Páez, Paulina L.
author Vasquez-Espejo, Carina
author_facet Vasquez-Espejo, Carina
Sardo, María Florencia
Álvarez-Aliaga, M. Teresa
Monteoliva, Mariela Ines
Valetti, Lucio
Páez, Paulina L.
author_role author
author2 Sardo, María Florencia
Álvarez-Aliaga, M. Teresa
Monteoliva, Mariela Ines
Valetti, Lucio
Páez, Paulina L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biosynthesis
Chickpeas
Plant Diseases
Chickpea Blight
Nanoparticles
Biosíntesis
Garbanzo
Enfermedades de las Plantas
Tizón del Garbanzo
Nanopartículas
Bacterial Strains
Copper Nanoparticles
Antifungal Activity
Ascochyta Blight
topic Biosynthesis
Chickpeas
Plant Diseases
Chickpea Blight
Nanoparticles
Biosíntesis
Garbanzo
Enfermedades de las Plantas
Tizón del Garbanzo
Nanopartículas
Bacterial Strains
Copper Nanoparticles
Antifungal Activity
Ascochyta Blight
dc.description.none.fl_txt_mv Ascochyta blight (caused by Ascochyta rabiei infection) is a devastating disease in chickpea crops. Farmers apply fungicides several times once symptoms appear, with poor control efficacy. Chemical fungicides, despite their widespread use, have harmful effects and an environmental impact. The lack of control strategies, plus the impact of excessive fungicide use, underscores the need to explore novel and sustainable biocontrol alternatives. Furthermore, the stability and bioavailability of fungicides could be improved by a formulation as nanoparticles (NPs), by chemical or biological synthesis. While the chemical synthesis requires harmful chemicals and high costs, the biological synthesis uses biological components as reducing and stabilizing agents. Then, the biological synthesis minimizes harmful chemicals and adverse effects on humans, animals, and the environment, in alignment with the “One Health” approach. The nanoscale size is key, as it increases the surface area for contact with the target, thus enhancing the efficacy. The objective of this study was to evaluate the antifungal activity of biosynthesized copper nanoparticles (CuNPs) against A. rabiei. The biosynthesized CuNPs (CuNPs2 and CuNPs3) exhibited significantly greater inhibition compared to copper sulfate salt. They showed in vitro inhibition as much as twice that of the copper salt. CuNPs2 and CuNPs3 successfully reduced the disease incidence in chickpea leaves by > 60 % demonstrating their potential as an alternative biocontrol strategy. The superior efficacy of CuNPs was attributed to their nanoscale properties and biogenic synthesis method. The use of bioderived CuNPs represents a promising and cost-effective alternative to traditional fungicides, advocating for more sustainable agricultural practices.
Instituto de Patología Vegetal
Fil: Vasquez-Espejo, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); Argentina
Fil: Sardo, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Sardo, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Álvarez-Aliaga, M. Teresa. Universidad Mayor de San Andrés. Facultad de Ciencias Farmacéuticas y Bioquímicas. Instituto de Investigaciones Fármaco Bioquímicas.Área de Bioquímica Molecular; Bolivia
Fil: Monteoliva, Mariela Ines . Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Monteoliva, Mariela Ines . Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Páez, Paulina L. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina
Fil: Páez, Paulina L. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); Argentina
description Ascochyta blight (caused by Ascochyta rabiei infection) is a devastating disease in chickpea crops. Farmers apply fungicides several times once symptoms appear, with poor control efficacy. Chemical fungicides, despite their widespread use, have harmful effects and an environmental impact. The lack of control strategies, plus the impact of excessive fungicide use, underscores the need to explore novel and sustainable biocontrol alternatives. Furthermore, the stability and bioavailability of fungicides could be improved by a formulation as nanoparticles (NPs), by chemical or biological synthesis. While the chemical synthesis requires harmful chemicals and high costs, the biological synthesis uses biological components as reducing and stabilizing agents. Then, the biological synthesis minimizes harmful chemicals and adverse effects on humans, animals, and the environment, in alignment with the “One Health” approach. The nanoscale size is key, as it increases the surface area for contact with the target, thus enhancing the efficacy. The objective of this study was to evaluate the antifungal activity of biosynthesized copper nanoparticles (CuNPs) against A. rabiei. The biosynthesized CuNPs (CuNPs2 and CuNPs3) exhibited significantly greater inhibition compared to copper sulfate salt. They showed in vitro inhibition as much as twice that of the copper salt. CuNPs2 and CuNPs3 successfully reduced the disease incidence in chickpea leaves by > 60 % demonstrating their potential as an alternative biocontrol strategy. The superior efficacy of CuNPs was attributed to their nanoscale properties and biogenic synthesis method. The use of bioderived CuNPs represents a promising and cost-effective alternative to traditional fungicides, advocating for more sustainable agricultural practices.
publishDate 2026
dc.date.none.fl_str_mv 2026-05-21T10:14:47Z
2026-05-21T10:14:47Z
2026-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26298
https://www.sciencedirect.com/science/article/pii/S2773111125000890
2773-1111
https://doi.org/10.1016/j.plana.2025.100222
url http://hdl.handle.net/20.500.12123/26298
https://www.sciencedirect.com/science/article/pii/S2773111125000890
https://doi.org/10.1016/j.plana.2025.100222
identifier_str_mv 2773-1111
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal
info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Plant Nano Biology 15 : 100222. (February 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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