Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker

Autores
Valetti, Lucio; Bernanrdi Lima, Nelson; Cazon, Luis Ignacio; Crociara, Clara Sonia; Ortega, Leandro Ismael; Pastor, Silvina Estela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apple valsa canker, caused by the fungus Valsa ceratosperma, is one of the most destructive diseases of this crop. Conventional fungicide treatments are not effective enough; therefore, it is necessary to develop alternatives for the control of this pathogen. The use of Trichoderma strains as a biocontrol agent is a promising strategy for the environmentally friendly management of this disease. In this study, seven different isolates of Trichoderma spp. were obtained from the rhizospheric soil of healthy apple plants. The antagonistic capacity of the isolates against the pathogen V. ceratosperma was evaluated using the dual culture method and by the production of antibiotic non-volatile compounds. In the dual culture method, the interaction zone was observed by differential interference contrast microscopy and confocal laser scanning microscopy. The isolates RN-13, RN-33, and RN-34 inhibited more than 75% of mycelial growth, whereas RN-19, RN-16, and RN-15 inhibited growth by 65–72% with respect to the control. The mycoparasitic activity was evidenced by the coiling of hyphae around pathogen hyphae, appressorium-like structures, morphological deformations, and disintegration of mycelial walls. Apple shoots inoculated with Trichoderma isolates and then infected with V. ceratosperma showed the antagonist capacity of RN-16, RN-19, RN-15, and RN-18, which produced a significant decrease in the size of the lesion. Overall, the results showed the great potential of Trichoderma as a biocontrol agent against apple tree valsa canker. Further studies of this fungal interaction are required to design effective control strategies for the protection of apple tree orchards from this disease.
Instituto de Patología Vegetal
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernanrdi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cazon, Luis Ignacio. Universidad Federal de Vicosa. Laboratorio de Epidemiología. Departamento de Fitopatología; Brasil
Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Ortega, Leandro Ismael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
European Journal of Plant Pathology 163 : 923–935 (2022)
Materia
Antagonism
Trichoderma
Apples
Biological Control Agents
Antagonismo
Valsa
Manzana
Agentes de Control Biológico
Biocontrol Agents
Canker Disease
Mycoparasitism
Valsa ceratosperma
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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Acceder
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oai_identifier_str oai:localhost:20.500.12123/13082
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network_name_str INTA Digital (INTA)
spelling Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa cankerValetti, LucioBernanrdi Lima, NelsonCazon, Luis IgnacioCrociara, Clara SoniaOrtega, Leandro IsmaelPastor, Silvina EstelaAntagonismTrichodermaApplesBiological Control AgentsAntagonismoValsaManzanaAgentes de Control BiológicoBiocontrol AgentsCanker DiseaseMycoparasitismValsa ceratospermaApple valsa canker, caused by the fungus Valsa ceratosperma, is one of the most destructive diseases of this crop. Conventional fungicide treatments are not effective enough; therefore, it is necessary to develop alternatives for the control of this pathogen. The use of Trichoderma strains as a biocontrol agent is a promising strategy for the environmentally friendly management of this disease. In this study, seven different isolates of Trichoderma spp. were obtained from the rhizospheric soil of healthy apple plants. The antagonistic capacity of the isolates against the pathogen V. ceratosperma was evaluated using the dual culture method and by the production of antibiotic non-volatile compounds. In the dual culture method, the interaction zone was observed by differential interference contrast microscopy and confocal laser scanning microscopy. The isolates RN-13, RN-33, and RN-34 inhibited more than 75% of mycelial growth, whereas RN-19, RN-16, and RN-15 inhibited growth by 65–72% with respect to the control. The mycoparasitic activity was evidenced by the coiling of hyphae around pathogen hyphae, appressorium-like structures, morphological deformations, and disintegration of mycelial walls. Apple shoots inoculated with Trichoderma isolates and then infected with V. ceratosperma showed the antagonist capacity of RN-16, RN-19, RN-15, and RN-18, which produced a significant decrease in the size of the lesion. Overall, the results showed the great potential of Trichoderma as a biocontrol agent against apple tree valsa canker. Further studies of this fungal interaction are required to design effective control strategies for the protection of apple tree orchards from this disease.Instituto de Patología VegetalFil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bernanrdi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Cazon, Luis Ignacio. Universidad Federal de Vicosa. Laboratorio de Epidemiología. Departamento de Fitopatología; BrasilFil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Ortega, Leandro Ismael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaSpringer2022-10-11T11:31:22Z2022-10-11T11:31:22Z2022-05-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13082https://link.springer.com/article/10.1007/s10658-022-02529-30929-18731573-8469 (online)https://doi.org/10.1007/s10658-022-02529-3European Journal of Plant Pathology 163 : 923–935 (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina.info:eu-repo/semantics/restrictedAccess2025-09-11T10:24:23Zoai:localhost:20.500.12123/13082instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:24.191INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
title Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
spellingShingle Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
Valetti, Lucio
Antagonism
Trichoderma
Apples
Biological Control Agents
Antagonismo
Valsa
Manzana
Agentes de Control Biológico
Biocontrol Agents
Canker Disease
Mycoparasitism
Valsa ceratosperma
title_short Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
title_full Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
title_fullStr Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
title_full_unstemmed Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
title_sort Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
dc.creator.none.fl_str_mv Valetti, Lucio
Bernanrdi Lima, Nelson
Cazon, Luis Ignacio
Crociara, Clara Sonia
Ortega, Leandro Ismael
Pastor, Silvina Estela
author Valetti, Lucio
author_facet Valetti, Lucio
Bernanrdi Lima, Nelson
Cazon, Luis Ignacio
Crociara, Clara Sonia
Ortega, Leandro Ismael
Pastor, Silvina Estela
author_role author
author2 Bernanrdi Lima, Nelson
Cazon, Luis Ignacio
Crociara, Clara Sonia
Ortega, Leandro Ismael
Pastor, Silvina Estela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Antagonism
Trichoderma
Apples
Biological Control Agents
Antagonismo
Valsa
Manzana
Agentes de Control Biológico
Biocontrol Agents
Canker Disease
Mycoparasitism
Valsa ceratosperma
topic Antagonism
Trichoderma
Apples
Biological Control Agents
Antagonismo
Valsa
Manzana
Agentes de Control Biológico
Biocontrol Agents
Canker Disease
Mycoparasitism
Valsa ceratosperma
dc.description.none.fl_txt_mv Apple valsa canker, caused by the fungus Valsa ceratosperma, is one of the most destructive diseases of this crop. Conventional fungicide treatments are not effective enough; therefore, it is necessary to develop alternatives for the control of this pathogen. The use of Trichoderma strains as a biocontrol agent is a promising strategy for the environmentally friendly management of this disease. In this study, seven different isolates of Trichoderma spp. were obtained from the rhizospheric soil of healthy apple plants. The antagonistic capacity of the isolates against the pathogen V. ceratosperma was evaluated using the dual culture method and by the production of antibiotic non-volatile compounds. In the dual culture method, the interaction zone was observed by differential interference contrast microscopy and confocal laser scanning microscopy. The isolates RN-13, RN-33, and RN-34 inhibited more than 75% of mycelial growth, whereas RN-19, RN-16, and RN-15 inhibited growth by 65–72% with respect to the control. The mycoparasitic activity was evidenced by the coiling of hyphae around pathogen hyphae, appressorium-like structures, morphological deformations, and disintegration of mycelial walls. Apple shoots inoculated with Trichoderma isolates and then infected with V. ceratosperma showed the antagonist capacity of RN-16, RN-19, RN-15, and RN-18, which produced a significant decrease in the size of the lesion. Overall, the results showed the great potential of Trichoderma as a biocontrol agent against apple tree valsa canker. Further studies of this fungal interaction are required to design effective control strategies for the protection of apple tree orchards from this disease.
Instituto de Patología Vegetal
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernanrdi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cazon, Luis Ignacio. Universidad Federal de Vicosa. Laboratorio de Epidemiología. Departamento de Fitopatología; Brasil
Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Ortega, Leandro Ismael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Apple valsa canker, caused by the fungus Valsa ceratosperma, is one of the most destructive diseases of this crop. Conventional fungicide treatments are not effective enough; therefore, it is necessary to develop alternatives for the control of this pathogen. The use of Trichoderma strains as a biocontrol agent is a promising strategy for the environmentally friendly management of this disease. In this study, seven different isolates of Trichoderma spp. were obtained from the rhizospheric soil of healthy apple plants. The antagonistic capacity of the isolates against the pathogen V. ceratosperma was evaluated using the dual culture method and by the production of antibiotic non-volatile compounds. In the dual culture method, the interaction zone was observed by differential interference contrast microscopy and confocal laser scanning microscopy. The isolates RN-13, RN-33, and RN-34 inhibited more than 75% of mycelial growth, whereas RN-19, RN-16, and RN-15 inhibited growth by 65–72% with respect to the control. The mycoparasitic activity was evidenced by the coiling of hyphae around pathogen hyphae, appressorium-like structures, morphological deformations, and disintegration of mycelial walls. Apple shoots inoculated with Trichoderma isolates and then infected with V. ceratosperma showed the antagonist capacity of RN-16, RN-19, RN-15, and RN-18, which produced a significant decrease in the size of the lesion. Overall, the results showed the great potential of Trichoderma as a biocontrol agent against apple tree valsa canker. Further studies of this fungal interaction are required to design effective control strategies for the protection of apple tree orchards from this disease.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-11T11:31:22Z
2022-10-11T11:31:22Z
2022-05-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13082
https://link.springer.com/article/10.1007/s10658-022-02529-3
0929-1873
1573-8469 (online)
https://doi.org/10.1007/s10658-022-02529-3
url http://hdl.handle.net/20.500.12123/13082
https://link.springer.com/article/10.1007/s10658-022-02529-3
https://doi.org/10.1007/s10658-022-02529-3
identifier_str_mv 0929-1873
1573-8469 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European Journal of Plant Pathology 163 : 923–935 (2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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