First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina

Autores
Piñeyro, Pablo Enrique; Lozada, Maria Ines; Alarcón, Laura Valeria; Sanguinetti, Ramon; Cappuccio, Javier Alejandro; Pérez, Estefanía Marisol; Vannucci, Fabio; Armocida, Alberto; Madson, Darin Michael; Perfumo, Carlos Juan; Quiroga, Maria Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. In order to determine if TGEV was circulating before the official report, a retrospective study on cases of neonatal diarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets with watery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identified during 2010–2015. Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. The feces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence of round particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoids consistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonal specific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situ hybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative for PEDV and rotavirus A. Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV in Argentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation.
EEA Marcos Juárez
Fil: Piñeyro, Pablo Enrique. Iowa State University. Veterinary Medicine. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Alarcón, Laura Valeria. HIPRA Argentina; Argentina
Fil: Sanguinetti, Ramon. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección de Laboratorios y Control Técnico; Argentina
Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.
Fil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Vannucci, Fabio. University of Minnesota. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Armocida, Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Madson, Darin Michael. Iowa State University. Veterinary Medicine. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fuente
BMC Veterinary Research 14 : 292 (2018)
Materia
Enfermedades de los Animales
Virus de los Animales
Cerdo
Gastroenteritis
Etiología
Lechón
Animal Diseases
Animal Viruses
Swine
Aetiology
Piglets
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3606
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3606
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in ArgentinaPiñeyro, Pablo EnriqueLozada, Maria InesAlarcón, Laura ValeriaSanguinetti, RamonCappuccio, Javier AlejandroPérez, Estefanía MarisolVannucci, FabioArmocida, AlbertoMadson, Darin MichaelPerfumo, Carlos JuanQuiroga, Maria AlejandraEnfermedades de los AnimalesVirus de los AnimalesCerdoGastroenteritisEtiologíaLechónAnimal DiseasesAnimal VirusesSwineAetiologyPigletsArgentinaBackground: In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. In order to determine if TGEV was circulating before the official report, a retrospective study on cases of neonatal diarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets with watery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identified during 2010–2015. Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. The feces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence of round particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoids consistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonal specific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situ hybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative for PEDV and rotavirus A. Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV in Argentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation.EEA Marcos JuárezFil: Piñeyro, Pablo Enrique. Iowa State University. Veterinary Medicine. Veterinary Diagnostic Laboratory; Estados UnidosFil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; ArgentinaFil: Alarcón, Laura Valeria. HIPRA Argentina; ArgentinaFil: Sanguinetti, Ramon. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección de Laboratorios y Control Técnico; ArgentinaFil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.Fil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; ArgentinaFil: Vannucci, Fabio. University of Minnesota. Veterinary Diagnostic Laboratory; Estados UnidosFil: Armocida, Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; ArgentinaFil: Madson, Darin Michael. Iowa State University. Veterinary Medicine. Veterinary Diagnostic Laboratory; Estados UnidosFil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina2018-10-17T11:53:13Z2018-10-17T11:53:13Z2018-09-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1615-9http://hdl.handle.net/20.500.12123/36061746-6148https://doi.org/10.1186/s12917-018-1615-9BMC Veterinary Research 14 : 292 (2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:37Zoai:localhost:20.500.12123/3606instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:37.941INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
spellingShingle First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
Piñeyro, Pablo Enrique
Enfermedades de los Animales
Virus de los Animales
Cerdo
Gastroenteritis
Etiología
Lechón
Animal Diseases
Animal Viruses
Swine
Aetiology
Piglets
Argentina
title_short First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_full First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_fullStr First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_full_unstemmed First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_sort First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
dc.creator.none.fl_str_mv Piñeyro, Pablo Enrique
Lozada, Maria Ines
Alarcón, Laura Valeria
Sanguinetti, Ramon
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio
Armocida, Alberto
Madson, Darin Michael
Perfumo, Carlos Juan
Quiroga, Maria Alejandra
author Piñeyro, Pablo Enrique
author_facet Piñeyro, Pablo Enrique
Lozada, Maria Ines
Alarcón, Laura Valeria
Sanguinetti, Ramon
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio
Armocida, Alberto
Madson, Darin Michael
Perfumo, Carlos Juan
Quiroga, Maria Alejandra
author_role author
author2 Lozada, Maria Ines
Alarcón, Laura Valeria
Sanguinetti, Ramon
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio
Armocida, Alberto
Madson, Darin Michael
Perfumo, Carlos Juan
Quiroga, Maria Alejandra
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Virus de los Animales
Cerdo
Gastroenteritis
Etiología
Lechón
Animal Diseases
Animal Viruses
Swine
Aetiology
Piglets
Argentina
topic Enfermedades de los Animales
Virus de los Animales
Cerdo
Gastroenteritis
Etiología
Lechón
Animal Diseases
Animal Viruses
Swine
Aetiology
Piglets
Argentina
dc.description.none.fl_txt_mv Background: In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. In order to determine if TGEV was circulating before the official report, a retrospective study on cases of neonatal diarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets with watery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identified during 2010–2015. Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. The feces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence of round particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoids consistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonal specific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situ hybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative for PEDV and rotavirus A. Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV in Argentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation.
EEA Marcos Juárez
Fil: Piñeyro, Pablo Enrique. Iowa State University. Veterinary Medicine. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Alarcón, Laura Valeria. HIPRA Argentina; Argentina
Fil: Sanguinetti, Ramon. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección de Laboratorios y Control Técnico; Argentina
Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.
Fil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Vannucci, Fabio. University of Minnesota. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Armocida, Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Madson, Darin Michael. Iowa State University. Veterinary Medicine. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria; Argentina
description Background: In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. In order to determine if TGEV was circulating before the official report, a retrospective study on cases of neonatal diarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets with watery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identified during 2010–2015. Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. The feces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence of round particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoids consistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonal specific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situ hybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative for PEDV and rotavirus A. Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV in Argentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-17T11:53:13Z
2018-10-17T11:53:13Z
2018-09-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1615-9
http://hdl.handle.net/20.500.12123/3606
1746-6148
https://doi.org/10.1186/s12917-018-1615-9
url https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1615-9
http://hdl.handle.net/20.500.12123/3606
https://doi.org/10.1186/s12917-018-1615-9
identifier_str_mv 1746-6148
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv BMC Veterinary Research 14 : 292 (2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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