First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina

Autores
Piñeyro Piñeiro, Pablo Enrique; Lozada, María Inés; Alarcon, Laura Valeria; Sanguinetti, Ramon; Cappuccio, Javier Alejandro; Pérez, Estefanía Marisol; Vannucci, Fabio; Armocida, Alberto Domingo; Madson, Darin Michael; Perfumo, Carlos Juan; Quiroga, María Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: (TGEV) was made by the NationalServices of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notificationwas based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. Inorder to determine if TGEV was circulating before the official report, a retrospective study on cases of neonataldiarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets withwatery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identifiedduring 2010?2015.Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. Thefeces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence ofround particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoidsconsistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonalspecific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situhybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative forPEDV and rotavirus A.Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV inArgentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiologicaldistribution and coinfection with other enteric pathogens that mask the clinical presentation.
Fil: Piñeyro Piñeiro, Pablo Enrique. University Of Iowa. Ames Laboratory; Estados Unidos. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alarcon, Laura Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Sanguinetti, Ramon. Ministerio de Agricultura, Ganadería, Pesca y Alimento; Argentina
Fil: Cappuccio, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vannucci, Fabio. University Of Minnesota. School Of Public Health.; Estados Unidos
Fil: Armocida, Alberto Domingo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Madson, Darin Michael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
Fil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
Materia
DIARRHEA
MORTALITY
PIGLETS
PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150565
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in ArgentinaPiñeyro Piñeiro, Pablo EnriqueLozada, María InésAlarcon, Laura ValeriaSanguinetti, RamonCappuccio, Javier AlejandroPérez, Estefanía MarisolVannucci, FabioArmocida, Alberto DomingoMadson, Darin MichaelPerfumo, Carlos JuanQuiroga, María AlejandraDIARRHEAMORTALITYPIGLETSPORCINE TRANSMISSIBLE GASTROENTERITIS VIRUShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: (TGEV) was made by the NationalServices of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notificationwas based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. Inorder to determine if TGEV was circulating before the official report, a retrospective study on cases of neonataldiarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets withwatery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identifiedduring 2010?2015.Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. Thefeces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence ofround particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoidsconsistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonalspecific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situhybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative forPEDV and rotavirus A.Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV inArgentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiologicaldistribution and coinfection with other enteric pathogens that mask the clinical presentation.Fil: Piñeyro Piñeiro, Pablo Enrique. University Of Iowa. Ames Laboratory; Estados Unidos. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alarcon, Laura Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Sanguinetti, Ramon. Ministerio de Agricultura, Ganadería, Pesca y Alimento; ArgentinaFil: Cappuccio, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vannucci, Fabio. University Of Minnesota. School Of Public Health.; Estados UnidosFil: Armocida, Alberto Domingo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Madson, Darin Michael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; ArgentinaFil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; ArgentinaBioMed Central2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150565Piñeyro Piñeiro, Pablo Enrique; Lozada, María Inés; Alarcon, Laura Valeria; Sanguinetti, Ramon; Cappuccio, Javier Alejandro; et al.; First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina; BioMed Central; BMC Veterinary Research; 14; 1; 9-2018; ´1-81746-6148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1615-9info:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-018-1615-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:10Zoai:ri.conicet.gov.ar:11336/150565instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:10.524CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
spellingShingle First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
Piñeyro Piñeiro, Pablo Enrique
DIARRHEA
MORTALITY
PIGLETS
PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS
title_short First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_full First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_fullStr First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_full_unstemmed First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_sort First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
dc.creator.none.fl_str_mv Piñeyro Piñeiro, Pablo Enrique
Lozada, María Inés
Alarcon, Laura Valeria
Sanguinetti, Ramon
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio
Armocida, Alberto Domingo
Madson, Darin Michael
Perfumo, Carlos Juan
Quiroga, María Alejandra
author Piñeyro Piñeiro, Pablo Enrique
author_facet Piñeyro Piñeiro, Pablo Enrique
Lozada, María Inés
Alarcon, Laura Valeria
Sanguinetti, Ramon
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio
Armocida, Alberto Domingo
Madson, Darin Michael
Perfumo, Carlos Juan
Quiroga, María Alejandra
author_role author
author2 Lozada, María Inés
Alarcon, Laura Valeria
Sanguinetti, Ramon
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio
Armocida, Alberto Domingo
Madson, Darin Michael
Perfumo, Carlos Juan
Quiroga, María Alejandra
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIARRHEA
MORTALITY
PIGLETS
PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS
topic DIARRHEA
MORTALITY
PIGLETS
PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: (TGEV) was made by the NationalServices of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notificationwas based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. Inorder to determine if TGEV was circulating before the official report, a retrospective study on cases of neonataldiarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets withwatery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identifiedduring 2010?2015.Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. Thefeces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence ofround particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoidsconsistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonalspecific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situhybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative forPEDV and rotavirus A.Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV inArgentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiologicaldistribution and coinfection with other enteric pathogens that mask the clinical presentation.
Fil: Piñeyro Piñeiro, Pablo Enrique. University Of Iowa. Ames Laboratory; Estados Unidos. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozada, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alarcon, Laura Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Sanguinetti, Ramon. Ministerio de Agricultura, Ganadería, Pesca y Alimento; Argentina
Fil: Cappuccio, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Pérez, Estefanía Marisol. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vannucci, Fabio. University Of Minnesota. School Of Public Health.; Estados Unidos
Fil: Armocida, Alberto Domingo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Madson, Darin Michael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
Fil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
description Background: (TGEV) was made by the NationalServices of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notificationwas based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. Inorder to determine if TGEV was circulating before the official report, a retrospective study on cases of neonataldiarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets withwatery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identifiedduring 2010?2015.Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. Thefeces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence ofround particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoidsconsistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonalspecific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situhybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative forPEDV and rotavirus A.Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV inArgentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiologicaldistribution and coinfection with other enteric pathogens that mask the clinical presentation.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150565
Piñeyro Piñeiro, Pablo Enrique; Lozada, María Inés; Alarcon, Laura Valeria; Sanguinetti, Ramon; Cappuccio, Javier Alejandro; et al.; First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina; BioMed Central; BMC Veterinary Research; 14; 1; 9-2018; ´1-8
1746-6148
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150565
identifier_str_mv Piñeyro Piñeiro, Pablo Enrique; Lozada, María Inés; Alarcon, Laura Valeria; Sanguinetti, Ramon; Cappuccio, Javier Alejandro; et al.; First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina; BioMed Central; BMC Veterinary Research; 14; 1; 9-2018; ´1-8
1746-6148
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1615-9
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-018-1615-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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