Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance

Autores
Brugnoli, Elsa Andrea; Martinez, Eric Javier; Ferrari Usandizaga, Silvana Consuelo; Zili, Alex Leonel; Urbani, Mario; Acuña, Carlos Alberto
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids.
EEA Corrientes
Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Brugnoli, Elsa Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Martínez, Eric Javier. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Ferrari Usandizaga, Silvana Consuelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; Argentina
Fil: Zili, Alex Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Zili, Alex Leonel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Urbani, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Urbani, Mario. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Acuña, Carlos Alberto. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fuente
Crop Science 59 (4) : 1617-1624 (July-August 2019)
Materia
Gramíneas
Mejoramiento Genético
Híbridos
Genotipos
Apomixis
Poliploidia
Grasses
Genetic Improvement
Hybrids
Genotypes
Paspalum
Polyploidy
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15154
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15154
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid PerformanceBrugnoli, Elsa AndreaMartinez, Eric JavierFerrari Usandizaga, Silvana ConsueloZili, Alex LeonelUrbani, MarioAcuña, Carlos AlbertoGramíneasMejoramiento GenéticoHíbridosGenotiposApomixisPoliploidiaGrassesGenetic ImprovementHybridsGenotypesPaspalumPolyploidyApomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids.EEA CorrientesFil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: Brugnoli, Elsa Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: Martínez, Eric Javier. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Ferrari Usandizaga, Silvana Consuelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; ArgentinaFil: Zili, Alex Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: Zili, Alex Leonel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Urbani, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: Urbani, Mario. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: Acuña, Carlos Alberto. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaWiley2023-09-08T14:31:27Z2023-09-08T14:31:27Z2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15154https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2018.12.07710011-183Xhttps://doi.org/10.2135/cropsci2018.12.0771Crop Science 59 (4) : 1617-1624 (July-August 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:27Zoai:localhost:20.500.12123/15154instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:27.467INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
title Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
spellingShingle Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
Brugnoli, Elsa Andrea
Gramíneas
Mejoramiento Genético
Híbridos
Genotipos
Apomixis
Poliploidia
Grasses
Genetic Improvement
Hybrids
Genotypes
Paspalum
Polyploidy
title_short Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
title_full Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
title_fullStr Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
title_full_unstemmed Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
title_sort Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
dc.creator.none.fl_str_mv Brugnoli, Elsa Andrea
Martinez, Eric Javier
Ferrari Usandizaga, Silvana Consuelo
Zili, Alex Leonel
Urbani, Mario
Acuña, Carlos Alberto
author Brugnoli, Elsa Andrea
author_facet Brugnoli, Elsa Andrea
Martinez, Eric Javier
Ferrari Usandizaga, Silvana Consuelo
Zili, Alex Leonel
Urbani, Mario
Acuña, Carlos Alberto
author_role author
author2 Martinez, Eric Javier
Ferrari Usandizaga, Silvana Consuelo
Zili, Alex Leonel
Urbani, Mario
Acuña, Carlos Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Gramíneas
Mejoramiento Genético
Híbridos
Genotipos
Apomixis
Poliploidia
Grasses
Genetic Improvement
Hybrids
Genotypes
Paspalum
Polyploidy
topic Gramíneas
Mejoramiento Genético
Híbridos
Genotipos
Apomixis
Poliploidia
Grasses
Genetic Improvement
Hybrids
Genotypes
Paspalum
Polyploidy
dc.description.none.fl_txt_mv Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids.
EEA Corrientes
Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Brugnoli, Elsa Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Martínez, Eric Javier. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Ferrari Usandizaga, Silvana Consuelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; Argentina
Fil: Zili, Alex Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Zili, Alex Leonel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Urbani, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Urbani, Mario. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Acuña, Carlos Alberto. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids.
publishDate 2019
dc.date.none.fl_str_mv 2019
2023-09-08T14:31:27Z
2023-09-08T14:31:27Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15154
https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2018.12.0771
0011-183X
https://doi.org/10.2135/cropsci2018.12.0771
url http://hdl.handle.net/20.500.12123/15154
https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2018.12.0771
https://doi.org/10.2135/cropsci2018.12.0771
identifier_str_mv 0011-183X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Crop Science 59 (4) : 1617-1624 (July-August 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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