Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population

Autores
Zilli, Alex Leonel; Acuña, Carlos Alberto; Schulz, R. R.; Brugnoli, Elsa Andrea; Guidalevich, Veronica; Quarin, Camilo Luis; Martínez, Eric Javier
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The improvement of bahiagrass, Paspalum notatum Flüggé, has been inhibited by reduced levels of genetic diversity in sexual tetraploid germplasm. A few experimental sexual tetraploid genotypes (ESTGs) have been generated by chromosome doubling, but these plants typically exhibit low vigor. The objectives of this work were to generate and characterize the ploidy level, mode of reproduction, and fertility of a novel 308 individual sexual synthetic tetraploid population (SSTP) developed by intercrossing 29 sexual F1 hybrids originated by hybridizing several naturally occurring apomictic tetraploids from diverse origin with a few ESTGs. Ploidy levels were determined using flow cytometry, and reproductive modes were evaluated by a molecular assay with apospory-linked markers and embryo sac observations. The tetraploid level and the sexual mode of reproduction remained stable after two cycles of recombination during the generation of the SSTP. Fertility was evaluated based on seed set under self- and open pollination during 3 yr. The SSTP exhibited in average 30.2 and 15.2% seed set under open and self-pollina-tion, respectively, showing a predominantly cross-pollination behavior with variable levels of self-fertility. There were no differences in terms of fertility between the SSTP and the ESTG. The novel tetraploid population behaves as sexual and cross-pollinated, and it is expected to allow a more efficient genetic improvement under the proposed breeding approaches.
Fil: Zilli, Alex Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Schulz, R. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Guidalevich, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Materia
PASPALUM
APOMIXIS
POLIPLOIDÍA
FERTILIDAD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89695
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid populationZilli, Alex LeonelAcuña, Carlos AlbertoSchulz, R. R.Brugnoli, Elsa AndreaGuidalevich, VeronicaQuarin, Camilo LuisMartínez, Eric JavierPASPALUMAPOMIXISPOLIPLOIDÍAFERTILIDADhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The improvement of bahiagrass, Paspalum notatum Flüggé, has been inhibited by reduced levels of genetic diversity in sexual tetraploid germplasm. A few experimental sexual tetraploid genotypes (ESTGs) have been generated by chromosome doubling, but these plants typically exhibit low vigor. The objectives of this work were to generate and characterize the ploidy level, mode of reproduction, and fertility of a novel 308 individual sexual synthetic tetraploid population (SSTP) developed by intercrossing 29 sexual F1 hybrids originated by hybridizing several naturally occurring apomictic tetraploids from diverse origin with a few ESTGs. Ploidy levels were determined using flow cytometry, and reproductive modes were evaluated by a molecular assay with apospory-linked markers and embryo sac observations. The tetraploid level and the sexual mode of reproduction remained stable after two cycles of recombination during the generation of the SSTP. Fertility was evaluated based on seed set under self- and open pollination during 3 yr. The SSTP exhibited in average 30.2 and 15.2% seed set under open and self-pollina-tion, respectively, showing a predominantly cross-pollination behavior with variable levels of self-fertility. There were no differences in terms of fertility between the SSTP and the ESTG. The novel tetraploid population behaves as sexual and cross-pollinated, and it is expected to allow a more efficient genetic improvement under the proposed breeding approaches.Fil: Zilli, Alex Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Schulz, R. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Guidalevich, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaCrop Science Society of America2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89695Zilli, Alex Leonel; Acuña, Carlos Alberto; Schulz, R. R.; Brugnoli, Elsa Andrea; Guidalevich, Veronica; et al.; Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population; Crop Science Society of America; Crop Science; 58; 2; 3-2018; 762-7720011-183XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2017.07.0457info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/58/2/762info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:43:48Zoai:ri.conicet.gov.ar:11336/89695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:43:48.418CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
title Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
spellingShingle Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
Zilli, Alex Leonel
PASPALUM
APOMIXIS
POLIPLOIDÍA
FERTILIDAD
title_short Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
title_full Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
title_fullStr Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
title_full_unstemmed Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
title_sort Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population
dc.creator.none.fl_str_mv Zilli, Alex Leonel
Acuña, Carlos Alberto
Schulz, R. R.
Brugnoli, Elsa Andrea
Guidalevich, Veronica
Quarin, Camilo Luis
Martínez, Eric Javier
author Zilli, Alex Leonel
author_facet Zilli, Alex Leonel
Acuña, Carlos Alberto
Schulz, R. R.
Brugnoli, Elsa Andrea
Guidalevich, Veronica
Quarin, Camilo Luis
Martínez, Eric Javier
author_role author
author2 Acuña, Carlos Alberto
Schulz, R. R.
Brugnoli, Elsa Andrea
Guidalevich, Veronica
Quarin, Camilo Luis
Martínez, Eric Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PASPALUM
APOMIXIS
POLIPLOIDÍA
FERTILIDAD
topic PASPALUM
APOMIXIS
POLIPLOIDÍA
FERTILIDAD
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The improvement of bahiagrass, Paspalum notatum Flüggé, has been inhibited by reduced levels of genetic diversity in sexual tetraploid germplasm. A few experimental sexual tetraploid genotypes (ESTGs) have been generated by chromosome doubling, but these plants typically exhibit low vigor. The objectives of this work were to generate and characterize the ploidy level, mode of reproduction, and fertility of a novel 308 individual sexual synthetic tetraploid population (SSTP) developed by intercrossing 29 sexual F1 hybrids originated by hybridizing several naturally occurring apomictic tetraploids from diverse origin with a few ESTGs. Ploidy levels were determined using flow cytometry, and reproductive modes were evaluated by a molecular assay with apospory-linked markers and embryo sac observations. The tetraploid level and the sexual mode of reproduction remained stable after two cycles of recombination during the generation of the SSTP. Fertility was evaluated based on seed set under self- and open pollination during 3 yr. The SSTP exhibited in average 30.2 and 15.2% seed set under open and self-pollina-tion, respectively, showing a predominantly cross-pollination behavior with variable levels of self-fertility. There were no differences in terms of fertility between the SSTP and the ESTG. The novel tetraploid population behaves as sexual and cross-pollinated, and it is expected to allow a more efficient genetic improvement under the proposed breeding approaches.
Fil: Zilli, Alex Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Schulz, R. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Guidalevich, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description The improvement of bahiagrass, Paspalum notatum Flüggé, has been inhibited by reduced levels of genetic diversity in sexual tetraploid germplasm. A few experimental sexual tetraploid genotypes (ESTGs) have been generated by chromosome doubling, but these plants typically exhibit low vigor. The objectives of this work were to generate and characterize the ploidy level, mode of reproduction, and fertility of a novel 308 individual sexual synthetic tetraploid population (SSTP) developed by intercrossing 29 sexual F1 hybrids originated by hybridizing several naturally occurring apomictic tetraploids from diverse origin with a few ESTGs. Ploidy levels were determined using flow cytometry, and reproductive modes were evaluated by a molecular assay with apospory-linked markers and embryo sac observations. The tetraploid level and the sexual mode of reproduction remained stable after two cycles of recombination during the generation of the SSTP. Fertility was evaluated based on seed set under self- and open pollination during 3 yr. The SSTP exhibited in average 30.2 and 15.2% seed set under open and self-pollina-tion, respectively, showing a predominantly cross-pollination behavior with variable levels of self-fertility. There were no differences in terms of fertility between the SSTP and the ESTG. The novel tetraploid population behaves as sexual and cross-pollinated, and it is expected to allow a more efficient genetic improvement under the proposed breeding approaches.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89695
Zilli, Alex Leonel; Acuña, Carlos Alberto; Schulz, R. R.; Brugnoli, Elsa Andrea; Guidalevich, Veronica; et al.; Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population; Crop Science Society of America; Crop Science; 58; 2; 3-2018; 762-772
0011-183X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89695
identifier_str_mv Zilli, Alex Leonel; Acuña, Carlos Alberto; Schulz, R. R.; Brugnoli, Elsa Andrea; Guidalevich, Veronica; et al.; Widening the gene pool of sexual tetraploid bahiagrass: Generation and reproductive characterization of a sexual synthetic tetraploid population; Crop Science Society of America; Crop Science; 58; 2; 3-2018; 762-772
0011-183X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2017.07.0457
info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/58/2/762
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Crop Science Society of America
publisher.none.fl_str_mv Crop Science Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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