Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers

Autores
Soriano Perez, María Laura; Funes, Javier Alejandro; Flores Bracamonte, Carolina; Ibarra, Luis Exequiel; Forrellad, Marina Andrea; Taboga, Oscar Alberto; Cariddi, Laura Noelia; Salinas, Facundo José; Ortega, Hugo Héctor; Alustiza, Fabrisio Eduardo; Molina, Maria
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
“Smart” nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate pNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of pNIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into pNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that pNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers.
EEA Marcos Juárez
Fil: Soriano Perez, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Funes, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Flores Bracamonte, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina
Fil: Flores Bracamonte, Carolina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Forrellad, Marina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Salinas, Facundo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salinas, Facundo José. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Molina, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Molina, Maria. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fuente
International Journal of Pharmaceutics 630 : 122435 (January 2023)
Materia
Nanotecnología
Nanomateriales
Vacuna
Macrofagos
Nanotechnology
Nanomaterials
Vaccines
Macrophages
Nanogeles
Nanogels
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13655
Acceder
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spelling Development and biological evaluation of pNIPAM-based nanogels as vaccine carriersSoriano Perez, María LauraFunes, Javier AlejandroFlores Bracamonte, CarolinaIbarra, Luis ExequielForrellad, Marina AndreaTaboga, Oscar AlbertoCariddi, Laura NoeliaSalinas, Facundo JoséOrtega, Hugo HéctorAlustiza, Fabrisio EduardoMolina, MariaNanotecnologíaNanomaterialesVacunaMacrofagosNanotechnologyNanomaterialsVaccinesMacrophagesNanogelesNanogels“Smart” nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate pNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of pNIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into pNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that pNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers.EEA Marcos JuárezFil: Soriano Perez, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Funes, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Flores Bracamonte, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; ArgentinaFil: Flores Bracamonte, Carolina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; ArgentinaFil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Forrellad, Marina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Salinas, Facundo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Salinas, Facundo José. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Ortega, Hugo Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Molina, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Molina, Maria. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2023-12-192022-12-19T17:28:17Z2022-12-19T17:28:17Z2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13655https://www.sciencedirect.com/science/article/abs/pii/S03785173220099050378-51731873-3476https://doi.org/10.1016/j.ijpharm.2022.122435International Journal of Pharmaceutics 630 : 122435 (January 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud públicainfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:40Zoai:localhost:20.500.12123/13655instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:40.789INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
title Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
spellingShingle Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
Soriano Perez, María Laura
Nanotecnología
Nanomateriales
Vacuna
Macrofagos
Nanotechnology
Nanomaterials
Vaccines
Macrophages
Nanogeles
Nanogels
title_short Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
title_full Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
title_fullStr Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
title_full_unstemmed Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
title_sort Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers
dc.creator.none.fl_str_mv Soriano Perez, María Laura
Funes, Javier Alejandro
Flores Bracamonte, Carolina
Ibarra, Luis Exequiel
Forrellad, Marina Andrea
Taboga, Oscar Alberto
Cariddi, Laura Noelia
Salinas, Facundo José
Ortega, Hugo Héctor
Alustiza, Fabrisio Eduardo
Molina, Maria
author Soriano Perez, María Laura
author_facet Soriano Perez, María Laura
Funes, Javier Alejandro
Flores Bracamonte, Carolina
Ibarra, Luis Exequiel
Forrellad, Marina Andrea
Taboga, Oscar Alberto
Cariddi, Laura Noelia
Salinas, Facundo José
Ortega, Hugo Héctor
Alustiza, Fabrisio Eduardo
Molina, Maria
author_role author
author2 Funes, Javier Alejandro
Flores Bracamonte, Carolina
Ibarra, Luis Exequiel
Forrellad, Marina Andrea
Taboga, Oscar Alberto
Cariddi, Laura Noelia
Salinas, Facundo José
Ortega, Hugo Héctor
Alustiza, Fabrisio Eduardo
Molina, Maria
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanotecnología
Nanomateriales
Vacuna
Macrofagos
Nanotechnology
Nanomaterials
Vaccines
Macrophages
Nanogeles
Nanogels
topic Nanotecnología
Nanomateriales
Vacuna
Macrofagos
Nanotechnology
Nanomaterials
Vaccines
Macrophages
Nanogeles
Nanogels
dc.description.none.fl_txt_mv “Smart” nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate pNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of pNIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into pNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that pNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers.
EEA Marcos Juárez
Fil: Soriano Perez, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Funes, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Flores Bracamonte, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina
Fil: Flores Bracamonte, Carolina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Forrellad, Marina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Salinas, Facundo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salinas, Facundo José. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Molina, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Molina, Maria. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
description “Smart” nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate pNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of pNIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into pNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that pNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-19T17:28:17Z
2022-12-19T17:28:17Z
2023-01
info:eu-repo/date/embargoEnd/2023-12-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13655
https://www.sciencedirect.com/science/article/abs/pii/S0378517322009905
0378-5173
1873-3476
https://doi.org/10.1016/j.ijpharm.2022.122435
url http://hdl.handle.net/20.500.12123/13655
https://www.sciencedirect.com/science/article/abs/pii/S0378517322009905
https://doi.org/10.1016/j.ijpharm.2022.122435
identifier_str_mv 0378-5173
1873-3476
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv International Journal of Pharmaceutics 630 : 122435 (January 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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