A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples

Autores
Okada, Elena; Coggan, Timothy; Anumol, Tarun; Clarke, Bradley; Allison, Graeme
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glyphosate is currently the most widely used herbicide in the world, yet screening of environmental waters for this chemical is limited by the need for specialized derivatization and measurement methods that can be tedious and time-consuming. In this work, we present a novel method for the detection and quantification at trace levels of glyphosate and aminomethylphosphonic acid (AMPA) in environmental water samples. The detection and quantification of the analytes was performed by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). Chromatographic separation was achieved with an ion-exchange column and a pH-gradient elution of a solution of ammonium hydroxide and ammonium acetate. The limit of detection for glyphosate and AMPA was 0.25 μg L-1 and the limit of quantification was 0.5 μg L-1with a 20-μL injection. The method was used to investigate the levels of glyphosate and AMPA in surface water samples from the Yarra River catchment area and urban constructed stormwater wetlands. The results indicate that at the time of sampling, no glyphosate or AMPA was present in the samples from the Yarra River catchment area (n = 10). However, glyphosate was detected above the limit of quantification in 33% of the wetland samples (n = 12), with concentrations ranging from 1.95 to 2.96 μg L-1. Similarly, AMPA was quantified in 83% of the wetland samples, with concentrations ranging from 0.55 to 2.42 μg L-1. To our knowledge, this is the first report of a pH-gradient LC–MS/MS method for glyphosate and AMPA analysis at ultratrace levels, with minimal sample processing, avoiding costly, time-consuming derivatization and preconcentration steps.
EEA Balcarce
Fil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Coggan, Timothy. RMIT University. School of Science; Australia.
Fil: Anumol, Tarun. Agilent Technologies Inc; Estados Unidos.
Fil: Clarke, Bradley. RMIT University. School of Science; Australia.
Fil: Allison, Graeme. RMIT University. School of Science; Australia.
Fuente
Analytical and Bioanalytical Chemistry 411 : 715–724 (2019)
Materia
Glifosato
Inyección
Métodos de Aplicación
Intercambio Iónico
Agua Superficial
Glyphosate
Injection
Application Methods
Ion Exchange
Surface Water
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8157
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spelling A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samplesOkada, ElenaCoggan, TimothyAnumol, TarunClarke, BradleyAllison, GraemeGlifosatoInyecciónMétodos de AplicaciónIntercambio IónicoAgua SuperficialGlyphosateInjectionApplication MethodsIon ExchangeSurface WaterGlyphosate is currently the most widely used herbicide in the world, yet screening of environmental waters for this chemical is limited by the need for specialized derivatization and measurement methods that can be tedious and time-consuming. In this work, we present a novel method for the detection and quantification at trace levels of glyphosate and aminomethylphosphonic acid (AMPA) in environmental water samples. The detection and quantification of the analytes was performed by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). Chromatographic separation was achieved with an ion-exchange column and a pH-gradient elution of a solution of ammonium hydroxide and ammonium acetate. The limit of detection for glyphosate and AMPA was 0.25 μg L-1 and the limit of quantification was 0.5 μg L-1with a 20-μL injection. The method was used to investigate the levels of glyphosate and AMPA in surface water samples from the Yarra River catchment area and urban constructed stormwater wetlands. The results indicate that at the time of sampling, no glyphosate or AMPA was present in the samples from the Yarra River catchment area (n = 10). However, glyphosate was detected above the limit of quantification in 33% of the wetland samples (n = 12), with concentrations ranging from 1.95 to 2.96 μg L-1. Similarly, AMPA was quantified in 83% of the wetland samples, with concentrations ranging from 0.55 to 2.42 μg L-1. To our knowledge, this is the first report of a pH-gradient LC–MS/MS method for glyphosate and AMPA analysis at ultratrace levels, with minimal sample processing, avoiding costly, time-consuming derivatization and preconcentration steps.EEA BalcarceFil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Coggan, Timothy. RMIT University. School of Science; Australia.Fil: Anumol, Tarun. Agilent Technologies Inc; Estados Unidos.Fil: Clarke, Bradley. RMIT University. School of Science; Australia.Fil: Allison, Graeme. RMIT University. School of Science; Australia.Springer Verlag2020-10-30T11:16:04Z2020-10-30T11:16:04Z2018-12-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8157https://link.springer.com/article/10.1007/s00216-018-1490-z1618-2642https://doi.org/10.1007/s00216-018-1490-zAnalytical and Bioanalytical Chemistry 411 : 715–724 (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:40Zoai:localhost:20.500.12123/8157instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:40.836INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
title A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
spellingShingle A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
Okada, Elena
Glifosato
Inyección
Métodos de Aplicación
Intercambio Iónico
Agua Superficial
Glyphosate
Injection
Application Methods
Ion Exchange
Surface Water
title_short A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
title_full A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
title_fullStr A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
title_full_unstemmed A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
title_sort A simple and rapid direct injection method for the determination of glyphosate and AMPA in environmental water samples
dc.creator.none.fl_str_mv Okada, Elena
Coggan, Timothy
Anumol, Tarun
Clarke, Bradley
Allison, Graeme
author Okada, Elena
author_facet Okada, Elena
Coggan, Timothy
Anumol, Tarun
Clarke, Bradley
Allison, Graeme
author_role author
author2 Coggan, Timothy
Anumol, Tarun
Clarke, Bradley
Allison, Graeme
author2_role author
author
author
author
dc.subject.none.fl_str_mv Glifosato
Inyección
Métodos de Aplicación
Intercambio Iónico
Agua Superficial
Glyphosate
Injection
Application Methods
Ion Exchange
Surface Water
topic Glifosato
Inyección
Métodos de Aplicación
Intercambio Iónico
Agua Superficial
Glyphosate
Injection
Application Methods
Ion Exchange
Surface Water
dc.description.none.fl_txt_mv Glyphosate is currently the most widely used herbicide in the world, yet screening of environmental waters for this chemical is limited by the need for specialized derivatization and measurement methods that can be tedious and time-consuming. In this work, we present a novel method for the detection and quantification at trace levels of glyphosate and aminomethylphosphonic acid (AMPA) in environmental water samples. The detection and quantification of the analytes was performed by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). Chromatographic separation was achieved with an ion-exchange column and a pH-gradient elution of a solution of ammonium hydroxide and ammonium acetate. The limit of detection for glyphosate and AMPA was 0.25 μg L-1 and the limit of quantification was 0.5 μg L-1with a 20-μL injection. The method was used to investigate the levels of glyphosate and AMPA in surface water samples from the Yarra River catchment area and urban constructed stormwater wetlands. The results indicate that at the time of sampling, no glyphosate or AMPA was present in the samples from the Yarra River catchment area (n = 10). However, glyphosate was detected above the limit of quantification in 33% of the wetland samples (n = 12), with concentrations ranging from 1.95 to 2.96 μg L-1. Similarly, AMPA was quantified in 83% of the wetland samples, with concentrations ranging from 0.55 to 2.42 μg L-1. To our knowledge, this is the first report of a pH-gradient LC–MS/MS method for glyphosate and AMPA analysis at ultratrace levels, with minimal sample processing, avoiding costly, time-consuming derivatization and preconcentration steps.
EEA Balcarce
Fil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Coggan, Timothy. RMIT University. School of Science; Australia.
Fil: Anumol, Tarun. Agilent Technologies Inc; Estados Unidos.
Fil: Clarke, Bradley. RMIT University. School of Science; Australia.
Fil: Allison, Graeme. RMIT University. School of Science; Australia.
description Glyphosate is currently the most widely used herbicide in the world, yet screening of environmental waters for this chemical is limited by the need for specialized derivatization and measurement methods that can be tedious and time-consuming. In this work, we present a novel method for the detection and quantification at trace levels of glyphosate and aminomethylphosphonic acid (AMPA) in environmental water samples. The detection and quantification of the analytes was performed by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). Chromatographic separation was achieved with an ion-exchange column and a pH-gradient elution of a solution of ammonium hydroxide and ammonium acetate. The limit of detection for glyphosate and AMPA was 0.25 μg L-1 and the limit of quantification was 0.5 μg L-1with a 20-μL injection. The method was used to investigate the levels of glyphosate and AMPA in surface water samples from the Yarra River catchment area and urban constructed stormwater wetlands. The results indicate that at the time of sampling, no glyphosate or AMPA was present in the samples from the Yarra River catchment area (n = 10). However, glyphosate was detected above the limit of quantification in 33% of the wetland samples (n = 12), with concentrations ranging from 1.95 to 2.96 μg L-1. Similarly, AMPA was quantified in 83% of the wetland samples, with concentrations ranging from 0.55 to 2.42 μg L-1. To our knowledge, this is the first report of a pH-gradient LC–MS/MS method for glyphosate and AMPA analysis at ultratrace levels, with minimal sample processing, avoiding costly, time-consuming derivatization and preconcentration steps.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-10
2020-10-30T11:16:04Z
2020-10-30T11:16:04Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8157
https://link.springer.com/article/10.1007/s00216-018-1490-z
1618-2642
https://doi.org/10.1007/s00216-018-1490-z
url http://hdl.handle.net/20.500.12123/8157
https://link.springer.com/article/10.1007/s00216-018-1490-z
https://doi.org/10.1007/s00216-018-1490-z
identifier_str_mv 1618-2642
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Analytical and Bioanalytical Chemistry 411 : 715–724 (2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341382300106752
score 12.623145

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