Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method

Autores
Demonte, Luisina Delma; Michlig, Nicolás; Gaggiotti, Monica Del Carmen; Adam, Claudia Guadalupe; Beldoménico, Horacio Ramón; Repetti, María Rosa
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at EEA Rafaela
Fil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Adam, Claudia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. IQAL; Argentina
Fil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fuente
Science of The Total Environment 645 : 34-43 (December 2018) In Progress
Materia
Granjas Lecheras
Agua
Glifosato
Glufosinato
Experimentación
Dairy Farms
Water
Glyphosate
Glufosinate
Experimentation
Argentina
Tambos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2915
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2915
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spelling Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS methodDemonte, Luisina DelmaMichlig, NicolásGaggiotti, Monica Del CarmenAdam, Claudia GuadalupeBeldoménico, Horacio RamónRepetti, María RosaGranjas LecherasAguaGlifosatoGlufosinatoExperimentaciónDairy FarmsWaterGlyphosateGlufosinateExperimentationArgentinaTambosArgentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes.EEA RafaelaFil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); ArgentinaFil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); ArgentinaFil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Adam, Claudia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. IQAL; ArgentinaFil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina2018-07-30T14:16:12Z2018-07-30T14:16:12Z2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0048969718324161http://hdl.handle.net/20.500.12123/29150048-96971879-1026https://doi.org/10.1016/j.scitotenv.2018.06.340Science of The Total Environment 645 : 34-43 (December 2018) In Progressreponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:22Zoai:localhost:20.500.12123/2915instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:23.068INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
title Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
spellingShingle Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
Demonte, Luisina Delma
Granjas Lecheras
Agua
Glifosato
Glufosinato
Experimentación
Dairy Farms
Water
Glyphosate
Glufosinate
Experimentation
Argentina
Tambos
title_short Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
title_full Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
title_fullStr Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
title_full_unstemmed Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
title_sort Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
dc.creator.none.fl_str_mv Demonte, Luisina Delma
Michlig, Nicolás
Gaggiotti, Monica Del Carmen
Adam, Claudia Guadalupe
Beldoménico, Horacio Ramón
Repetti, María Rosa
author Demonte, Luisina Delma
author_facet Demonte, Luisina Delma
Michlig, Nicolás
Gaggiotti, Monica Del Carmen
Adam, Claudia Guadalupe
Beldoménico, Horacio Ramón
Repetti, María Rosa
author_role author
author2 Michlig, Nicolás
Gaggiotti, Monica Del Carmen
Adam, Claudia Guadalupe
Beldoménico, Horacio Ramón
Repetti, María Rosa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Granjas Lecheras
Agua
Glifosato
Glufosinato
Experimentación
Dairy Farms
Water
Glyphosate
Glufosinate
Experimentation
Argentina
Tambos
topic Granjas Lecheras
Agua
Glifosato
Glufosinato
Experimentación
Dairy Farms
Water
Glyphosate
Glufosinate
Experimentation
Argentina
Tambos
dc.description.none.fl_txt_mv Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes.
EEA Rafaela
Fil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Adam, Claudia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. IQAL; Argentina
Fil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
description Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-30T14:16:12Z
2018-07-30T14:16:12Z
2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0048969718324161
http://hdl.handle.net/20.500.12123/2915
0048-9697
1879-1026
https://doi.org/10.1016/j.scitotenv.2018.06.340
url https://www.sciencedirect.com/science/article/pii/S0048969718324161
http://hdl.handle.net/20.500.12123/2915
https://doi.org/10.1016/j.scitotenv.2018.06.340
identifier_str_mv 0048-9697
1879-1026
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Science of The Total Environment 645 : 34-43 (December 2018) In Progress
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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