Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
- Autores
- Demonte, Luisina Delma; Michlig, Nicolás; Gaggiotti, Monica Del Carmen; Adam, Claudia Guadalupe; Beldoménico, Horacio Ramón; Repetti, María Rosa
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at
EEA Rafaela
Fil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Adam, Claudia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. IQAL; Argentina
Fil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina - Fuente
- Science of The Total Environment 645 : 34-43 (December 2018) In Progress
- Materia
-
Granjas Lecheras
Agua
Glifosato
Glufosinato
Experimentación
Dairy Farms
Water
Glyphosate
Glufosinate
Experimentation
Argentina
Tambos - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2915
Ver los metadatos del registro completo
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Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS methodDemonte, Luisina DelmaMichlig, NicolásGaggiotti, Monica Del CarmenAdam, Claudia GuadalupeBeldoménico, Horacio RamónRepetti, María RosaGranjas LecherasAguaGlifosatoGlufosinatoExperimentaciónDairy FarmsWaterGlyphosateGlufosinateExperimentationArgentinaTambosArgentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes.EEA RafaelaFil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); ArgentinaFil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); ArgentinaFil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Adam, Claudia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. IQAL; ArgentinaFil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina2018-07-30T14:16:12Z2018-07-30T14:16:12Z2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0048969718324161http://hdl.handle.net/20.500.12123/29150048-96971879-1026https://doi.org/10.1016/j.scitotenv.2018.06.340Science of The Total Environment 645 : 34-43 (December 2018) In Progressreponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:22Zoai:localhost:20.500.12123/2915instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:23.291INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
title |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
spellingShingle |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method Demonte, Luisina Delma Granjas Lecheras Agua Glifosato Glufosinato Experimentación Dairy Farms Water Glyphosate Glufosinate Experimentation Argentina Tambos |
title_short |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
title_full |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
title_fullStr |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
title_full_unstemmed |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
title_sort |
Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
dc.creator.none.fl_str_mv |
Demonte, Luisina Delma Michlig, Nicolás Gaggiotti, Monica Del Carmen Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Repetti, María Rosa |
author |
Demonte, Luisina Delma |
author_facet |
Demonte, Luisina Delma Michlig, Nicolás Gaggiotti, Monica Del Carmen Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Repetti, María Rosa |
author_role |
author |
author2 |
Michlig, Nicolás Gaggiotti, Monica Del Carmen Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Repetti, María Rosa |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Granjas Lecheras Agua Glifosato Glufosinato Experimentación Dairy Farms Water Glyphosate Glufosinate Experimentation Argentina Tambos |
topic |
Granjas Lecheras Agua Glifosato Glufosinato Experimentación Dairy Farms Water Glyphosate Glufosinate Experimentation Argentina Tambos |
dc.description.none.fl_txt_mv |
Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes. EEA Rafaela Fil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Adam, Claudia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. IQAL; Argentina Fil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina |
description |
Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07-30T14:16:12Z 2018-07-30T14:16:12Z 2018-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S0048969718324161 http://hdl.handle.net/20.500.12123/2915 0048-9697 1879-1026 https://doi.org/10.1016/j.scitotenv.2018.06.340 |
url |
https://www.sciencedirect.com/science/article/pii/S0048969718324161 http://hdl.handle.net/20.500.12123/2915 https://doi.org/10.1016/j.scitotenv.2018.06.340 |
identifier_str_mv |
0048-9697 1879-1026 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Science of The Total Environment 645 : 34-43 (December 2018) In Progress reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.623145 |