Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia
- Autores
- Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Galindo Sotomonte, Luisa Fernanda; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalfa rhizobia. Genomic analysis showed that most of the gene losses in inoculants are due to large genomic deletions rather than to small deletions or point mutations, a fact consistent with recurrent DNA double-strand breaks (DSBs) at numerous locations throughout the microbial genome. Genetic analysis showed that the loss of the NO-detoxifying enzyme HmpA in inoculants results in growth inhibition and high DSB levels under nitrosative stress, and large genomic deletions in planta but not in the soil. Therefore, besides its known function in the effective establishment of the symbiosis, HmpA can play a critical role in the preservation of the genomic integrity of alfalfa rhizobia under host-derived nitrosative stress.
Instituto de Biotecnología
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Galindo Sotomonte, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: McCormick, Wayne. Ottawa Research and Development Centre; Canadá
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Microbial Ecology 83 (2) : 501-505 (Febrero 2022)
- Materia
-
DNA Damage
Domestication
Nitric Oxide
Daño al ADN
Domesticación
Óxido Nítrico
Medicago sativa
Rhizobiaceae
Evolutionary Mechanism
Lucerne
Mecanismo Evolutivo
Alfalfa - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/11573
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Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa RhizobiaFrare, Romina AlejandraPascuan, Cecilia GabrielaGalindo Sotomonte, Luisa FernandaMcCormick, WayneSoto, Gabriela CynthiaAyub, Nicolás DanielDNA DamageDomesticationNitric OxideDaño al ADNDomesticaciónÓxido NítricoMedicago sativaRhizobiaceaeEvolutionary MechanismLucerneMecanismo EvolutivoAlfalfaWe have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalfa rhizobia. Genomic analysis showed that most of the gene losses in inoculants are due to large genomic deletions rather than to small deletions or point mutations, a fact consistent with recurrent DNA double-strand breaks (DSBs) at numerous locations throughout the microbial genome. Genetic analysis showed that the loss of the NO-detoxifying enzyme HmpA in inoculants results in growth inhibition and high DSB levels under nitrosative stress, and large genomic deletions in planta but not in the soil. Therefore, besides its known function in the effective establishment of the symbiosis, HmpA can play a critical role in the preservation of the genomic integrity of alfalfa rhizobia under host-derived nitrosative stress.Instituto de BiotecnologíaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Galindo Sotomonte, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: McCormick, Wayne. Ottawa Research and Development Centre; CanadáFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2022-04-06T15:32:27Z2022-04-06T15:32:27Z2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11573https://link.springer.com/article/10.1007/s00248-021-01761-41432-184Xhttps://doi.org/10.1007/s00248-021-01761-4Microbial Ecology 83 (2) : 501-505 (Febrero 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:31Zoai:localhost:20.500.12123/11573instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:31.683INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
title |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
spellingShingle |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia Frare, Romina Alejandra DNA Damage Domestication Nitric Oxide Daño al ADN Domesticación Óxido Nítrico Medicago sativa Rhizobiaceae Evolutionary Mechanism Lucerne Mecanismo Evolutivo Alfalfa |
title_short |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
title_full |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
title_fullStr |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
title_full_unstemmed |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
title_sort |
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
dc.creator.none.fl_str_mv |
Frare, Romina Alejandra Pascuan, Cecilia Gabriela Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author |
Frare, Romina Alejandra |
author_facet |
Frare, Romina Alejandra Pascuan, Cecilia Gabriela Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author_role |
author |
author2 |
Pascuan, Cecilia Gabriela Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
DNA Damage Domestication Nitric Oxide Daño al ADN Domesticación Óxido Nítrico Medicago sativa Rhizobiaceae Evolutionary Mechanism Lucerne Mecanismo Evolutivo Alfalfa |
topic |
DNA Damage Domestication Nitric Oxide Daño al ADN Domesticación Óxido Nítrico Medicago sativa Rhizobiaceae Evolutionary Mechanism Lucerne Mecanismo Evolutivo Alfalfa |
dc.description.none.fl_txt_mv |
We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalfa rhizobia. Genomic analysis showed that most of the gene losses in inoculants are due to large genomic deletions rather than to small deletions or point mutations, a fact consistent with recurrent DNA double-strand breaks (DSBs) at numerous locations throughout the microbial genome. Genetic analysis showed that the loss of the NO-detoxifying enzyme HmpA in inoculants results in growth inhibition and high DSB levels under nitrosative stress, and large genomic deletions in planta but not in the soil. Therefore, besides its known function in the effective establishment of the symbiosis, HmpA can play a critical role in the preservation of the genomic integrity of alfalfa rhizobia under host-derived nitrosative stress. Instituto de Biotecnología Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: McCormick, Wayne. Ottawa Research and Development Centre; Canadá Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalfa rhizobia. Genomic analysis showed that most of the gene losses in inoculants are due to large genomic deletions rather than to small deletions or point mutations, a fact consistent with recurrent DNA double-strand breaks (DSBs) at numerous locations throughout the microbial genome. Genetic analysis showed that the loss of the NO-detoxifying enzyme HmpA in inoculants results in growth inhibition and high DSB levels under nitrosative stress, and large genomic deletions in planta but not in the soil. Therefore, besides its known function in the effective establishment of the symbiosis, HmpA can play a critical role in the preservation of the genomic integrity of alfalfa rhizobia under host-derived nitrosative stress. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-06T15:32:27Z 2022-04-06T15:32:27Z 2022-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/11573 https://link.springer.com/article/10.1007/s00248-021-01761-4 1432-184X https://doi.org/10.1007/s00248-021-01761-4 |
url |
http://hdl.handle.net/20.500.12123/11573 https://link.springer.com/article/10.1007/s00248-021-01761-4 https://doi.org/10.1007/s00248-021-01761-4 |
identifier_str_mv |
1432-184X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Microbial Ecology 83 (2) : 501-505 (Febrero 2022) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.559606 |