Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa

Autores
Chudzinski Tavassi, Ana M.; Bermejo, Emilse; Rosenstein, Ruth Estela; Faria, Fernanda; Keller Sarmiento, María Inés; Alberto, Fabiana; Sapaio, Misako U.; Lazzari, María Ángela
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hementerin (HT) is an 80 kDa fibrino(geno)lytic metalloprotease, purified from saliva of the leech Haementeria depressa. In the present report, the effect of HT on several functional parameters of human platelets was assessed. HT inhibited platelet aggregation and ATP release induced by different agonists such as ADP, adrenaline, collagen, thrombin, and arachidonic acid. HT did neither modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV) nor intraplatelet fibrinogen levels, whereas it markedly decreased CD62P and CD63 levels after the stimulation with thrombin. HT significantly increased thrombin-induced platelet Ca2+ intracellular levels, cGMP content and nitric oxide synthase (NOS) activity. The effect of HT on platelet aggregation was reversed by two NOS inhibitors, Nω-Nitro-L-arginine methyl ester and 2 NG-Nitro-L-arginine. In summary, these results indicate that HT is an effective inhibitor of human platelet aggregation, presumably through activation of the platelet's nitridergic pathway.
Fil: Chudzinski Tavassi, Ana M.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Bermejo, Emilse. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Rosenstein, Ruth Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Faria, Fernanda. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Keller Sarmiento, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Alberto, Fabiana. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Sapaio, Misako U.. Universidade Federal de São Paulo; Brasil
Fil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Materia
Coagulation
Fibrinogenolysis
Nitric Oxide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66749
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66749
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressaChudzinski Tavassi, Ana M.Bermejo, EmilseRosenstein, Ruth EstelaFaria, FernandaKeller Sarmiento, María InésAlberto, FabianaSapaio, Misako U.Lazzari, María ÁngelaCoagulationFibrinogenolysisNitric Oxidehttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Hementerin (HT) is an 80 kDa fibrino(geno)lytic metalloprotease, purified from saliva of the leech Haementeria depressa. In the present report, the effect of HT on several functional parameters of human platelets was assessed. HT inhibited platelet aggregation and ATP release induced by different agonists such as ADP, adrenaline, collagen, thrombin, and arachidonic acid. HT did neither modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV) nor intraplatelet fibrinogen levels, whereas it markedly decreased CD62P and CD63 levels after the stimulation with thrombin. HT significantly increased thrombin-induced platelet Ca2+ intracellular levels, cGMP content and nitric oxide synthase (NOS) activity. The effect of HT on platelet aggregation was reversed by two NOS inhibitors, Nω-Nitro-L-arginine methyl ester and 2 NG-Nitro-L-arginine. In summary, these results indicate that HT is an effective inhibitor of human platelet aggregation, presumably through activation of the platelet's nitridergic pathway.Fil: Chudzinski Tavassi, Ana M.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Bermejo, Emilse. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Rosenstein, Ruth Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Faria, Fernanda. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Keller Sarmiento, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Alberto, Fabiana. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Sapaio, Misako U.. Universidade Federal de São Paulo; BrasilFil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaDe Gruyter2003-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66749Chudzinski Tavassi, Ana M.; Bermejo, Emilse; Rosenstein, Ruth Estela; Faria, Fernanda; Keller Sarmiento, María Inés; et al.; Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa; De Gruyter; Biological Chemistry; 384; 9; 9-2003; 1333-13391431-6730CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1515/BC.2003.150info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/bchm.2003.384.issue-9/bc.2003.150/bc.2003.150.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:28Zoai:ri.conicet.gov.ar:11336/66749instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:28.85CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
title Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
spellingShingle Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
Chudzinski Tavassi, Ana M.
Coagulation
Fibrinogenolysis
Nitric Oxide
title_short Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
title_full Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
title_fullStr Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
title_full_unstemmed Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
title_sort Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa
dc.creator.none.fl_str_mv Chudzinski Tavassi, Ana M.
Bermejo, Emilse
Rosenstein, Ruth Estela
Faria, Fernanda
Keller Sarmiento, María Inés
Alberto, Fabiana
Sapaio, Misako U.
Lazzari, María Ángela
author Chudzinski Tavassi, Ana M.
author_facet Chudzinski Tavassi, Ana M.
Bermejo, Emilse
Rosenstein, Ruth Estela
Faria, Fernanda
Keller Sarmiento, María Inés
Alberto, Fabiana
Sapaio, Misako U.
Lazzari, María Ángela
author_role author
author2 Bermejo, Emilse
Rosenstein, Ruth Estela
Faria, Fernanda
Keller Sarmiento, María Inés
Alberto, Fabiana
Sapaio, Misako U.
Lazzari, María Ángela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Coagulation
Fibrinogenolysis
Nitric Oxide
topic Coagulation
Fibrinogenolysis
Nitric Oxide
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Hementerin (HT) is an 80 kDa fibrino(geno)lytic metalloprotease, purified from saliva of the leech Haementeria depressa. In the present report, the effect of HT on several functional parameters of human platelets was assessed. HT inhibited platelet aggregation and ATP release induced by different agonists such as ADP, adrenaline, collagen, thrombin, and arachidonic acid. HT did neither modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV) nor intraplatelet fibrinogen levels, whereas it markedly decreased CD62P and CD63 levels after the stimulation with thrombin. HT significantly increased thrombin-induced platelet Ca2+ intracellular levels, cGMP content and nitric oxide synthase (NOS) activity. The effect of HT on platelet aggregation was reversed by two NOS inhibitors, Nω-Nitro-L-arginine methyl ester and 2 NG-Nitro-L-arginine. In summary, these results indicate that HT is an effective inhibitor of human platelet aggregation, presumably through activation of the platelet's nitridergic pathway.
Fil: Chudzinski Tavassi, Ana M.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Bermejo, Emilse. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Rosenstein, Ruth Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Faria, Fernanda. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Keller Sarmiento, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Alberto, Fabiana. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Sapaio, Misako U.. Universidade Federal de São Paulo; Brasil
Fil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
description Hementerin (HT) is an 80 kDa fibrino(geno)lytic metalloprotease, purified from saliva of the leech Haementeria depressa. In the present report, the effect of HT on several functional parameters of human platelets was assessed. HT inhibited platelet aggregation and ATP release induced by different agonists such as ADP, adrenaline, collagen, thrombin, and arachidonic acid. HT did neither modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV) nor intraplatelet fibrinogen levels, whereas it markedly decreased CD62P and CD63 levels after the stimulation with thrombin. HT significantly increased thrombin-induced platelet Ca2+ intracellular levels, cGMP content and nitric oxide synthase (NOS) activity. The effect of HT on platelet aggregation was reversed by two NOS inhibitors, Nω-Nitro-L-arginine methyl ester and 2 NG-Nitro-L-arginine. In summary, these results indicate that HT is an effective inhibitor of human platelet aggregation, presumably through activation of the platelet's nitridergic pathway.
publishDate 2003
dc.date.none.fl_str_mv 2003-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66749
Chudzinski Tavassi, Ana M.; Bermejo, Emilse; Rosenstein, Ruth Estela; Faria, Fernanda; Keller Sarmiento, María Inés; et al.; Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa; De Gruyter; Biological Chemistry; 384; 9; 9-2003; 1333-1339
1431-6730
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66749
identifier_str_mv Chudzinski Tavassi, Ana M.; Bermejo, Emilse; Rosenstein, Ruth Estela; Faria, Fernanda; Keller Sarmiento, María Inés; et al.; Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa; De Gruyter; Biological Chemistry; 384; 9; 9-2003; 1333-1339
1431-6730
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1515/BC.2003.150
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/bchm.2003.384.issue-9/bc.2003.150/bc.2003.150.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv De Gruyter
publisher.none.fl_str_mv De Gruyter
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614052011048960
score 13.070432

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