Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-inj...

Autores: Monti, Laura Lucia; Bustamante, Claudia Anabel; Budde, Claudio Olaf; Gabilondo, Julieta; Müller, Gabriela Leticia; Lara, Maria Valeria; Drincovich, María Fabiana
Año de publicación: 2019
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Fleshy fruit are susceptible to develop chilling injury (CI) disorders when stored at low temperatures for extended periods during the postharvest. In peach (Prunus persica L. Batsch) fruit, the woolly texture, expressed as a lack of juiciness, is the most important CI symptom. In the present work, we set up to study the metabolomic and proteomic differences of Spring Lady peach fruit, which, despite had been subjected to the same postharvest treatment (24 d at 0 °C followed by 5 d at 20 °C), display a contrasting woolliness phenotype. A drastic decrease in the content of sugars and sugar alcohols (sucrose, fructose, glucose, ribose, xylose, galactose, sorbitol and raffinose), as well as in all the amino acids detected (Ala, Asn, Gly, Glu, Ile, Ser, Thr, and Val), was found in woolly (WF) when compared to juicy fruit (JF). The quantitative proteomic analysis of JF and WF identified 227 differentially expressed proteins (DEP), from which 165 were increased and 62 decreased in WF with respect to JF. The identification of clusters of functionally associated proteins among the DEP revealed biochemical and physiological processes linked to the development of the woolly phenotype; such as sugar catabolism, amino acid usage, and proteome reconfiguration. On the other hand, a decrease of proteins involved in reactive oxygen species (ROS) detoxification, lower ascorbate peroxidase activity, and higher ROS levels, were found in the mesocarp of WF when compared to JF. Overall, this work identifies the induction of carbon oxidation processes, proteome reconfiguration, and increased ROS generation, as relevant metabolic signatures of woolliness development in peach fruit. Besides, the experimental approach used here clearly shows that the woolly phenotype can be triggered in similar biochemical contexts. The relevance of oxidative stress, probably as a consequence of an imbalance between ROS production and elimination, is highlighted as a causal mechanism of woolliness development in peach fruit.
EEA San Pedro
Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fuente: Postharvest Biology and Technology 151 : 142-151. (May 2019)
Materia: Durazno
Almacenamiento en Frío
Deterioro
Fisiología Postcosecha
Peaches
Cold Storage
Deterioration
Postharvest Physiology
Metaboloma
Proteoma
Woolliness
Daño por Frío
Metabolome
Proteome
Lanosidad
Chilling Injury
Nivel de accesibilidad: acceso restringido
Condiciones de uso
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/5211

Acceder

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spelling	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruitMonti, Laura LuciaBustamante, Claudia AnabelBudde, Claudio OlafGabilondo, JulietaMüller, Gabriela LeticiaLara, Maria ValeriaDrincovich, María FabianaDuraznoAlmacenamiento en FríoDeterioroFisiología PostcosechaPeachesCold StorageDeteriorationPostharvest PhysiologyMetabolomaProteomaWoollinessDaño por FríoMetabolomeProteomeLanosidadChilling InjuryFleshy fruit are susceptible to develop chilling injury (CI) disorders when stored at low temperatures for extended periods during the postharvest. In peach (Prunus persica L. Batsch) fruit, the woolly texture, expressed as a lack of juiciness, is the most important CI symptom. In the present work, we set up to study the metabolomic and proteomic differences of Spring Lady peach fruit, which, despite had been subjected to the same postharvest treatment (24 d at 0 °C followed by 5 d at 20 °C), display a contrasting woolliness phenotype. A drastic decrease in the content of sugars and sugar alcohols (sucrose, fructose, glucose, ribose, xylose, galactose, sorbitol and raffinose), as well as in all the amino acids detected (Ala, Asn, Gly, Glu, Ile, Ser, Thr, and Val), was found in woolly (WF) when compared to juicy fruit (JF). The quantitative proteomic analysis of JF and WF identified 227 differentially expressed proteins (DEP), from which 165 were increased and 62 decreased in WF with respect to JF. The identification of clusters of functionally associated proteins among the DEP revealed biochemical and physiological processes linked to the development of the woolly phenotype; such as sugar catabolism, amino acid usage, and proteome reconfiguration. On the other hand, a decrease of proteins involved in reactive oxygen species (ROS) detoxification, lower ascorbate peroxidase activity, and higher ROS levels, were found in the mesocarp of WF when compared to JF. Overall, this work identifies the induction of carbon oxidation processes, proteome reconfiguration, and increased ROS generation, as relevant metabolic signatures of woolliness development in peach fruit. Besides, the experimental approach used here clearly shows that the woolly phenotype can be triggered in similar biochemical contexts. The relevance of oxidative stress, probably as a consequence of an imbalance between ROS production and elimination, is highlighted as a causal mechanism of woolliness development in peach fruit.EEA San PedroFil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaElsevier2019-05-28T12:17:01Z2019-05-28T12:17:01Z2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0925521418310895http://hdl.handle.net/20.500.12123/52110925-5214https://doi.org/10.1016/j.postharvbio.2019.02.007Postharvest Biology and Technology 151 : 142-151. (May 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:48Zoai:localhost:20.500.12123/5211instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:50.494INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
title	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
spellingShingle	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit Monti, Laura Lucia Durazno Almacenamiento en Frío Deterioro Fisiología Postcosecha Peaches Cold Storage Deterioration Postharvest Physiology Metaboloma Proteoma Woolliness Daño por Frío Metabolome Proteome Lanosidad Chilling Injury
title_short	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
title_full	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
title_fullStr	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
title_full_unstemmed	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
title_sort	Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
dc.creator.none.fl_str_mv	Monti, Laura Lucia Bustamante, Claudia Anabel Budde, Claudio Olaf Gabilondo, Julieta Müller, Gabriela Leticia Lara, Maria Valeria Drincovich, María Fabiana
author	Monti, Laura Lucia
author_facet	Monti, Laura Lucia Bustamante, Claudia Anabel Budde, Claudio Olaf Gabilondo, Julieta Müller, Gabriela Leticia Lara, Maria Valeria Drincovich, María Fabiana
author_role	author
author2	Bustamante, Claudia Anabel Budde, Claudio Olaf Gabilondo, Julieta Müller, Gabriela Leticia Lara, Maria Valeria Drincovich, María Fabiana
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Durazno Almacenamiento en Frío Deterioro Fisiología Postcosecha Peaches Cold Storage Deterioration Postharvest Physiology Metaboloma Proteoma Woolliness Daño por Frío Metabolome Proteome Lanosidad Chilling Injury
topic	Durazno Almacenamiento en Frío Deterioro Fisiología Postcosecha Peaches Cold Storage Deterioration Postharvest Physiology Metaboloma Proteoma Woolliness Daño por Frío Metabolome Proteome Lanosidad Chilling Injury
dc.description.none.fl_txt_mv	Fleshy fruit are susceptible to develop chilling injury (CI) disorders when stored at low temperatures for extended periods during the postharvest. In peach (Prunus persica L. Batsch) fruit, the woolly texture, expressed as a lack of juiciness, is the most important CI symptom. In the present work, we set up to study the metabolomic and proteomic differences of Spring Lady peach fruit, which, despite had been subjected to the same postharvest treatment (24 d at 0 °C followed by 5 d at 20 °C), display a contrasting woolliness phenotype. A drastic decrease in the content of sugars and sugar alcohols (sucrose, fructose, glucose, ribose, xylose, galactose, sorbitol and raffinose), as well as in all the amino acids detected (Ala, Asn, Gly, Glu, Ile, Ser, Thr, and Val), was found in woolly (WF) when compared to juicy fruit (JF). The quantitative proteomic analysis of JF and WF identified 227 differentially expressed proteins (DEP), from which 165 were increased and 62 decreased in WF with respect to JF. The identification of clusters of functionally associated proteins among the DEP revealed biochemical and physiological processes linked to the development of the woolly phenotype; such as sugar catabolism, amino acid usage, and proteome reconfiguration. On the other hand, a decrease of proteins involved in reactive oxygen species (ROS) detoxification, lower ascorbate peroxidase activity, and higher ROS levels, were found in the mesocarp of WF when compared to JF. Overall, this work identifies the induction of carbon oxidation processes, proteome reconfiguration, and increased ROS generation, as relevant metabolic signatures of woolliness development in peach fruit. Besides, the experimental approach used here clearly shows that the woolly phenotype can be triggered in similar biochemical contexts. The relevance of oxidative stress, probably as a consequence of an imbalance between ROS production and elimination, is highlighted as a causal mechanism of woolliness development in peach fruit. EEA San Pedro Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
description	Fleshy fruit are susceptible to develop chilling injury (CI) disorders when stored at low temperatures for extended periods during the postharvest. In peach (Prunus persica L. Batsch) fruit, the woolly texture, expressed as a lack of juiciness, is the most important CI symptom. In the present work, we set up to study the metabolomic and proteomic differences of Spring Lady peach fruit, which, despite had been subjected to the same postharvest treatment (24 d at 0 °C followed by 5 d at 20 °C), display a contrasting woolliness phenotype. A drastic decrease in the content of sugars and sugar alcohols (sucrose, fructose, glucose, ribose, xylose, galactose, sorbitol and raffinose), as well as in all the amino acids detected (Ala, Asn, Gly, Glu, Ile, Ser, Thr, and Val), was found in woolly (WF) when compared to juicy fruit (JF). The quantitative proteomic analysis of JF and WF identified 227 differentially expressed proteins (DEP), from which 165 were increased and 62 decreased in WF with respect to JF. The identification of clusters of functionally associated proteins among the DEP revealed biochemical and physiological processes linked to the development of the woolly phenotype; such as sugar catabolism, amino acid usage, and proteome reconfiguration. On the other hand, a decrease of proteins involved in reactive oxygen species (ROS) detoxification, lower ascorbate peroxidase activity, and higher ROS levels, were found in the mesocarp of WF when compared to JF. Overall, this work identifies the induction of carbon oxidation processes, proteome reconfiguration, and increased ROS generation, as relevant metabolic signatures of woolliness development in peach fruit. Besides, the experimental approach used here clearly shows that the woolly phenotype can be triggered in similar biochemical contexts. The relevance of oxidative stress, probably as a consequence of an imbalance between ROS production and elimination, is highlighted as a causal mechanism of woolliness development in peach fruit.
publishDate	2019
dc.date.none.fl_str_mv	2019-05-28T12:17:01Z 2019-05-28T12:17:01Z 2019
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://www.sciencedirect.com/science/article/pii/S0925521418310895 http://hdl.handle.net/20.500.12123/5211 0925-5214 https://doi.org/10.1016/j.postharvbio.2019.02.007
url	https://www.sciencedirect.com/science/article/pii/S0925521418310895 http://hdl.handle.net/20.500.12123/5211 https://doi.org/10.1016/j.postharvbio.2019.02.007
identifier_str_mv	0925-5214
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv	restrictedAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	Postharvest Biology and Technology 151 : 142-151. (May 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
collection	INTA Digital (INTA)
instname_str	Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-inj...

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